Self-tuning high-voltage and high-frequency sinusoidal power supply for dielectric barrier discharge plasma generation

In this paper a high-voltage sinusoidal power supply controlled by Arduino DUE micro-controller is described. This generator can feed a dielectric barrier discharge (DBD) load with sinusoidal voltages up to 20 kV peak and frequencies in the range 10\u201360 kHz, with a maximum output power of 200 W. Output voltage can be produced either in a continuous mode, or with on/off modulation cycles, according to treatment/application requirements. This power source is equipped with on-board diagnostics used to measure the output voltage and the charge delivered to the load. With a sample frequency of 500 kHz, Arduino DUE allows to evaluate both the high voltage and the average power feeding the discharge without the use of an expensive external measurement setup. Lissajous techniques are utilized to calculate discharge average power in a quasi-real-time manner. When a load is connected to high-voltage terminals, a self-tuning procedure is carried out to obtain the best working frequency. This parameter allows to minimize power-electronic component stress and to maximize generator efficiency

Networks from gene expression time series: characterization of correlation patterns

This paper describes characteristic features of networks reconstructed from gene expression time series data. Several null models are considered in order to discriminate between informations embedded in the network that are related to real data, and features that are due to the method used for network reconstruction (time correlation).Comment: 10 pages, 3 BMP figures, 1 Table. To appear in Int. J. Bif. Chaos, July 2007, Volume 17, Issue

Inactivation of eimeria Oocysts in aqueous solution by a dielectric barrier discharge plasma in contact with liquid

This study presents a novel technique to inactivate coccidian oocysts in an aqueous solution. The technique consists of treating the contaminated liquid by using an atmospher- ic-pressure air dielectric barrier discharge (DBD) plasma in contact with it. Many experiments in several operating conditions were performed. The discharge was supplied by sinusoidal and nanosecond-pulsed voltages with a constant average power of ~7 W in both cases. Biological effects due to the plasma were investigated by performing tests with increasing treatment time. A sudden ~40% drop in the number of survived oocysts was reached in 4 min and a two-fold reduction was detected after 12 min of exposure. No significant differences in the biocidal efficacy were detected between the AC-driven and the nanosecond-pulsed discharge. Chi-squared statistical analysis on the treated samples showed significant statistical difference (with a statistical significance P value parameter less than 0.01) and nonrandomness warranty of the results, opening interesting scenarios for fimire developments

Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability

Background: Histone modification H4K20me3 and its methyltransferase SUV420H2 have been implicated in suppression of tumorigenesis. The underlying mechanism is unclear, although H4K20me3 abundance increases during cellular senescence, a stable proliferation arrest and tumor suppressor process, triggered by diverse molecular cues, including activated oncogenes. Here, we investigate the function of H4K20me3 in senescence and tumor suppression. Results: Using immunofluorescence and ChIP-seq we determine the distribution of H4K20me3 in proliferating and senescent human cells. Altered H4K20me3 in senescence is coupled to H4K16ac and DNA methylation changes in senescence. In senescent cells, H4K20me3 is especially enriched at DNA sequences contained within specialized domains of senescence-associated heterochromatin foci (SAHF), as well as specific families of non-genic and genic repeats. Altered H4K20me3 does not correlate strongly with changes in gene expression between proliferating and senescent cells; however, in senescent cells, but not proliferating cells, H4K20me3 enrichment at gene bodies correlates inversely with gene expression, reflecting de novo accumulation of H4K20me3 at repressed genes in senescent cells, including at genes also repressed in proliferating cells. Although elevated SUV420H2 upregulates H4K20me3, this does not accelerate senescence of primary human cells. However, elevated SUV420H2/H4K20me3 reinforces oncogene-induced senescence-associated proliferation arrest and slows tumorigenesis in vivo. Conclusions: These results corroborate a role for chromatin in underpinning the senescence phenotype but do not support a major role for H4K20me3 in initiation of senescence. Rather, we speculate that H4K20me3 plays a role in heterochromatinization and stabilization of the epigenome and genome of pre-malignant, oncogene-expressing senescent cells, thereby suppressing epigenetic and genetic instability and contributing to long-term senescence-mediated tumor suppression

Effect of nanofillers on surface charge mobility and alternative technique for electrostatic field measurement

This paper has the main purpose to assess the influence of nanofillers on the mobility of electrostatic surface charge buildup. Electro Hydro Dynamic actuators were produced and tested at a fixed voltage amplitude and frequency, using different thermoplastic dielectrics, some of which belonging to the family of nano-filled materials. The investigation focuses on the mobility of superficial electrostatic buildup, also experimenting the validity of an alternative high voltage potential measurement technique based on monitoring of multiple embedded electrodes. Results show that nanofillers have a profound effect on electrostatic charge buildup, strongly reducing their mobility

High-voltage high-frequency arbitrary waveform multilevel generator for dielectric barrier discharge2014 International Symposium on Power Electronics, Electrical Drives, Automation and Motion

A high-voltage high-frequency arbitrary waveform voltage generator able to supply a wide range of plasma reactors is proposed in this paper. The generator structure is based on the cascaded H-bridge multilevel topology, including 24 H-bridge basic modules with the capability of 49 output voltage levels. The individual H-bridge dc supply (600V) is provided by a flyback converter fed by a low-voltage (12V) dc battery. In this way, the input isolation of the \ub115kV maximum output voltage is realized in a simple and effective manner, and a portable device is obtained. The generator has been tested by supplying a DBD plasma actuator with different voltage waveforms, up to 20 kHz, pointing out a wide and interesting set of results in terms of output plasma currents