1,943 research outputs found

    Broad and Branch Mixed Use Development

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    Seed production of barnyardgrass (Echinochloa crus-galli) in response to time of emergence in cotton and rice

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    The spread of herbicide resistance in barnyardgrass (Echinochloa crus-galli (L.) Beauv.) poses a serious threat to crop production in the southern United States. A thorough knowledge of the biology of barnyardgrass is fundamental for designing effective resistance-management programmes. In the present study, seed production of barnyardgrass in response to time of emergence was investigated in cotton and rice, respectively, in Fayetteville and Rohwer, Arkansas, over a 2-year period (2008–09). Barnyardgrass seed production was greater when seedlings emerged with the crop, but some seed production was observed even if seedlings emerged several weeks after crop emergence. Moreover, barnyardgrass seed production was highly variable across environments. When emerging with the crop (0 weeks after crop emergence (WAE)), barnyardgrass produced c. 35 500 and 16 500 seeds/plant in cotton, and c. 39 000 and 2900 seeds/plant in rice, in 2008 and 2009, respectively. Seed production was observed when seedlings emerged up to 5 WAE (2008) or 7 WAE (2009) in cotton and up to 5 WAE (2008, 2009) in rice; corresponding seed production was c. 2500 and 1500 seeds/plant in cotton, and c. 14 700 and 110 seeds/plant in rice, in 2008 and 2009, respectively. The results suggest that cultural approaches that delay the emergence of barnyardgrass or approaches that make the associated crop more competitive will be useful in integrated management programmes. In the context of herbicide resistance management, it may be valuable to prevent seed return to the seedbank, irrespective of cohorts. The findings are vital for parameterizing herbicide resistance simulation models for barnyardgrass

    Virion Positions and Relationships of Lactococcal Temperate Bacteriophage TP901-1 Proteins

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    AbstractThe major proteins of phage TP901-1 virion were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and structural relations were determined using specific antibodies, obtained by affinity purification from a polyclonal serum. A 23-kDa protein was identified as the major tail protein, and a 31-kDa molecule as the major head protein, respectively. Labeling experiments with antibodies against two proteins, with molecular masses of 20 and 19 kDa, indicated that they were baseplate-related components. A 72-kDa protein was found to be part of a neck passage structure, which includes a collar. Evidence for the presence of attached whiskers was also obtained. T7 RNA polymerase-mediated expression of the two major proteins confirmed the gene location of the previously sequenced region of the phage genome. The relation to other lactococcal phages was determined by DNA hybridization and antibody probing, showing that despite low DNA similarity, TP901-1 NPS epitopes were detected in both related and unrelated small isometric-headed phages

    Persistent Reversal of Enhanced Amphetamine Intake by Transient CaMKII Inhibition

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    Amphetamine exposure transiently increases CaMKIIα expression in the nucleus accumbens (NAcc) shell and this persistently increases local GluA1 S831 phosphorylation and enhances behavioral responding to the drug. Here we assessed whether transiently interfering with CaMKII signaling using a dominant-negative CaMKIIα mutant delivered to the NAcc shell with herpes simplex viral (HSV) vectors could reverse these long-lasting biochemical and behavioral effects observed following exposure to amphetamine. As expected, transient expression of CaMKIIα K42M in the NAcc shell produced a corresponding transient increase in CaMKIIα and decrease in pCaMKIIα (T286) protein levels in this site. Remarkably, this transient inhibition of CaMKII activity produced a long-lasting reversal of the increased GluA1 S831 phosphorylation levels in NAcc shell and persistently blocked the enhanced locomotor response to and self-administration of amphetamine normally observed in rats previously exposed to the drug. Together, these results indicate that even transient interference with CaMKII signaling may confer long-lasting benefits in drug sensitized individuals and point to CaMKII and its downstream pathways as attractive therapeutic targets for the treatment of stimulant addiction

    "Because at school, you can become somebody" - the perceived health and economic returns on secondary schooling in rural Burkina Faso

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    Background The perceived returns on schooling are critical in schooling decision-making but are not well understood. This study examines the perceived returns on secondary schooling in Burkina Faso, where secondary school completion is among the lowest globally (<10%). Methods We conducted a two-staged qualitative study using semi-structured interviews (N = 49). In the first stage, we sampled students, dropouts, parents and teachers from a random sample of five schools (n = 39). In the second stage, we interviewed key informants knowledgeable of the school context using snowball sampling (n = 10). Systematic analysis was based on a grounded theory approach with a reading of transcripts, followed by coding of the narratives in NVivo 12. Results Respondents nearly universally perceived health benefits to schooling. In particular, key health benefits included improved sexual and reproductive health outcomes, hygiene knowledge and practices, as well as better interactions with the formal health system. Common economic returns on schooling included improved employment opportunities and the provision of support to family members, in addition to generally attaining success and recognition. Indirect and long-term health returns, however, were infrequently mentioned by respondents. Conclusions While respondents reported nearly universally short-term health benefits to schooling, responses with regard to economic as well as indirect and long-term health benefits were more ambiguous. Future intervention studies on the perceived returns on formal education are needed to inform policy and reach education and health targets in the region
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