MicroRNA Regulation of CD16 in Human Natural Killer Cells

Natural killer (NK) cells are lymphocytes capable of killing virus-infected and tumor cells while leaving normal tissue unharmed. CD16 is an Fc receptor that allows NK cells to perform antibody-dependent cell-mediated cytotoxicity (ADCC) against tumor cells. We hypothesize that miRNAs (miRs) have a regulatory role for CD16 expression in NK cells. We compiled a list of 69 miRs predicted in silico to target CD16 mRNA. This list was cross-referenced with a gene expression data set of 400 miRs measured in peripheral blood (PB) from 4 healthy donors. Of the 69 miRs candidates, 3 were found to be downregulated in the CD16+ NK cell populations compared to CD16- populations. RT-qPCR showed that CD16- human NK cells in PB had approximately 25 fold higher mir-218 expression than CD16+ populations (p=0.008, n=4). RT-qPCR was also used to measure miR-218 expression during NK cell maturation stages 3, 4a, 4b, and 5 NK cells from 5 donors. The higher expression of miR-218 in stage 4a and 4b developing NK cells correlated to their low expression of CD16. Using 293T cells infected with miR-218 pCDH luciferase construct, we were able to demonstrate a 35% reduction (p=0.12, n=3) in luciferase activity with overexpression of miR-218. Thus, we have preliminary evidence that miR-218 is an intrinsic negative regulator of CD16 expression in NK cells. Ongoing work will determine the effect of overexpression and knockdown of miR-218 on CD16 expression, ADCC killing, and cytokine production in a CD16+ cell line and primary NK cells.A one-year embargo was granted for this item.Academic Major: Human Nutritio

Technology of the Oppressed

How Brazilian favela residents engage with and appropriate technologies, both to fight the oppression in their lives and to represent themselves in the world. Brazilian favelas are impoverished settlements usually located on hillsides or the outskirts of a city. In Technology of the Oppressed, David Nemer draws on extensive ethnographic fieldwork to provide a rich account of how favela residents engage with technology in community technology centers and in their everyday lives. Their stories reveal the structural violence of the information age. But they also show how those oppressed by technology don't just reject it, but consciously resist and appropriate it, and how their experiences with digital technologies enable them to navigate both digital and nondigital sources of oppression—and even, at times, to flourish. Nemer uses a decolonial and intersectional framework called Mundane Technology as an analytical tool to understand how digital technologies can simultaneously be sites of oppression and tools in the fight for freedom. Building on the work of the Brazilian educator and philosopher Paulo Freire, he shows how the favela residents appropriate everyday technologies—technological artifacts (cell phones, Facebook), operations (repair), and spaces (Telecenters and Lan Houses)—and use them to alleviate the oppression in their everyday lives. He also addresses the relationship of misinformation to radicalization and the rise of the new far right. Contrary to the simplistic techno-optimistic belief that technology will save the poor, even with access to technology these marginalized people face numerous sources of oppression, including technological biases, racism, classism, sexism, and censorship. Yet the spirit, love, community, resilience, and resistance of favela residents make possible their pursuit of freedom

Detection of C-type natriuretic peptide (CNP) transcript in the rat heart and immune organs

Previous studies suggested the expression of mRNA, coding for CNP, exclusively in the central nervous system. In the present study, using the polymerase chain reaction (PCR) technique instead of the less sensitive Northern blot hybridization, CNP-specific sequences have also been detected in rat atria and ventricles of the heart as well as in organs of the immune system (thymus, spleen and lymph nodes). Parallel PCR-assays documented ANP-mRNA in these tissues. To verify specificity of the PCR-products, Southern blots have been hybridized with a third internal oligonucleotide and amplification products have been sequenced. The relative level of CNP-mRNA in these tissues was estimated to be in the range of 1-9% of total brain CNP transcripts. The results suggest that the peptide may have a peripheral as well as a central site of action. In light of its pronounced effect on cell proliferation, particular interest should focus on a possible role of CNP in the immune system

Transcription of Brain Natriuretic Peptide and Atria1 Natriuretic Peptide Genes in Human Tissues.

We have compared the expression of atria1 natriuretic peptide (ANP) and brain natriuretic peptide (BNP) genes in various human tissues using a quantitative polymerase chain reaction technique. Tissues of three human subjects, obtained at autopsy, were analyzed. BNP transcripts could be detected in the central nervous system, lung, thyroid, adrenal, kidney, spleen, small intestine, ovary, uterus, and striated muscle. ANP transcripts could also be demonstrated in various human extracardiac tissues including several endocrine organs. In all periphera1 tissues, the level of both natriuretic peptide transcripts was approximately l-2 orders of magnitude lower than in cardiac ventricular tissues. This distribution is in marked contrast to the much lower level of ANP and BNP transcripts present in extracardiac rat tissues (generally less than l/1000 of ventricles). These data suggest differential expression of the two natriuretic peptide genes in cardiac and extracardiac tissues in man. Furthermore, the presence of local synthesis of ANP and BNP in various peripheral organs su gests paracrine and/or autocrine function of these natriuretic peptides

Transcription of brain natriuretic peptide and atrial natriuretic peptide genes in human tissues

We have compared the expression of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) genes in various human tissues using a quantitative polymerase chain reaction technique. Tissues of three human subjects, obtained at autopsy, were analyzed. BNP transcripts could be detected in the central nervous system, lung, thyroid, adrenal, kidney, spleen, small intestine, ovary, uterus, and striated muscle. ANP transcripts could also be demonstrated in various human extracardiac tissues including several endocrine organs. In all peripheral tissues, the level of both natriuretic peptide transcripts was approximately 1-2 orders of magnitude lower than in cardiac ventricular tissues. This distribution is in marked contrast to the much lower level of ANP and BNP transcripts present in extracardiac rat tissues (generally less than 1/1000 of ventricles). These data suggest differential expression of the two natriuretic peptide genes in cardiac and extracardiac tissues in man. Furthermore, the presence of local synthesis of ANP and BNP in various peripheral organs suggests paracrine and/or autocrine function of these natriuretic peptides