An optimized HRM method for diagnosis of G6PD deficiency in kinh Vietnamese via viangchan mutation

With Glucose-6-phosphate dehydrogenase (G6PD) deficiency being the most common enzyme disorder in human, there have been 184 discovered point mutations and several methods that have been applied for diagnosing this disease. However, these techniques often pose several major problems such as being time-consuming, low sensitivity and high cost. Recently, the High Resolution Melting (HRM) has been studied and proven to be effective for DNA genotyping, mutation scanning and sequence matching. Therefore, HRM has been chosen for diagnosing G6PD deficiency via Viangchan mutation in this study. In this study, a total of 56 dried blood spot samples (including six control samples which were known the exact genotype by sequencing and fifty unknown samples) were collected and extracted DNA by using QIAamp DNA Blood Mini Kit. Primers for HRM analysis were designed through by the Umelt software. Then HRM optimization was carried out for annealing temperature of primers (Ta) and MgCl2 concentration on six control samples. The optimized HRM protocol with 2.5 μM of MgCl2 and Ta at 62oC was applied for fifty G6PD samples and then comparing with ARMS-PCR genotyping results for the validation process. In the final step, genotyping results were confirmed by sequencing. In a results, both sensitivity and specificity of this technique reached 100%. Based on these favorable outcomes, this study has successfully optimized the HRM conditions for diagnosing fifty G6PD samples. It was such an essential precondition that showed HRM could be applied for other types of G6PD through other types of mutations such as Canton mutation or continues to be developed for HRM-Multiplex reactions

Impact of trained human resources, adoption of technology and international standards on the improvement of accounting and auditing activities in the agricultural sector in Viet Nam

Recently, the adoption of accounting and auditing standards in the agricultural sector has been a global phenomenon that has gained increasing trend due to the significant role of the agricultural industry in the country's economy. Thus, the present study examines the impact of trained human resources, technology adoption, and international standards on improving accounting and auditing activities in the agricultural sector in Vietnam. The present research investigates the moderating impact of agricultural, institutional support among the linkage of trained human resources, technology adoption, international standards, and improvement of accounting and auditing activities in the agricultural sector. The present article has adopted the questionnaires to gather the primary data from selected respondents. The current research has applied the smart-PLS to test the study's hypotheses. The results revealed that trained human resources, technology adoption, and international standards positively impact the improvement of accounting and auditing activities in the agricultural sector. The findings also indicated that the agricultural institutional support significantly moderates the linkage of trained human resources, technology adoption, international standards, and improved accounting and auditing activities in the agricultural sector.Nguyen Van Hoa (University of Kinh Bac (UKB)), Nguyen Thi Hanh Duyen (Vinh University), Vu Ngoc Huyen (Viet Nam National University of Agriculture (VNUA)), Hoang Vu Quang (Researcher at Institute of Policy and Strategy for Agriculture and Rural Development (IPSARD)), Nguyen Van Huong (Hung Yen University of Technology and Education (UTEHY)), Nguyen Thi Cam Tu (National Economics University (NEU)), Bui Thi Minh Nguyet (Vietnam National University of Forestry (VNUF))Includes bibliographical references

EFFECT OF SOME DISPERSING AUXILIARIES ON NANOSILICA DISPERSION INTO PASSIVE CHROME TRIVALENT SOLUTION

In this paper, the dispersion of nanosilica in conversion solution trivalent chromium using variety of dispersion aids namely: SDS, PVP, AE-7, OP-10 and epomin. The results showed that the nanosilica could disperse in conversion solution in low pH (pH = 1.5). The solution containing nanosilica was uniform, having no agglomeration with SDS, PVP and AE-7 after 7-day preparation. The results of zeta potential and size distribution illustrated that AE-7 was the most effective for nanosilica dispersion in passive solution trivalent chromium with medium size of nanosilica in C2-nanosilica solution using AE-7 equal ~ 60 nm. However, there was a much diffirence size between the size of nanosilica in passive solution and initial nanosilica. As a result, nanosilica could disperse in passive solution at low pH with AE-7 but this was not effective enough to held dispersed solution in stable state

Updated upper limit of normal for serum alanine aminotransferase value in Vietnamese population

