Regulacija sile hidrauličke preše pomoću programa LabVIEW i uređaja NI-myRIO

U ovom završnom radu načinjen je eksperiment regulacije sile hidrauličke preše pomoću programa LabVIEW i uređaja NI myRIO. LabVIEW je grafički programski jezik namijenjen lakšem sakupljanju podataka iz ljudske okoline sa raznih instrumenata. Najčešće se koristi za prikupljanje, analizu i obradu podataka te kontrolu instrumenata i opreme. Programi rađeni pomoću LabVIEW alata nazivaju se Virtualni Instrumenti, skraćeno VI (engl. Virtual Instruments), jer njihov izgled i rad imitira stvarne instrumente. Svaki VI se sastoji od prednjeg panela i blok dijagrama. Prednji panel (engl. Front panel) namijenjen je za izradu grafičkog sučelja koji korisnik vidi kada je sustav pokrenut, a blok dijagram (engl. Block diagram) sadrži programske elemente preko kojih se odvija obrada podataka. S elektro-hidrauličkim servo sustavom povezan je uređaj NI myRIO na kojem se izvršava upravljački program za regulaciju sile hidrauličke preše primjenom servo ventila. NI myRIO je laboratorijski uređaj koji omogućuje izvršavanje algoritma u stvarnom realnom vremenu. Za vizualizaciju i nadzor rada elektro-hidrauličkog servo sustava koristi se prijenosno računalo na kojem se nalazi grafičko sučelje

Characterization of autophagosome initiation- (a) and elongation-related genes (b) in various tissues of male and female Red Jungle Fowl (<i>Gallus gallus</i>) by RT-qPCR as described in materials and methods.

<p>Signals were visualized by agarose gel electrophoresis.</p

Relative expression of autophagosome initiation-related genes in various tissues of R ans S male Japonica quail lines.

<p>Total RNA from each tissue was DNAse-treated, reverse transcribed, and subjected to real-time quantitative PCR. Sample were run in duplicate, and the average threshold cycle (Ct) values were determined for the target and houskeeping genes. Relative quantity of autophagy genes was determined by the 2^−ΔΔCt method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112449#pone.0112449-Davis1" target="_blank">[58]</a>. Data are presented as mean ± SEM (n = 6 for each line and each tissue). * Line-matched differences among tissues (*<i>P</i><0.05). Different letters indicate tissue-matched differences among Lines (a–c, difference between tissues within R line and α-ε indicate differences between tissues within S line).</p

Single animal breakdown situations (sabs) - application of a model

Department of Agriculture, Food and the MarineTeagascDeposited by bulk impor

Comparison of relative expression of autophagosome initiation-related genes in various tissues of male and female Red Jungle Fowl.

<p>Total RNA from each tissue was DNAse-treated, reverse transcribed, and subjected to real-time quantitative PCR as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112449#s2" target="_blank">material and methods</a>. Samples were run in duplicate, and the average threshold cycle (Ct) values were determined for the target and houskeeping genes. Relative quantity of autophagy genes was determined by the 2^−ΔΔCt method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112449#pone.0112449-Davis1" target="_blank">[58]</a>. Data are presented as mean ± SEM (n = 6 for each gender and each tissue). * Sex-matched differences among tissues (*<i>P</i><0.05 and **<i>P</i><0.01). Different letters indicate tissue-matched differences among gender (a–e, difference between tissues within female and α-δ indicate differences between male tissues).</p

Comparison of relative expression of autophagosome elongation-related genes in various tissues of R and S male Japonica quail lines.

<p>Total RNA from each tissue was DNAse-treated, reverse transcribed, and subjected to real-time quantitative PCR. Sample were run in duplicate, and the average threshold cycle (Ct) values were determined for the target and houskeeping genes. Relative quantity of autophagy genes was determined by the 2^−ΔΔCt method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112449#pone.0112449-Davis1" target="_blank">[58]</a>. Data are presented as mean ± SEM (n = 6 for each line and each tissue). * Genotype-matched differences among tissues (*<i>P</i><0.05 and ***<i>P</i><0.001). Different letters indicate tissue-matched differences among genotype (a, b, difference between tissues within R line and α-β indicate differences between tissues within S line). 28.</p

Phylogenetic relationships among chicken autophagy-related genes and their mammalian orthologs were inferred using the neighbor-joining method in MUSCLE alignment and MEGA6.

<p>Scale bar indicates the substitution rate per residue. Genbank accession numbers are included in the phenogram and in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112449#pone-0112449-t002" target="_blank">Table 2</a>.</p

Characterization of autophagosome initiation- (a) and elongation-related genes (b) in various tissues of stress-sensitive (S) and stress-resistant (R) male Japonica quail (<i>Coturnix coturnix Japonica</i>) using RT-qPCR.

<p>Signals were visualized by agarose gel electrophoresis.</p

Oligonucleotide PCR primers.

a<p>Accession number refer to Genbank (NCBI).</p><p>Oligonucleotide PCR primers.</p