Immunohistochemical Localization of Dendritic Cells in Human Dental Pulp

Svrha: Svrha istraživanja bila je metodom imunohistokemijskih bojenja locirati dendritičke stanice, te dio imunokompetentnog sustava dentalne pulpe u histološkim uzorcima normalne i ireverzibilno inflamirane ljudske zubne pulpe. Materijal i metode: Zbog posebnih svojstava vezivnog tkiva ljudske zubne pulpe, koristila su se sljedeća antitijela: vimentin V9, protein povezan s neurofi lamentima (NFAP), protein S 100 te aktin glatkih mišića (klon 1A4). Rezultati: Rezultati su pokazali da humana dentalna pulpa ima mnogobrojne stanice s razgranatim citoplazmatskim produžecima u području upalnoga infiltrata. Na temelju imunohistokemijskog bojenja smatramo da je riječ o dendritičkim stanicama. Zaključak: Dendritičke stanice u upaljenoj ljudskoj zubnoj pulpi nisu neočekivane s obzirom na veliku gustoću upalnog infiltrata i ulogu koju imaju u upalnom odgovoru. Podtip i značenje tih stanica treba još objasniti.Objectives: The aim of this study was to locate the dendritic cells, a part of the immunocompetent system of dental pulp, in histological samples of normal and irreversibly inflamed human dental pulp, using immunohistochemical staining methods. Material and Methods: In light of the particular character of the connective tissue of human dental pulp, the following antibodies were used: vimentin clone V9, a protein associated with neurofilaments (NFAP), the S 100 protein, and smooth muscle type actin (clone 1A4). Results: The results indicated that human dental pulp has numerous cells with branching cytoplasmic extensions, in areas of inflammatory infiltrate. Based on immunohistochemical staining we consider these to be dendritic cells. Conclusions: The presence of dendritic cells in inflamed human dental pulp is not unexpected, considering the great density of the inflammatory infiltrate, and the role they play in the inflammatory response. The subtype and significance of these cells need to be clarified

Immunohistochemical Localization of Dendritic Cells in Human Dental Pulp

Svrha: Svrha istraživanja bila je metodom imunohistokemijskih bojenja locirati dendritičke stanice, te dio imunokompetentnog sustava dentalne pulpe u histološkim uzorcima normalne i ireverzibilno inflamirane ljudske zubne pulpe. Materijal i metode: Zbog posebnih svojstava vezivnog tkiva ljudske zubne pulpe, koristila su se sljedeća antitijela: vimentin V9, protein povezan s neurofi lamentima (NFAP), protein S 100 te aktin glatkih mišića (klon 1A4). Rezultati: Rezultati su pokazali da humana dentalna pulpa ima mnogobrojne stanice s razgranatim citoplazmatskim produžecima u području upalnoga infiltrata. Na temelju imunohistokemijskog bojenja smatramo da je riječ o dendritičkim stanicama. Zaključak: Dendritičke stanice u upaljenoj ljudskoj zubnoj pulpi nisu neočekivane s obzirom na veliku gustoću upalnog infiltrata i ulogu koju imaju u upalnom odgovoru. Podtip i značenje tih stanica treba još objasniti.Objectives: The aim of this study was to locate the dendritic cells, a part of the immunocompetent system of dental pulp, in histological samples of normal and irreversibly inflamed human dental pulp, using immunohistochemical staining methods. Material and Methods: In light of the particular character of the connective tissue of human dental pulp, the following antibodies were used: vimentin clone V9, a protein associated with neurofilaments (NFAP), the S 100 protein, and smooth muscle type actin (clone 1A4). Results: The results indicated that human dental pulp has numerous cells with branching cytoplasmic extensions, in areas of inflammatory infiltrate. Based on immunohistochemical staining we consider these to be dendritic cells. Conclusions: The presence of dendritic cells in inflamed human dental pulp is not unexpected, considering the great density of the inflammatory infiltrate, and the role they play in the inflammatory response. The subtype and significance of these cells need to be clarified

Seasonal variation in nutrient content of some leafy vegetables from Banat County, Romania

The aim of this study is to investigate the effect of seasonal variation of the moisture content and trace metal composition of spinach, butter lettuce and parsley leaves. The content of bioactive compounds in green leafy vegetables at harvest has beneficial effects on human health. It was found that moisture, macro and microelement content vary from one season to another. Water content measured by thermo-gravimetric method is presenting small variation during the seasonal change, revealing the lowest value for spinach, butter lettuce and parsley leaves, in June. Calcium, magnesium and potassium concentrations in leaves are decreasing in spinach, lettuce and parsley from April to September, but the variations are small. Copper is accumulating mostly in parsley leaves and less in lettuce and spinach. Knowing the concentration of important nutrients in different seasons helps do decide the harvesting period in according to the richness of the diet for each individual

The impact of agricultural performance on foreign trade concentration and competitiveness: empirical evidence from Romanian agriculture

