Photographing the Earth G324: The Can Do GeoCam payload

The flight of the Charleston County School District Can Do Project GeoCam payload on STS-57 was the climax of a decade long endeavor to bring the promise and excitement of the space program directly into the classroom. The payload carried four cameras designed to take high resolution photographs of the Earth under the direction of children operating the first ever student control room. During the course of the flight, the students followed the Shuttle's orbital tract, satellite weather images and selected a target list that was sent up to the crew each night as part of the execute package. Targets from this list, as well as ones chosen by the crew visually, resulted in the successful collection of photographic runs at many interesting sites on three on three continents

Testing for Features in the Primordial Power Spectrum

Well-known causality arguments show that events occurring during or at the end of inflation, associated with reheating or preheating, could contribute a blue component to the spectrum of primordial curvature perturbations, with the dependence k^3. We explore the possibility that they could be observably large in CMB, LSS, and Lyman-alpha data. We find that a k^3 component with a cutoff at some maximum k can modestly improve the fits (Delta chi^2=2.0, 5.4) of the low multipoles (l ~ 10 - 50) or the second peak (l ~ 540) of the CMB angular spectrum when the three-year WMAP data are used. Moreover, the results from WMAP are consistent with the CBI, ACBAR, 2dFGRS, and SDSS data when they are included in the analysis. Including the SDSS galaxy clustering power spectrum, we find weak positive evidence for the k^3 component at the level of Delta chi' = 2.4, with the caveat that the nonlinear evolution of the power spectrum may not be properly treated in the presence of the k^3 distortion. To investigate the high-k regime, we use the Lyman-alpha forest data (LUQAS, Croft et al., and SDSS Lyman-alpha); here we find evidence at the level Delta chi^2' = 3.8. Considering that there are two additional free parameters in the model, the above results do not give a strong evidence for features; however, they show that surprisingly large bumps are not ruled out. We give constraints on the ratio between the k^3 component and the nearly scale-invariant component, r_3 < 1.5, over the range of wave numbers 0.0023/Mpc < k < 8.2/Mpc. We also discuss theoretical models which could lead to the k^3 effect, including ordinary hybrid inflation and double D-term inflation models. We show that the well-motivated k^3 component is also a good representative of the generic spikelike feature in the primordial perturbation power spectrum.Comment: 23 pages, 6 figures; added new section on theoretical motivation for k^3 term, and discussion of double D-term hybrid inflation models; title changed, added a new section discussing the generic spikelike features, published in IJMP

Small passive student experiments on G324 261 individual quests for student knowledge

The Charleston County School District CAN DO Project payload on STS-57 had a primary goal of photographing the Earth with the GeoCam camera system. In addition, the payload carried 261 passive student experiments representing the efforts of several thousand students throughout the district and in four other states. These experiments represented the individual concepts of teams ranging in age from pre-school to high school. Consequently, a tremendous variety of samples from collard green seeds to microscopic 'water bears' were flown. Each prospective team was provided a simple kit equipped with five vials. Each student team submitted five coded samples, one for space flight and four control samples. The control samples were exposed to radiation, cold and centrifugation respectively while one negative control sample was passively stored. The students received the samples back still coded so that they were unaware of which samples were flown. They then investigated their samples according to their individual research protocols. The results were presented in poster and platform form at a student research symposium. Space Trees grown from tree seeds flown in the payload have been planted at all district schools, and at many guest schools. These seeds represented another way in which to involve additional classes and students. Both the passive experiments and the space trees were housed in what otherwise would have been wasted space within the payload. They extended the GAS programs worthwhile ballast concept to another level. The opportunity to fly an experiment in space is too previous not to be extended to the greatest number of students possible

Orbit Determination and Navigation of the Solar Terrestrial Relations Observatory (STEREO)

This paper provides an overview of the required upgrades necessary for navigation of NASA's twin heliocentric science missions, Solar TErestrial RElations Observatory (STEREO) Ahead and Behind. The orbit determination of the STEREO spacecraft was provided by the NASA Goddard Space Flight Center's (GSFC) Flight Dynamics Facility (FDF) in support of the mission operations activities performed by the Johns Hopkins University Applied Physics Laboratory (APL). The changes to FDF's orbit determination software included modeling upgrades as well as modifications required to process the Deep Space Network X-band tracking data used for STEREO. Orbit results as well as comparisons to independently computed solutions are also included. The successful orbit determination support aided in maneuvering the STEREO spacecraft, launched on October 26, 2006 (00:52 Z), to target the lunar gravity assists required to place the spacecraft into their final heliocentric drift-away orbits where they are providing stereo imaging of the Sun

Characterising Inter-helical Interactions of G Protein-Coupled Receptors with the Fragment Molecular Orbital Method

