Determination of maximum allowable concentration and LC50 96h of Sefidroud River sediments for Persian sturgeon (Acipenser persicus) fingerlings

The impact of Sefidroud River sediments on the fingerlings of Persian Sturgeon (Acipenser persicus) was studied in 2005. The tests were done in 20 liter aquariums each containing 10 Persian Sturgeons fingerlings weighing 3-5 grams each. We devised 6 treatments and a control with 3 repetitions in the four day investigation through which the lethal concentration (LC50 96h) of Sefidroud sediments were studied. During the test, physicochemical parameters of water such as pH, oxygen and temperature were measured as 8, 8.5mg/1 and 25±1°C respectively. The results showed that LC5096h and LC50 24h of sediments on Persian sturgeon were 15367.39mg/1 and 124882.04mg/1 respectively. We determined the maximum allow-able concentration (M.A.C) of sediments to be I 536.74mg/l

SMOTEC: An Edge Computing Testbed for Adaptive Smart Mobility Experimentation

Smart mobility becomes paramount for meeting net-zero targets. However, autonomous, self-driving and electric vehicles require more than ever before an efficient, resilient and trustworthy computational offloading backbone that expands throughout the edge-to-cloud continuum. Utilizing on-demand heterogeneous computational resources for smart mobility is challenging and often cost-ineffective. This paper introduces SMOTEC, a novel open-source testbed for adaptive smart mobility experimentation with edge computing. SMOTEC provides for the first time a modular end-to-end instrumentation for prototyping and optimizing placement of intelligence services on edge devices such as augmented reality and real-time traffic monitoring. SMOTEC supports a plug-and-play Docker container integration of the SUMO simulator for urban mobility, Raspberry Pi edge devices communicating via ZeroMQ and EPOS for an AI-based decentralized load balancing across edge-to-cloud. All components are orchestrated by the K3s lightweight Kubernetes. A proof-of-concept of self-optimized service placements for traffic monitoring from Munich demonstrates in practice the applicability and cost-effectiveness of SMOTEC

Decentralized Edge-to-Cloud Load-balancing: Service Placement for the Internet of Things.

The Internet of Things (IoT) requires a new processing paradigm that inherits the scalability of the cloud while minimizing network latency using resources closer to the network edge. On the one hand, building up such flexibility within the edge-to-cloud continuum consisting of a distributed networked ecosystem of heterogeneous computing resources is challenging. On the other hand, IoT traffic dynamics and the rising demand for low-latency services foster the need for minimizing the response time and a balanced service placement. Load-balancing for fog computing becomes a cornerstone for cost-effective system management and operations. This paper studies two optimization objectives and formulates a decentralized load-balancing problem for IoT service placement: (global) IoT workload balance and (local) quality of service (QoS), in terms of minimizing the cost of deadline violation, service deployment, and unhosted services. The proposed solution, EPOS Fog, introduces a decentralized multi-agent system for collective learning that utilizes edge-to-cloud nodes to jointly balance the input workload across the network and minimize the costs involved in service execution. The agents locally generate possible assignments of requests to resources and then cooperatively select an assignment such that their combination maximizes edge utilization while minimizes service execution cost. Extensive experimental evaluation with realistic Google cluster workloads on various networks demonstrates the superior performance of EPOS Fog in terms of workload balance and QoS, compared to approaches such as First Fit and exclusively Cloud-based. The results confirm that EPOS Fog reduces service execution delay up to 25% and the load-balance of network nodes up to 90%. The findings also demonstrate how distributed computational resources on the edge can be utilized more cost-effectively by harvesting collective intelligence

Determination of LC_(50) 96h of dyazinon, hinosan and tilt for Acipenser nudiventris fingerlings

The toxic effects of agricultural pesticides Dyazinon, Hinosan and Tilt (Propiconazole) were studied on Acipenser nudiventris fingerlings (1-3g) in Spring 2005. We conducted our experiments in aquaria holding 20L water in static systems based on O.E.C.D method using five treatments, one control concentration and three replications. In each aquarium, ten individual Ship Sturgeon fingerlings were introduced, and water physical and chemical factors were controlled to be in the range pH=7-8, TH>400mg/l (CaCO _(3)) as for the water used for culture, and DO=80% and temperature was kept between 24 ±1°C. Our results for the LC _(50) value of Dyazinon, Hinosan and Tilt in 96 hours were 0.36, 0.28 and 3.9mg/l in the fish fingerlings. We also determined the maximum allowable concentration value of the toxins at 0.036, 0.028 and 0.39, respectively

Effect of dietary honey on intestinal microflora and toxicity of mycotoxins in mice

