Thermotherapy as effective technology health in preschool education insti

В статье актуализируются вопросы сохранения и укрепления здоровья ребёнка дошкольного возраста. Раскрывается важность и значимость использования в работе с дошкольниками физкультурно-оздоровительной технологии – термотерапияArticle updated the preservation and strengthening of health of preschool children. Demonstrates the importance and significance of use in working with young children physical education and wellness technology – thermotherap

The Issue of Risks of Smart Technologies Proliferation

Technology is developing at a rapid pace and permeates the entire infrastructure of human life, like the blood vessels of the body. Internet technology has brought humanity closer, and information has never been so accessible thanks to the power of the Internet. The impact and development of new technologies of the 20th century is not easy to assess, because they are still largely part of the ongoing process, which may be just beginning. Evaluation of the risks and disadvantages of smart technologies is a prerequisite for the successful implementation of smart technologies in the process of human life.Технологии развиваются стремительными темпами и пронизывают всю инфраструктуру жизнедеятельности человека, подобно кровеносным сосудам организма. Интернет технологии сблизили человечество, а информация никогда не была так доступна благодаря мощи интернета. Влияние и развитие новых технологий 20-го века нелегко оценить, поскольку они до сих пор в значительной степени являются частью продолжающегося процесса. Оценка рисков и недостатков применения смарт технологий является непременным условием успешного внедрения смарт технологий в процесс человеческой жизнедеятельности

Genotypes and Subtypes of Hepatitis B Virus Isolates in the Territory of Siberia

Identified are the occurrence, serotypic and genotypic variations of Hepatitis B virus isolates (HBV) among the Novosibirsk region inhabitants (n=2000), native population of the Alarsk District of the Irkutsk Region (n=487) and Shuryshkarsk Township of the Yamalo-Nenets Autonomous District (n=657). Occurrence rate of hepatitis В surface antigen (HBsAg) among different groups of the Novosibirsk Region population varied within the limits of 3,6-35,0 %. It was 8,2 % in Alarsk District, and 3,2 % in Shuryshkarsk Township. HBV isolates of D genotype (92-97 %) prevail among the population of Siberia; few are the cases of A (1,7 %) and C (1,2-8 %) genotypes. The identified varying occurrence of HBV sub-genotypes and HBsAg subtypes in two aboriginal groups of Siberia (D3 sub-genotype and ayw2 subtype - in the Alarsk District, D2 and ayw3 - in Shuryshkarsk Township) suggests the existence of, at least, two isolated HBV virus populations, circulating among different groups of Siberia native population

WATER CONDITION IN CELLS OF CHLORELLA

The water condition in cages of the paste of chlorella was investigated by the method of thermogravimetric analysis. With increasing heating rate endothermic effect corresponding to the dehydration process is shifted towards higher temperatures. Temperature intervals of chlorella dehydration are defined at rate of heating 2 К/min - 308-368 K, 5 К/min - 323-403 K, and 10 К/min - 348-403 K. Quantitative characteristics of kinetic unequal water in chlorella have been received for each step (∆, ∆Т, a mass fraction (w), energy of activation (Еа)). This process is similar to the process of the dehydration in ion exchange membranes. The derived kinetic characteristics give the possibility to define an optimum temperature interval and rate of drying microalgae for the purpose of increase of periods of storage in the form of paste or a solid substance for the further use as the bioadditive. In addition the presence of three types of water chlorella in a cell set according to NMR with pulsed magnetic field gradient. Since free water is involved in biochemical, chemical and microbiological processes, it is desirable to remove during drying of the preparation. The resulting temperature range of 323-343 K (step 2) at a heating rate of 2 K / min corresponds to a temperature range of drying the chlorella in a production environment. It should be noted that the highest number of algae in a tightly-water (the last stage). Apparently, this is determined by a unique cell structure. Temperature ranges dehydration process are not clear and vary depending on the heating rate, which is fully in line with previous studies of thermal analysis for grains, vegetables and bakery products

