Quantitative Determination of Norepinephrine by HPLC in Rodent Urine Sample

Abstract Norepinephrine, a key neurotransmitter that has been linked to a variety of neuropsychiatric diseases. However, there is limited work on employing HPLC-UV to estimate monoamines in biological samples using HPLC-UV method. The present study explores the detection of norepinephrine in rat urine sample, developing a easy, precise validated HPLC method. The o-phosphoric acid (80%):acetonitrile (70:30) combination was used as the mobile phase, and a flow rate of 0.5 ml/min was used to produce the chromatographic separation on a C8 column (250 x 4.6 mm, 5 m). The detection was observed at λmax 275 nm with better sensitivity. According to the parameters indicated in the ICH guidelines (Q2A; Q2B), the procedure was verified. The linearity range was selected from 10-35 µg/mL, r2 =0.966, LOD (1.17 µg/mL), LOQ (3.55 µg/mL), ret. time (4 min). The technique has demonstrated that it is repeatable and recoverable within the given range. Thus can be used for routine analysis of norepinephrine in urine samples

Development and Validation of Analytical Method for Estimation of Berberine in Herbal Extract of Momordica Dioica

Background:While several anti-diabetic medications have been found to lower blood glucose levels, there are numerous negative effects connected with the use of currently marketed anti-diabetic medications.However, there are medicinal plants with anti-diabetic effects with low or no side effects. Among the Cucurbitaceae family of plants, Momordica dioica is a dioecious climbing herb. It contains many phytoconstituents.One of it is a quaternary ammonium salt of benzyl-isoquinoline alkaloid called as berberine exhibiting a wide variety of pharmaceutical properties.In the current investigation, the activities of berberine for its anti-diabetic potential in the Momorica Dioica plant.The goal of the recent effort is to establish and validate an HPTLC method that is quick, accurate, exact, and specific for determining berberine from herbal extract of Momordica dioica. Methodology/Conclusions/significance: For a quick examination to determine the amount of berberine, a (HPTLC) process was created as well as validated.On HPTLC aluminium plate 60 F254,precoated with silica gel  chromatographic separation was accomplished using the solvents: methanol ,ethyl acetate, toluene and formic acid (2:4:3:0.5). A wavelength of 348 nm was used for detection. Rf was found to be 0.44±5% for berberine. There was linearity for berberine in the 400ng/band concentration range.The LOD and LOQ was found to be0.0096ng/band and 0.0293ng/ band for Berberine.The average berberine recovery rate was (0.041).The method's precision, accuracy, linearity, robustness, and specificity were all authenticated in accordance with ICH guidelines. The discovered approach can be used to regularly analyse Berberine in herbal formulations for quality control

Evaluation of Anti-Inflammatory and Analgesic Property of Methanolic Extract of Momordica Dioica in Wistar Rat Model

Background: Since current medications frequently cause potentially serious side effects, countless individuals continued to have pain and inflammation despite the accessibility of treatments. Ancient practitioners have used a variety of herbal remedies with analgesic and anti-inflammatory properties extensively. Momordica Dioica is one of them, but no experimental research has been done in support of this traditional use.  Objective: The purpose of the research was to assess the analgesic and anti-inflammatory properties of methanolic Momordica Dioica fruit extracts. Methods: Dryed by air According to OECD guideline the version eighteen, the fruit of Momordica Dioica is extracted with the solvent methanol as well as an acute oral toxicity study was done for the methanol-based extract of Momordica Dioica. The Active Constituent responsible for the Activity was confirmed by HPTLC method. The hot plate method was employed to assess the analgesic efficacy of centrally mediated analgesia.The ability to reduce inflammation was assessed using a carrageenan-induced paw oedema test. Results: Momordica dioica's methanolic extract inhibits the ceiling result during activity.  The Methanolic Extract of Momordica dioica (MEMD) extract demonstrated a significant (p 0.0001) analgesic effect in the hot plate method at doses of 100mg/kg and in all combinations with standard. Maximum anti-inflammatory effects were seen in carrageenan-induced paw edema starting 2-4 hours after induction, and all tested doses of the extract significantly inhibited the carrageenan-induced inflammation (p 0.0001, p 0.001). The presence of a pentacyclic terpenoid i.e Ursolic acid present in the extract had potential analgesic and anti-inflammatory activity which supports the traditional claim