Semen analysis of G olden R etriever healthy dogs and those affected by muscular dystrophy

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106077/1/and12079.pd

Evaluation of lasting effects of heat stress on sperm profile and oxidative status of ram semen and epididymal sperm

Higher temperatures lead to an increase of testicular metabolism that results in spermatic damage. Oxidative stress is the main factor responsible for testicular damage caused by heat stress. The aim of this study was to evaluate lasting effects of heat stress on ejaculated sperm and immediate or long-term effects of heat stress on epididymal sperm. We observed decrease in motility and mass motility of ejaculated sperm, as well as an increase in the percentages of sperm showing major and minor defects, damaged plasma and acrosome membranes, and a decrease in the percentage of sperm with high mitochondrial membrane potential in the treated group until one spermatic cycle. An increased enzymatic activity of glutathione peroxidase and an increase of stressed cells were observed in ejaculated sperm of the treated group. A decrease in the percentage of epididymal sperm with high mitochondrial membrane potential was observed in the treated group. However, when comparing immediate and long-term effects, we observed an increase in the percentage of sperm with low mitochondrial membrane potential. In conclusion, testicular heat stress induced oxidative stress that led to rescuable alterations after one spermatic cycle in ejaculated sperm and also after 30 days in epididymal sperm

Population synthesis of millisecond and submillisecond pulsars

Known millisecond pulsars have periods longer than 1.558 ms. Recycled in binary systems, neutron stars can attain very short spin periods. In this paper we investigate the expected properties of the millisecond pulsar distribution by simulating synthetic populations under different assumptions for the neutron star equation of state and decay of the magnetic field. We find evidence that a tail in the distribution of millisecond pulsars may exist at periods shorter than those observed.Comment: 16 pages, LaTex + 2 .eps figures To Appear in The Astrophysical Journal Letter

Sperm cryodamage occurs after rapid freezing phase: flow cytometry approach and antioxidant enzymes activity at different stages of cryopreservation

Abstract\ud \ud Background\ud In order to improve the efficiency of bovine sperm cryopreservation process, it is important to understand how spermatozoa respond to differences in temperature as well as the ability to recover its own metabolism. The combination between flow cytometry approach and antioxidant enzymes activity allows a more sensible evaluation of sperm cell during cryopreservation. The aim of this study was to evaluate sperm attributes and antioxidant enzymes activity during different stages of cryopreservation process. Semen samples from Holstein bulls (n = 4) were separated in 3 treatments: fresh (37 °C); cooled (5 °C); and thawed. Evaluation occurred at 0 h and 2 h after incubation. Membrane integrity, mitochondrial membrane potential (MMP) and DNA damages were evaluated by flow cytometry; activities of antioxidant enzymes such as catalase, superoxide dismutase and gluthatione peroxidase were measured by spectrofotometry.\ud \ud \ud Results\ud There was an increase in the percentage of sperm with DNA damage in the thawed group, compared to fresh and cooled, and for 2 hs of incubation when compared to 0 h. Considering MMP, there was an increase in the percentage of cells with medium potential in thawed group when compared to fresh and cooled groups. Opposingly, a decrease was observed in the thawed group considering high mitochondrial potential. Also in the thawed group, there was an increase on cells with damaged acrosome and membrane when compared to fresh and cooled groups. Significant correlations were found between antioxidant enzymes activity and membrane or mitochondrial parameters.\ud \ud \ud Conclusion\ud Based on our results, we conclude that cryopreservation affects cellular and DNA integrity and that the critical moment is when sperm cells are exposed to freezing temperature. Also, our study indicates that intracellular antioxidant machinery (SOD and GPX enzymes) is not enough to control cryodamage.This study was supported by São Paulo Research Foundation (FAPESP),\ud process number 2010/18978-9 and 2007/58487-1

Where May Ultra-Fast Rotating Neutron Stars Be Hidden?

The existence of ultra-fast rotating neutron stars (spin period P < 1 ms) is expected on the basis of current models for the secular evolution of interacting binaries, though they have not been detected yet. Their formation depends on the quantity of matter accreted by the neutron star which, in turn, is limited by the mechanism of mass ejection from the binary. An efficient mass ejection can avoid the formation of ultra-fast pulsars or their accretion induced collapse to a black hole. We propose that significant reductions of the mass-transfer rate may cause the switch-on of a radio pulsar phase, whose radiation pressure may be capable of ejecting out of the system most of the matter transferred by the companion. This can prevent, for long orbital periods and if a sufficiently fast spin has been reached, any further accretion, even if the original transfer rate is restored, thus limiting the minimum spin period attainable by the neutron star. We show that close systems (orbital periods P_orb \sim 1 hr are the only possible hosts for ultra-fast spinning neutron stars. This could explain why ultra-fast radio pulsars have not been detected so far, as the detection of pulsars with very short spin periods in close systems is hampered, in current radio surveys, by strong Doppler modulation and computational limitations.Comment: 6 pages, including 1 figure. To appear in ApJ

Radio Pulsars in the Magellanic Clouds

We report the results of a survey of the Small Magellanic Cloud (SMC) for radio pulsars conducted with the 20-cm multibeam receiver of the Parkes 64-meter telescope. This survey targeted a more complete region of the SMC than a previous pulsar search and had an improvement in sensitivity by a factor of about two for most pulsar periods. This survey is much more sensitive to fast young pulsars (with P < 100 ms) and is the first survey of the SMC with any sensitivity to millisecond pulsars. Two new pulsars were discovered in the survey, one of which is located within the SMC. The number of pulsars found in the survey is consistent with the expected number derived using several methods. We also report the serendipitous discovery of a new pulsar in the 30 Doradus region of the Large Magellanic Cloud (LMC). These discoveries bring the total number of rotation-powered pulsars currently known in the Magellanic Clouds to eight. We have also made refined timing measurements for the new discoveries as well as for three previously known LMC pulsars. The age distribution of luminous Magellanic Cloud pulsars supports the conjecture that pulsars younger than about 5 Myr are more luminous on average than older pulsars.Comment: 14 pages, including 6 figures and 3 tables. Accepted for publication in Ap

Deontological issues - possible misdiagnosis of cerebral metastases

Authors analyses a number of 4588 (52, 24% over 50 years old) patients operated for cerebral tumors in the Clinic Emergency Hospital “Bagdasar-Arseni” from Bucharest, between 2000-2010, with peculiar attention to the concordance between the preoperative and postoperative diagnosis, related to the actual policy to evaluate a neurosurgical patient before surgery. 903 cases were cerebral metastases and 69,5% aged over 50 years old. In 9,7% of cases we recorded a preoperative misdiagnosis of a metastasis due to few main reasons: unavailable information about a present primitive cancer, treacherous MRI image with a single confusing appearance of a cerebral lesion, age less than 50 years old, clinical presentation and biological evaluation inconsistent with malignancy. Authors point that these situations can have serious consequences related to professional competence, deterioration of the patient-doctor relationship, increasing costs for completion of diagnosis and treatment, and inadequate information about patient’s prognosis