GENETIC DIVERSITY AND POSSIBLE ORIGINS OF THE HEPATITIS B VIRUS IN SIBERIAN NATIVES

A total of 381 hepatitis B virus (HBV) DNA sequences collected from nine groups of Siberian native populations were phylogenetically analyzed along with 179 HBV strains sampled in different urban populations of former western USSR republics and 50 strains from Central Asian republics and Mongolia. Different HBV subgenotypes predominated in various native Siberian populations. Subgenotype D1 was dominant in Altaian Kazakhs (100%), Tuvans (100%), and Teleuts (100%) of southern Siberia as well as in Dolgans and Nganasans (69%), who inhabit the polar Taimyr Peninsula. D2 was the most prevalent subgenotype in the combined group of Nenets, Komi, and Khants of the northern Yamalo-Nenets Autonomous Region (71%) and in Yakuts (36%) from northeastern Siberia. D3 was the main subgenotype in South Altaians (76%) and Buryats (40%) of southeastern Siberia, and in Chukchi (51%) of the Russian Far East. Subgenotype C2 was found in Taimyr (19%) and Chukchi (27%), while subgenotype A2 was common in Yakuts (33%). In contrast, D2 was dominant (56%) in urban populations of the former western USSR, and D1 (62%) in Central Asian republics and Mongolia. Statistical analysis demonstrated that the studied groups are epidemiologically isolated from each other and might have contracted HBV from different sources during the settlement of Siberia

Применение GLAD-ПЦР-анализа для изучения метилирования ДНК в регуляторных областях генов-онкосупрессоров при раке молочной железы

Hypermethylation of the RcgY sites is shown for many cancer diseases. such aberrant methylation, suppressing the gene activity, occurs at early stages of carcinogenesis. Recently, using glad-pcR assay, we have detected aberrantly methylated RcgY sites, which can be considered to be epigenetic markers of colorectal, lung, and gastric cancers. in breast cancer, methylation of the regulatory regions of ALX4, BMP2, CCND2, CDH13, CDX1, FOXA1, GALR1, GATA5, GREM1, HIC1, HMX2, HS3ST2, HOXC10, ICAM5, LAMA1, RARB, RASSF1A, RUNX3, RXRG, RYR2, SFRP2, SOX17, TERT, and ZNF613 tumor-suppressor genes is reported. in the present work, we determined aberrantly methylated RcgY sites in the regulatory regions of these genes in dNa preparations from breast cancer tissues. the study of dNa samples from 30 tumor and 22 normal mammary tissue samples demonstrates a high diagnostic potential of selected R(5mc)gY sites in regulatory regions of CCND2, BMP2, GALR1, SOX17, HMX2, and HS3ST2 genes with total index of sensitivity and specificity for R(5mc)gY detection in tumor dNa 90.0 % and 100.0 %, respectively.Аберрантное метилирование ДНК на начальных стадиях канцерогенеза приводит к инактивации ряда генов и показано для многих онкологических заболеваний. Ранее с помощью метода glad-ПЦР анализа нами были выявлены аберрантно метилированные сайты R(5mc)gY, которые могут рассматриваться как эпигенетические маркеры при диагностике колоректального рака, рака легкого и рака желудка. При раке молочной железы, по литературным данным, наблюдается метилирование регуляторных участков генов ALX4, BMP2, CCND2, CDH13, CDX1, FOXA1, GALR1, GATA5, GREM1, HIC1, HMX2, HS3ST2, HOXC10, ICAM5, LAMA1, RARB, RASSF1A, RUNX3, RXRG, RYR2, SFRP2, SOX17, TERT и ZNF613. В настоящей работе было проведено определение аберрантно метилированных сайтов RcgY в регуляторных участках данных генов в препаратах ДНК из тканей рака молочной железы. Анализ образцов ДНК из операционного материала опухолей (n=30) и морфологически неизмененных тканей молочной железы (n=22) показал высокую диагностическую эффективность панели маркеров, включающей сайты R(5mc)gY в составе генов CCND2, BMP2, GALR1, SOX17, HMX2 и HS3ST2: суммарные показатели чувствительности и специфичности составляют 90,0 и 100,0 % соответственно