POPULATION GENETIC DIVERSITY OF CAMELLIA DILINHENSIS ON THE DI LINH PLATEAU OF VIETNAM REVEALED BY ISSR AND SCOT MARKERS

Genetic diversity of naturally distributed Camellia dilinhensis populations on the Di Linh plateau of Vietnam was assessed by the inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) techniques separately and then by combining data from both techniques for satisfactory results. The genetic diversity parameters and genetic distances among individuals found with the ISSR technique (HeI = 0.1420, II = 0.2092, PPBI = 35.22%, GSCsI = 0.765–0.988, and AGSCI = 0.915) are lower than those found with the SCoT technique (HeS = 0.2100, IS = 0.381, PPBS = 52.27%, GSCsS = 0.644–0.985, and AGSCS = 0.866). Based on the combined data from both techniques, the level of genetic diversity of the investigated population is PPB = 43.77%, He = 0.1720, I = 0.2582, and the genetic similarities among individuals are GSCs = 0.764–0.973 with an average of AGSC = 0.894. The SCoT technique differentiated between individuals better and reflected a higher level of genetic diversity in the population than the ISSR technique, but the ISSR technique revealed more loci in Camellia dilinhensis plants than did the SCoT technique

LECTIN THỰC VẬT VÀ TIỀM NĂNG ỨNG DỤNG TRONG KIỂM SOÁT CÔN TRÙNG GÂY HẠI

Phytophagous insects is one of the major biological constraints in crop production. To cope with continuous threat from insects, plants produce insecticidal peptides or proteins. Plant lectins are carbohydrate binding proteins, one of the most important secondary metabolites which also serve as a defense tool against plant-eating organisms. Although most lectins have moderate effects to development, fecundity or growth of insect, several lectins are highly toxic to insects. In the last decade, many studies reported a role of lectins in pest management. In particular, several plant lectins respond to different kinds of stress such as drought, fire, wounding, high-salinity, hormone treatment and pathogen attack. This group of lectin is called as “inducible plants lectin”. This review summarized recent progresses in research on the application of lectin as a potential approach for integrated pest management. Côn trùng gây hại được biết đến như là yếu tố sinh học bất lợi gây ảnh hưởng trực tiếp đến sinh trưởng và phát triển của cây nông nghiệp. Thực vật tự bảo vệ bằng cách tổng hợp ra protein hay peptide đối kháng, trong đó lectin được xem như một nhóm protein gây độc hiệu quả đối với côn trùng. Hầu hết các lectin chỉ ảnh hưởng ở mức độ vừa phải đến sự sinh trưởng và phát triển nhưng một số lại có khả năng dẫn đến tỷ lệ tử vong cao đối với côn trùng. Trong  một thập kỷ trở lại, rất nhiều nghiên cứu đã được công bố liên quan đến khả năng kháng côn trùng hiệu quả của một số loại lectin trên cây trồng, đặc biệt là chúng có thể được cảm ứng sinh tổng hợp trong các điều kiện môi trường bất lợi. Bài viết này tóm tắt và thảo luận các thành tựu nghiên cứu về tiềm năng ứng dụng của lectin trong quản lý dịch hại trên đồng ruộng. Đây được coi là hướng nghiên cứu tiềm năng cho ngành trồng trọt, nhất là trong bối cảnh các điều kiện bất lợi gây ra bởi biến đổi khí hậu ngày càng ảnh hưởng nghiêm trọng đến tình hình sản xuất cây nông nghiệp như hiện nay

Genetic diversity of Panax stipuleanatus Tsai in North Vietnam detected by inter simple sequence repeat (ISSR) markers

Panax stipuleanatus Tsai is a type of medicinal plant within north-west Vietnam. In this study, inter simple sequence repeat markers were employed to investigate the genetic diversity of naturally distributed populations classified by habitat for this species. Genetic diversity at the species level was moderate (HeT = 0.254; PPBT = 96.02%). Genetic diversity was not equal in two populations. The result showed higher population genetic diversity in the Lao Cai region (HeBX = 0.266; PPBBX = 91.48%) as compared to those located in Lai Chau region (HeHT = 0.235; PPBHT = 84.66%). The interpopulation gene differentiation was small (GSTP = 0.03) with the genetic distance among populations was DP = 0.0103. Gene flow within populations was as high as Nm = 7.36

Synthesis, molecular docking analysis and in vitro evaluation of new heterocyclic hybrids of 4-aza-podophyllotoxin as potent cytotoxic agents

International audienceTwo different synthetic approaches to novel heterocyclic hybrid compounds of 4-azapodophyllotoxin were investigated. The obtained products were characterized by infrared spectroscopy, nuclear magnetic resonance spectroscopy, and high-resolution mass spectrometry. MTT protocol was then performed to examine the cytotoxic activity of these products against KB, HepG2, A549, MCF7, and Hek-293 cell lines. The cytotoxic assessment indicated that all products displayed moderate to high cytotoxicity against all tested cancer cell lines. The most active compound 13k containing the 2-methoxypyridin-4-yl group exhibited selective cytotoxicity against KB, A549, and HepG2 cell lines with the IC50 values ranging from 0.23 to 0.27 mu M, which were between 5- to 10-fold more potent than the positive control ellipticine. Compounds 13a (HetAr = thiophen-3-yl) and 13d (HetAr = 5-bromofuran-2-yl) displayed high cytotoxic selectivity for A549 and HepG2 cancer cell lines when compared to the other cancer cell lines and low toxicity to the normal Hek-293 cell line. Molecular docking study was conducted to evaluate the interaction of new synthesized compounds with the colchicine-binding-site of tubulin. Besides that, physicochemical and pharmacokinetic properties of the most active compounds 13h,k were predicted