Background: Alanine aminotransferase (ALT) is a marker of hepatic damage and its range can be affected by viral hepatitis, alcoholic hepatitis and non-alcoholic fatty liver diseases. We aimed to study the factors associated with higher ALT level and update the upper limit of normal (ULN) in the Vietnamese population.Methods: This cross-sectional study enrolled 8383 adults, aged 18 years and older who visited the Medical Center at Ho Chi Minh City for a health check-up. Following the exclusion criteria, 6677 subjects were included in the analysis.Results: Age ≤40 years, male gender, body mass index >23 kg/m2, diastolic blood pressure >85 mm Hg, cholesterol >5.2 mmol/L, triglyceride >1.7 mmol/L, positivity, anti-hepatitis C virus positivity and fatty liver (p40 U/L). Without considering age and gender, healthy group is defined after exclusion of participants with one of the mentioned contributing factors. The median ALT level in the healthy group was 18 in men and 13 in women. The ULN at the 95th percentile of the healthy group was 40 U/L in men and 28 U/L in women.Conclusion: The ULN for ALT in healthy women was lower than in healthy men. Updated ULN for ALT level can promote the identification of unhealthy subjects. More studies that involve ethnicity and lifestyle factors are needed to confirm the new ULN in the Vietnamese population

The effect of environmental accounting information disclosure on financial performance of Vietnamese listed industrial firms: The moderating role of Leverage and Big4

Environmental Accounting Information Disclosure (EAID) is the most recent novelty in corporate non-financial information reporting practice. This study aims to evaluate the effects of EAID on the financial performance of Vietnamese enterprises. The population of this study comprises listed companies in the industrial sector on the Ho Chi Minh Stock Exchange for the period of five years (2017–2021). The research employed a purposive sampling technique and some analysis techniques, such as content analysis and regression analysis, including Ordinary Least Squares, Fixed Effects Model, Random Effects Model, and Feasible General Least Squares. The final regression results show a positive effect of EAID on profitability. Specifically, as a moderating variable, leverage weakens the relationship when financial performance is measured by ROA and ROE, whereas the quality of financial statements is assured by the Big 4, which strengthens the nexus between EAID and ROE. The study cannot confirm the negative moderating variable of the duality of the CEO position in the relationship. The research gives implications for improving financial performance with increased EAID and some future research directions

Adsorption of ciprofloxacin using composite Film from PVA, agarose and maltodextrin

Rintangan antibiotik ialah salah satu masalah yang paling membimbangkan hari ini. Oleh itu, membran komposit telah digunakan secara meluas untuk penyingkiran antibiotik daripada air. Filem PVA/Agarosa/Maltodekstrin telah disintesis dengan tuangan dengan pelbagai nisbah komponen. Ia dinilai untuk ciri melalui kelembapan, keterlarutan, pengembangan dan keputusan BET. Keputusan menunjukkan bahawa filem PVA/Agarosa/Maltodekstrin mempamerkan daya maju terbaik dalam persekitaran akuatik melalui keterlarutan rendah (68.88% ± 0.03), bengkak tinggi (431.77% ± 5.89) dan isi padu liang (0.034969 cm³/g). Kapasiti penjerapan PVA/Agarosa/Maltodekstrin telah diuji untuk beberapa antibiotik seperti Ciprofloksacin, Tetracycline, Oxy-Tetracycline dan Chloramphenicol. Keputusan menunjukkan bahawa Ciprofloksacin telah dikeluarkan oleh filem PVA/Agarosa/Maltodekstrin lebih baik daripada antibiotik lain. Penjerapan antibiotik tertinggi diperoleh pada 20 minit, suhu 30 °C, dos 2 g/L, pH 6 dan kepekatan antibiotik 40 mg/L. Penjerapan Ciprofloksacin telah diramalkan melalui model kinetik dan isoterma penjerapan. Keserasian kinetik Pseudo First Order dan isoterma Dubinin-Radushkevich telah menunjukkan bahawa penjerapan berlaku mengikut mekanisme penjerapan fizikal dengan interaksi elektrostatik pada permukaan bahan. Kapasiti penjerapan maksimum direkodkan pada 4.48 mg/g berdasarkan isoterma Dubinin-Radushkevich

Characteristics of Antibiotic-resistant E. coli Isolated from Perfume River in Hue City, Vietnam

