T cell immune response predicts survival in severely ill COVID-19 patients requiring venovenous extracorporeal membrane oxygenation support

IntroductionThere is a critical gap in understanding which SARS-CoV-2 patients would benefit most from venovenous extracorporeal membrane oxygenation (VV-ECMO) support. The potential role of a dysregulated immune response is still unclear in this patient population.ObjectivesTo assess the potential predictive value of SARS-CoV-2 specific cellular and humoral immune responses for survival in critically ill COVID-19 patients requiring VV-ECMO.MethodsWe conducted a prospective single-center observational study of unvaccinated patients requiring VV-ECMO support treated at the intensive care unit of Semmelweis University Heart and Vascular Center between March and December 2021. Peripheral blood samples were collected to measure the humoral and cellular immune statuses of the patients at the VV-ECMO cannulation. Patients were followed until hospital discharge.ResultsOverall, 35 COVID-19 patients (63% men, median age 37 years) on VV-ECMO support were included in our study. The time from COVID-19 verification to ECMO support was a median (IQR) of 10 (7-14) days. Of the patients, 9 (26%) were discharged alive and 26 (74%) died during their hospital stay. Immune tests confirmed ongoing SARS-CoV-2 infection in all the patients, showing an increased humoral immune response. SARS-CoV-2-specific cellular immune response was significantly higher among survivors compared to the deceased patients. A higher probability of survival was observed in patients with markers indicating a higher T cell response detected by both QuantiFeron (QF) and flow cytometry (Flow) assays. (Flow S1 CD8+ ≥ 0.15%, Flow S1 CD4+ ≥ 0.02%, QF CD4 ≥ 0.07, QF whole genome ≥ 0.59). In univariate Cox proportional hazard regression analysis BMI, right ventricular (RV) failure, QF whole genome T cell level, and Flow S1 CD8+ T cell level were associated with mortality, and we found that an increased T cell response showed a significant negative association with mortality, independent of BMI and RV failure.ConclusionEvaluation of SARS-CoV-2 specific T cell response before the cannulation can aid the risk stratification and evaluation of seriously ill COVID-19 patients undergoing VV-ECMO support by predicting survival, potentially changing our clinical practice in the future

Exercise epidemiology on mortality and morbidity with an emphasis on the effects of physical fitness

Metabolic syndrome, a cluster of metabolic and cardiovascular disorders has risen worldwide. The issue gets increasing attention because its progressive form lead to type 2 diabetes mellitus, cardiovascular diseases and premature mortality. Intervention tools are required to prevent them. Physical fitness is recognized to be a first step intervention tool. There are two purposes of this review: to give a summary of the metabolic syndrome and physical fitness; to examine two relationships, namely, the relationship between metabolic syndrome and physical fitness and the relationship between all-cause mortality and handgrip strength

Exercise epidemiology on mortality and morbidity with an emphasis on the effects of physical fitness

Metabolic syndrome, a cluster of metabolic and cardiovascular disorders has risen worldwide. The issue gets increasing attention because its progressive form lead to type 2 diabetes mellitus, cardiovascular diseases and premature mortality. Intervention tools are required to prevent them. Physical fitness is recognized to be a first step intervention tool. There are two purposes of this review: to give a summary of the metabolic syndrome and physical fitness; to examine two relationships, namely, the relationship between metabolic syndrome and physical fitness and the relationship between all-cause mortality and handgrip strength