Analysing the impact of agricultural performance on foreign trade concentration and competitiveness defines an important step in identifying the opportunities, challenges and proactive measures in designing a functional and marked based agricultural model. The scope of this study is to investigate the evolution of Romania’s the foreign trade competitiveness and its concentration on main destinations during 2007–2016. In this context, in the paper are identified and investigated some of the competitiveness’ mutations arisen from the Romania’s trade flows concentration and restructuration in relation with 26 of the European Union member states. It was used a unique dataset on agro -food trade output and concentration across EU countries to construct measures of trade competitiveness. The results obtained suggest the existence of a dual relationship of the Romanian agriculture competitiveness and its dependence on the EU economic area, by the concentration of the commercial relationships

Statistical evaluation of heavy metal content in some capsicum varieties available on the Romanian market

The aim of this study is to emphasis the heavy metals content in seven capsicum varieties of capsicum of the specie Capsicum annuum L., available on the Romanian market. From the analyzed Capsicum fruit samples, three Capsicum assortments were cultivated in Romania and the other four were imported from Italy and Turkey. The studied heavy metals (copper, zinc, manganese, iron, cobalt, lead, nickel, cadmium and chromium) have normal concentration values that are not of any risk to human health. Cadmium is not detectable in all studied samples. The heavy metal content associated with statistical analysis programs permits the identification of characteristics specific to the origin of products and the graphical chemical fingerprint of the studied capsicum species. The chemical fingerprinting of a plant demands the determination of a large number of elements (DJINGOVA ET AL., 2004). The study is revealing similar distribution pattern

Graphical chemical fingerprints of parsley, dill and lovage leaves

The aim of this study is to emphasis the use of thermo gravimetrical water content and trace metals analysis to identify the chemical graphical fingerprints of parsley, dill and lovage leaves. Copper, zinc, manganese, iron, nickel and lead have normal concentration values that are not of any risk to human health. Cobalt, chromium and cadmium were not detectable in all studied samples. The water and present trace metals contents associated with mathematical models permits the identification of characteristics specific to the studied vegetable leaves as well as the graphical chemical fingerprints. The study is revealing similar distribution pattern

Changes in the structure of actinomycete populations in the rhizosphere of vicia sativa species

It is a known fact that species of legumes improve the soil they are grown on, but at the same time, they produce the so-called rhizosphere effect or rhizodeposit that has a selective effect on the microorganisms which are considered "fertility effectors" for soil. From the three studied area the highest number of actinomycetes was found in edaphosphere and the lowest number in the area influenced by roots. Among the few factors under research for the purpose of this paper, humus and potassium were observed to have the strongest impact on this group. Humidity is a factor that could change the competition between soil microorganisms and plants in the soil for N and it could affect the stability of aggregates

Vegetables, fruits, honey and pollen, a natural source of zinc

This work aimed to evaluate zinc content in six common vegetables (parsley, carrot, dill, onion, cucumber and beans), three common fruits (apples, raspberry and dog rose), bees honey and pollen. Determination of zinc content in soil, raw vegetables and fruits, as well as in bees honey and pollen from local apiaries were carried out by FAAS. All experiments and analyses were performed in triplicate. Overall, the highest zinc concentration was found in the leaves (carrot 48.07 mg kg-1, parsley 46.62 mg kg-1, dog rose leaves 36.13 mg kg-1) and beans crops (41.26 mg kg-1). According to our study the highest content occurs in the plants of the Apiaceae Family and in Rosa canina leaves

Seasonal variation in nutrient content of some leafy vegetables from Banat County, Romania

The aim of this study is to investigate the effect of seasonal variation of the moisture content and trace metal composition of spinach, butter lettuce and parsley leaves. The content of bioactive compounds in green leafy vegetables at harvest has beneficial effects on human health. It was found that moisture, macro and microelement content vary from one season to another. Water content measured by thermo-gravimetric method is presenting small variation during the seasonal change, revealing the lowest value for spinach, butter lettuce and parsley leaves, in June. Calcium, magnesium and potassium concentrations in leaves are decreasing in spinach, lettuce and parsley from April to September, but the variations are small. Copper is accumulating mostly in parsley leaves and less in lettuce and spinach. Knowing the concentration of important nutrients in different seasons helps do decide the harvesting period in according to the richness of the diet for each individual

Statistical evaluation of heavy metal content in some capsicum varieties available on the Romanian market

The aim of this study is to emphasis the heavy metals content in seven capsicum varieties of capsicum of the specie Capsicum annuum L., available on the Romanian market. From the analyzed Capsicum fruit samples, three Capsicum assortments were cultivated in Romania and the other four were imported from Italy and Turkey. The studied heavy metals (copper, zinc, manganese, iron, cobalt, lead, nickel, cadmium and chromium) have normal concentration values that are not of any risk to human health. Cadmium is not detectable in all studied samples. The heavy metal content associated with statistical analysis programs permits the identification of characteristics specific to the origin of products and the graphical chemical fingerprint of the studied capsicum species. The chemical fingerprinting of a plant demands the determination of a large number of elements (DJINGOVA ET AL., 2004). The study is revealing similar distribution pattern