Are the pentaquark sum rules reliable?

We rewiew and scrutinize the existing mass determinations of the pentaquarks from the exponential Laplace Sum Rules (LSR). We do not find any sum rule window for extracting optimal and reliable results from the LSR, due to the unusual slow convergence of the OPE and to the exceptional important role of the QCD continuum into the spectral function in this channel. Instead, we use in this channel,for the first time, Finite Energy Sum Rules (FESR), which exhibit a nice stability in the QCD continuum threshold t_c, at which one can extract, with a good accuracy, the mass of the lowest resonance. Including the D=7, 9 condensate contributions in the OPE, we obtain M_Theta=(1513+- 114) MeV, and the corresponding residue lambda_Theta^2= -(0.14-- 0.49)x 10^{-9} GeV^{12}, which favours the I=0, J=1/2, and negative parity S-wave interpretation of the Theta (1540). However, our analysis indicates a degeneracy between the unmixed I=0 and I=1 S-wave states. In the I=0, J=1/2, P-wave channel, we obtain, for the P-resonance, M_P = (1.99+- 0.19) GeV and lambda_P= -(0.7--7.1)x 10^{-9} GeV^{14}, which we expect to be discovered experimentally. Our results also suggest that some intuitive choices of the continuum threshold used in the LSR literature are inconsistent with the FESR results. Finally, a study of the Theta-K-N coupling using a vertex sum rule shows that, for the I=0, S-wave channel, the leading OPE contributions only start to order alpha_s^2 in the chiral limit m_s=0, indicating that the Theta is very narrow.Comment: Latex file 6 pages, 5 ps.figures. Contribution to the QCD 04 International Conference (Montpellier-5-9th July 2004) and to HEP-MAD 04 International Conference (Antananarivo 27th Sept-2nd Oct. 2004). To appear in Nucl. Phys. B (Proc. Suppl.) and in SLAC Econf. on-line Proceedings. Comments and References adde

FETR-ALS Study Protocol: A Randomized Clinical Trial of Fecal Microbiota Transplantation in Amyotrophic Lateral Sclerosis

Background and Rationale: Among the key players in the pathogenesis of Amyotrophic Lateral Sclerosis (ALS), microglia and T regulatory lymphocytes (Treg) are candidate cells for modifying the course of the disease. The gut microbiota (GM) acts by shaping immune tolerance and regulating the Treg number and suppressive function, besides circulating neuropeptides, and other immune cells that play in concert through the gut-brain axis. Previous mouse models have shown an altered enteric flora in early stage ALS, pointing to a possible GM role in ALS pathogenesis. Fecal Microbial Transplantation (FMT) is a well-known therapeutic intervention used to re-establish the proper microenvironment and to modulate enteric and systemic immunity. Methods: We are going to perform a multicenter randomized double-blind clinical trial employing FMT as a therapeutic intervention for ALS patients (NCT0376632). Forty-two ALS patients, at an early stage, will be enrolled with a 2:1 allocation ratio (28 FMT-treated patients vs. 14 controls). Study duration will be 12 months per patient. Three endoscopic procedures for intestinal biopsies in FMT and control groups are predicted at baseline, month 6 and month 12; at baseline and at month 6 fresh feces from healthy donors will be infused at patients in the intervention arm. The primary outcome is a significant change in Treg number between FMT-treated patients and control arm from baseline to month 6. Secondary outcomes include specific biological aims, involving in-depth analysis of immune cells and inflammatory status changes, central and peripheral biomarkers of ALS, besides comprehensive analysis of the gut, saliva and fecal microbiota. Other secondary aims include validated clinical outcomes of ALS (survival, forced vital capacity, and modifications in ALSFRS-R), besides safety and quality of life. Expected Results: We await FMT to increase Treg number and suppressive functionality, switching the immune system surrounding motorneurons to an anti-inflammatory, neuroprotective status. Extensive analysis on immune cell populations, cytokines levels, and microbiota (gut, fecal and saliva) will shed light on early processes possibly leading the degenerative ALS course. Conclusions: This is the first trial with FMT as a potential intervention to modify immunological response to ALS and disease progression at an early stage

Single and Double Spin Asymmetries for Deeply Virtual Compton Scattering Measured with CLAS and a Longitudinally Polarized Proton Target

Single-beam, single-target, and double spin asymmetries for hard exclusive electroproduction of a photon on the proton →e→p~ → e\u27p\u27γ are presented. The data were taken at Jefferson Lab using the CEBAF large acceptance spectrometer and a longitudinally polarized 14NH3 target. The three asymmetries were measured in 165 four-dimensional kinematic bins, covering the widest kinematic range ever explored simultaneously for beam and target-polarization observables in the valence quark region. The kinematic dependences of the obtained asymmetries are discussed and compared to the predictions of models of generalized parton distributions. The measurement of three DVCS spin observables at the same kinematic points allows a quasi-model-independent extraction of the imaginary parts of the H and ~H Compton form factors, which give insight into the electric and axial charge distributions of valence quarks in the proton

Potential Role of M. tuberculosis Specific IFN-γ and IL-2 ELISPOT Assays in Discriminating Children with Active or Latent Tuberculosis

BACKGROUND: Although currently available IGRA have been reported to be promising markers for TB infection, they cannot distinguish active tuberculosis (TB) from latent infection (LTBI). OBJECTIVE: Children with LTBI, active TB disease or uninfected were prospectively evaluated by an in-house ELISPOT assay in order to investigate possible immunological markers for a differential diagnosis between LTBI and active TB. METHODS: Children at risk for TB infection prospectively enrolled in our infectious disease unit were evaluated by in-house IFN-γ and IL-2 based ELISPOT assays using a panel of Mycobacterium tuberculosis antigens. RESULTS: Twenty-nine children were classified as uninfected, 21 as LTBI and 25 as active TB cases (including 5 definite and 20 probable cases). Significantly higher IFN-γ ELISPOT responses were observed in infected vs. uninfected children for ESAT-6 (p<0.0001), CFP-10 (p<0.0001), TB 10.3 (p = 0.003), and AlaDH (p = 0.001), while differences were not significant considering Ag85B (p = 0.063), PstS1 (p = 0.512), and HspX (16 kDa) (p = 0.139). IL-2 ELISPOT assay responses were different for ESAT-6 (p<0.0001), CFP-10 (p<0.0001), TB 10.3 (p<0.0001), HspX (16 kDa) (p<0.0001), PstS1 (p<0.0001) and AlaDH (p = 0.001); but not for Ag85B (p = 0.063). Comparing results between children with LTBI and those with TB disease differences were significant for IFN-γ ELISPOT only for AlaDH antigen (p = 0.021) and for IL-2 ELISPOT assay for AlaDH (p<0.0001) and TB 10.3 antigen (p = 0.043). ROC analyses demonstrated sensitivity of 100% and specificity of 81% of AlaDH-IL-2 ELISPOT assay in discriminating between latent and active TB using a cut off of 12.5 SCF per million PBMCs. CONCLUSION: Our data suggest that IL-2 based ELISPOT with AlaDH antigen may be of help in discriminating children with active from those with latent TB