Antibiotic resistant bacteria (ARB) in aquatic environments is attracting increasing attention. However, the spread of ARB along Perfume River in Hue City is poorly understood. This study aimed to phenotypically and genotypically characterize β-lactam- and fluoroquinolone-resistant Escherichia coli isolates from this river. Water samples were collected from the urban, rural, agricultural, and less-affected areas in March 2020. E. coli susceptibility to seven commonly employed antibiotics was analyzed using the disk diffusion method, and the antibiotic resistance genes (ARGs), qnrA, qnrB, qnrS, TEM, SHV, and CTX-M, were identified using polymerase chain reaction (PCR) and DNA sequencing. The antibiotic susceptibility patterns of E. coli revealed that the rate of amoxicillin resistance was the highest (60%). PCR assays and sequencing of 12 β-lactam-resistant E. coli isolates indicated the presence of blaTEM and blaCTX-M-15 in 58.3% and 16.7% of the isolates, respectively. Only one of four fluoroquinolon -resistant E. coli isolates harbored the qnrS, while qnrA or qnrB genes were not detected. These findings suggest that this water may be an essential source of transmissible ARGs in Hue City, which may have a detrimental impact on the people living in this area

Lovastatin for the Treatment of Adult Patients With Dengue: A Randomized, Double-Blind, Placebo-Controlled Trial.

BACKGROUND: Dengue endangers billions of people in the tropical world, yet no therapeutic is currently available. In part, the severe manifestations of dengue reflect inflammatory processes affecting the vascular endothelium. In addition to lipid lowering, statins have pleiotropic effects that improve endothelial function, and epidemiological studies suggest that outcomes from a range of acute inflammatory syndromes are improved in patients already on statin therapy. METHODS: Following satisfactory review of a short pilot phase (40 mg lovastatin vs placebo in 30 cases), we performed a randomized, double-blind, placebo-controlled trial of 5 days of 80 mg lovastatin vs placebo in 300 Vietnamese adults with a positive dengue NS1 rapid test presenting within 72 hours of fever onset. The primary outcome was safety. Secondary outcomes included comparisons of disease progression rates, fever clearance times, and measures of plasma viremia and quality of life between the treatment arms. RESULTS: Adverse events occurred with similar frequency in both groups (97/151 [64%] placebo vs 82/149 [55%] lovastatin; P = .13), and were in keeping with the characteristic clinical and laboratory features of acute dengue. We also observed no difference in serious adverse events or any of the secondary outcome measures. CONCLUSIONS: We found lovastatin to be safe and well tolerated in adults with dengue. However, although the study was not powered to address efficacy, we found no evidence of a beneficial effect on any of the clinical manifestations or on dengue viremia. Continuing established statin therapy in patients who develop dengue is safe.Chinese Clinical Trials Registration. ISRCTN03147572

Primary Care Influenza-like Illness Surveillance in Ho Chi Minh City, Vietnam 2013-2015

BACKGROUND: Year-round transmission of influenza has been detected in Vietnam through both national surveillance and other epidemiological studies. Understanding the demographic and clinical features of influenza-like-illness (ILI) presenting to primary care in urban Vietnam is vital to understand these transmission dynamics. METHODS: A prospective, observational study of patients with ILI in Ho Chi Minh City, Vietnam was conducted between August 2013 and November 2015 in a mix of public and private primary care settings. Molecular testing for Influenza A & B and 12 other respiratory viruses was performed. RESULTS: 1152 ILI patients were recruited. 322 and 136 subjects tested positive for influenza A and B, respectively. 193 subjects tested positive for another respiratory virus; most commonly rhinovirus and parainfluenza virus 3. Influenza was detected in 81% of the 116 study weeks. Three peaks of influenza activity were detected; an H3N2 peak April-June 2014, an influenza B peak July-December 2014, and a mixed H3N2 and H1N1 peak March-September 2015. Subjects recruited from private clinics were more likely to have higher income, and to have reported previous influenza vaccination. Antibiotic use was common (50.3%) despite limited evidence of bacterial infection. CONCLUSION: Influenza in southern Vietnam has complex transmission dynamics including periods of intense influenza activity of alternating types and subtypes. Broadening surveillance from hospital to the community in tropical settings is feasible and a valuable for improving our understanding of the global spread and evolution of the virus. This article is protected by copyright. All rights reserved