BACKGROUND: Bee honey is a functional food which has a unique composition, antimicrobial properties and bifidogenic effect. In order to assess whether honey can inhibit the toxic effect of mycotoxins, the present study was undertaken. METHODS: Production of biomass and toxins by Aspergillus parasiticus and Aspergillus ochraceus were followed in media without and with honey. Although aflatoxins and ochratoxin A. were administrated to male Swiss albino mice up to 1 μg and 10 ng/kg body weight/day respectively. The experimental animals were fed diets without our with 10% honey for two months. The changes in colonic probiotic bacteria, determintal colon enzyme glucuronidases, and genotoxicity were followed. RESULTS: Addition of 32% in its media increased the biomass of A parasiticus, while the biomass of A. ochraceus decreased and Ochratoxin A. was not produced. When the honey was added at the ratio of 32 and 48% in the medium. No relationship was found between mycelium weight and production of mycotoxins. Oral administration of aflatoxins (mixture of B(1), B(2), G(1) and G(2)) and Ochratoxin A. induced structural and numerical chromosomal aberrations in bone marrow and germ cells of male mice, whereas, honey treatment reduced the genotoxicity of mycotoxins. Also both toxins induced histopathological changes in liver and kidney. Feeding on diet supplemented with honey improved the histopathological changes in case of aflatoxin group, but not in the case of ochratoxin A. group (except of kidney in two cases). No significant differences were found in the activity of colon β-glucuronidase between group fed diet with or without honey. On the other hand, the colon bifido bacteria and lactobacilli counts were increased markedly in group receiving diet supplemented with honey. CONCLUSION: Substituting sugars with honey in processed food can inhibit the harmful and genotoxic effects of mycotoxins, and improve the gut microflora

Bacteriophage biodistribution and infectivity from honeybee to bee larvae using a T7 phage model

Bacteriophages (phages) or viruses that specifically infect bacteria have widely been studied as biocontrol agents against animal and plant bacterial diseases. They offer many advantages compared to antibiotics. The American Foulbrood (AFB) is a bacterial disease affecting honeybee larvae caused by Paenibacillus larvae. Phages can be very significant in fighting it mostly due to European restrictions to the use of antibiotics in beekeeping. New phages able to control P. larvae in hives have already been reported with satisfactory results. However, the efficacy and feasibility of administering phages indirectly to larvae through their adult workers only by providing phages in bees feeders has never been evaluated. This strategy is considered herein the most feasible as far as hive management is concerned. This in vivo study investigated the ability of a phage to reach larvae in an infective state after oral administration to honeybees. The screening (by direct PFU count) and quantification (by quantitative PCR) of the phage in bee organs and in larvae after ingestion allowed us to conclude that despite 104 phages reaching larvae only an average of 32 were available to control the spread of the disease. The fast inactivation of many phages in royal jelly could compromise this therapeutic approach. The protection of phages from hive-derived conditions should be thus considered in further developments for AFB treatment.This study was supported by the project APILYSE, PTDC/CVT-EPI/4008/2014 - POCI-01-0145-FEDER-016598, - funded by FEDER through COMPETE 2020 - Programa Operacional Competitividade e Internacionalização (POCI) and by national funds trough FCT - Fundação para a Ciência e a Tecnologia, I.P. The work was also supported by the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004), funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. HR was supported by FCT through the grant SFRH/BD/128859/2017. RC was founded by FCT and FEDER (POCI-010145-FEDER-007274).info:eu-repo/semantics/publishedVersio

Crystal Structure of the Formin mDia1 in Autoinhibited Conformation

Formin proteins utilize a conserved formin homology 2 (FH2) domain to nucleate new actin filaments. In mammalian diaphanous-related formins (DRFs) the FH2 domain is inhibited through an unknown mechanism by intramolecular binding of the diaphanous autoinhibitory domain (DAD) and the diaphanous inhibitory domain (DID).Here we report the crystal structure of a complex between DID and FH2-DAD fragments of the mammalian DRF, mDia1 (mammalian diaphanous 1 also called Drf1 or p140mDia). The structure shows a tetrameric configuration (4 FH2 + 4 DID) in which the actin-binding sites on the FH2 domain are sterically occluded. However biochemical data suggest the full-length mDia1 is a dimer in solution (2 FH2 + 2 DID). Based on the crystal structure, we have generated possible dimer models and found that architectures of all of these models are incompatible with binding to actin filament but not to actin monomer. Furthermore, we show that the minimal functional monomeric unit in the FH2 domain, termed the bridge element, can be inhibited by isolated monomeric DID. NMR data on the bridge-DID system revealed that at least one of the two actin-binding sites on the bridge element is accessible to actin monomer in the inhibited state.Our findings suggest that autoinhibition in the native DRF dimer involves steric hindrance with the actin filament. Although the structure of a full-length DRF would be required for clarification of the presented models, our work here provides the first structural insights into the mechanism of the DRF autoinhibition