G-protein coupled receptors (GPCRs) are the largest superfamily of membrane proteins, regulating almost every aspect of cellular activity and serving as key targets for drug discovery. We have identified an accurate and reliable computational method to characterise the strength and chemical nature of the inter-helical interactions between the residues of transmembrane (TM) domains during different receptor activation states, something that cannot be characterised solely by visual inspection of structural information. Using the fragment molecular orbital (FMO) quantum mechanics method to analyse 35 crystal structures representing different branches of the class A GPCR family, we have identified 69 topologically-equivalent TM residues that form a consensus network of 51 inter-TM interactions, providing novel results that are consistent with and help to rationalise experimental data. This discovery establishes a comprehensive picture of how defined molecular forces govern specific inter-helical interactions which, in turn, support the structural stability, ligand binding and activation of GPCRs

A comparison of collision cross section values obtained via travelling wave ion mobility-mass spectrometry and ultra high performance liquid chromatography-ion mobility-mass spectrometry : application to the characterisation of metabolites in rat urine

A comprehensive Collision Cross Section (CCS) library was obtained via Travelling Wave Ion Guide mobility measurements through direct infusion (DI). The library consists of CCS and Mass Spectral (MS) data in negative and positive ElectroSpray Ionisation (ESI) mode for 463 and 479 endogenous metabolites, respectively. For both ionisation modes combined, TWCCSN2 data were obtained for 542 non-redundant metabolites. These data were acquired on two different ion mobility enabled orthogonal acceleration QToF MS systems in two different laboratories, with the majority of the resulting TWCCSN2 values (from detected compounds) found to be within 1% of one another. Validation of these results against two independent, external TWCCSN2 data sources and predicted TWCCSN2 values indicated to be within 1-2% of these other values. The same metabolites were then analysed using a rapid reversed-phase ultra (high) performance liquid chromatographic (U(H)PLC) separation combined with IM and MS (IM-MS) thus providing retention time (tr), m/z and TWCCSN2 values (with the latter compared with the DI-IM-MS data). Analytes for which TWCCSN2 values were obtained by U(H)PLC-IM-MS showed good agreement with the results obtained from DI-IM-MS. The repeatability of the TWCCSN2 values obtained for these metabolites on the different ion mobility QToF systems, using either DI or LC, encouraged the further evaluation of the U(H)PLC-IM-MS approach via the analysis of samples of rat urine, from control and methotrexate-treated animals, in order to assess the potential of the approach for metabolite identification and profiling in metabolic phenotyping studies. Based on the database derived from the standards 63 metabolites were identified in rat urine, using positive ESI, based on the combination of tr, TWCCSN2 and MS data.</p

Paediatric extracranial germ-cell tumours.

Management of paediatric extracranial germ-cell tumours carries a unique set of challenges. Germ-cell tumours are a heterogeneous group of neoplasms that present across a wide age range and vary in site, histology, and clinical behaviour. Patients with germ-cell tumours are managed by a diverse array of specialists. Thus, staging, risk stratification, and treatment approaches for germ-cell tumours have evolved disparately along several trajectories. Paediatric germ-cell tumours differ from the adolescent and adult disease in many ways, leading to complexities in applying age-appropriate, evidence-based care. Suboptimal outcomes remain for several groups of patients, including adolescents, and patients with extragonadal tumours, high tumour markers at diagnosis, or platinum-resistant disease. Survivors have significant long-term toxicities. The challenge moving forward will be to translate new insights from molecular studies and collaborative clinical data into improved patient outcomes. Future trials will be characterised by improved risk-stratification systems, biomarkers for response and toxic effects, rational reduction of therapy for low-risk patients and novel approaches for poor-risk patients, and improved international collaboration across paediatric and adult cooperative research groups.This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/S1470-2045(15)00545-

Timing of deployment does not affect the biodiversity outcomes of ecological enhancement of coastal flood defences in northern Europe

Timing of installation is an important factor when planning the deployment of ecological enhancements to intertidal coastal and marine infrastructure. Such nature-based solutions (NbS) are increasingly used worldwide, so understanding whether the timing of deployment affects colonisation success is crucial to enhance their success and identify any ecological sensitivities that must be taken into consideration during construction. To date, none of the previous marine eco-engineering studies globally have looked specifically at timing. An unexpected COVID19 interruption in retrofitting Ecotiles designed to improve urban marine biodiversity provided a unique window of opportunity to address this research gap. We examined if time of deployment affects the early colonisation (within 18 months) success of eco-engineering enhancements. Thirty concrete tiles (Ecotiles) cast with a novel multi-scale, multi-species textured formliner were deployed on rock armour in three sites along the coast in Edinburgh, Scotland, at two different time periods (early March and late May 2020). After two settlement seasons, the colonisation success of 85% of the studied species did not vary between the times of deployment. Early colonisation success of intertidal species equalised within two settlement seasons of deployment, along with an overall increase in species richness. Crucially, these results also show that summer construction periods designed to reduce impacts on overwintering birds, do not adversely impact intertidal species during their peak (spring-summer) recruitment period in northern Europe. This novel result provides further support for widespread use of eco-engineering to enhance large coastal infrastructure projects and achieve ecological goals in northern Europe. More widely, this work contributes to the understanding of the impact of deployment timing on the success of similar NbS worldwide