Dynamic reorganization of Eg5 in the mammalian spindle throughout mitosis requires dynein and TPX2

The kinesin Eg5 moves toward minus ends of astral microtubules in early mitosis, switching to plus-end motion in anaphase. Dynein is required for minus-end motion; depletion of TPX2 results in a switch to plus-end motion. On midzone microtubules, Eg5 moves in both directions. Our results explain the redistribution of Eg5 throughout mitosis

Poleward Transport of TPX2 in the Mammalian Mitotic Spindle Requires Dynein, Eg5, and Microtubule Flux

TPX2 is a spindle assembly factor that is required for MT assembly near chromosomes. Using photoactivation of fluorescence, we report that TPX2 is transported poleward in the half-spindle. Poleward transport of TPX2 is sensitive to inhibition of dynein or Eg5, and to suppression of MT flux