EFFECTS OF HERBICIDES AND GRAZING ON FLORISTIC QUALITY OF NATIVE TALLGRASS PASTURES IN EASTERN SOUTH DAKOTA AND SOUTHWESTERN MINNESOTA

Historic herbicide use and grazing have influenced natural diversity and quality of native pasturelands in the Great Plains. Floristic quality assessments are useful to assist agencies in prioritizing conservation practices to enhance native grasslands. The objective of this study was to determine the effects of past land-use practices on the floristic quality of remnant native pastures in eastern South Dakota and southwestern Minnesota. Floristic quality assessments were conducted on 30 native pastures and categorized by past management practices (herbicide application and grazing intensity). Mean coefficient of conservatism (C) and floristic quality index (FQI) were calculated for each site~Results showed that increased herbicide use and grazing intensity resulted in a lower species richness, forb C ,and FQI. However, grass and grasslike plants were minimally affected. Pastures that were infrequently sprayed with herbicides and lightly grazed consistently had the highest species richness, C ,and FQI. Pastures with no grazing produced similar values to those with moderate grazing. Pastures managed as preserves or wildlife habitat areas had higher FQI than those managed for livestock grazing. The implications of this study should further help ecologists and managers understand the positive and negative effects of grazing practices and herbicide application on tallgrass prairie remnants

EFFECTS OF HERBICIDES AND GRAZING ON FLORISTIC QUALITY OF NATIVE TALLGRASS PASTURES IN EASTERN SOUTH DAKOTA AND SOUTHWESTERN MINNESOTA

Historic herbicide use and grazing have influenced natural diversity and quality of native pasturelands in the Great Plains. Floristic quality assessments are useful to assist agencies in prioritizing conservation practices to enhance native grasslands. The objective of this study was to determine the effects of past land-use practices on the floristic quality of remnant native pastures in eastern South Dakota and southwestern Minnesota. Floristic quality assessments were conducted on 30 native pastures and categorized by past management practices (herbicide application and grazing intensity). Mean coefficient of conservatism (C) and floristic quality index (FQI) were calculated for each site~Results showed that increased herbicide use and grazing intensity resulted in a lower species richness, forb C ,and FQI. However, grass and grasslike plants were minimally affected. Pastures that were infrequently sprayed with herbicides and lightly grazed consistently had the highest species richness, C ,and FQI. Pastures with no grazing produced similar values to those with moderate grazing. Pastures managed as preserves or wildlife habitat areas had higher FQI than those managed for livestock grazing. The implications of this study should further help ecologists and managers understand the positive and negative effects of grazing practices and herbicide application on tallgrass prairie remnants

Polarization of macrophages toward M2 phenotype is favored by reduction in iPLA2β (group VIA phospholipase A2)*

Macrophages are important in innate and adaptive immunity. Macrophage participation in inflammation or tissue repair is directed by various extracellular signals and mediated by multiple intracellular pathways. Activation of group VIA phospholipase A2 (iPLA2β) causes accumulation of arachidonic acid, lysophospholipids, and eicosanoids that can promote inflammation and pathologic states. We examined the role of iPLA2β in peritoneal macrophage immune function by comparing wild type (WT) and iPLA2β−/− mouse macrophages. Compared with WT, iPLA2β−/− macrophages exhibited reduced proinflammatory M1 markers when classically activated. In contrast, anti-inflammatory M2 markers were elevated under naïve conditions and induced to higher levels by alternative activation in iPLA2β−/− macrophages compared with WT. Induction of eicosanoid (12-lipoxygenase (12-LO) and cyclooxygenase 2 (COX2))- and reactive oxygen species (NADPH oxidase 4 (NOX4))-generating enzymes by classical activation pathways was also blunted in iPLA2β−/− macrophages compared with WT. The effects of inhibitors of iPLA2β, COX2, or 12-LO to reduce M1 polarization were greater than those to enhance M2 polarization. Certain lipids (lysophosphatidylcholine, lysophosphatidic acid, and prostaglandin E2) recapitulated M1 phenotype in iPLA2β−/− macrophages, but none tested promoted M2 phenotype. These findings suggest that (a) lipids generated by iPLA2β and subsequently oxidized by cyclooxygenase and 12-LO favor macrophage inflammatory M1 polarization, and (b) the absence of iPLA2β promotes macrophage M2 polarization. Reducing macrophage iPLA2β activity and thereby attenuating macrophage M1 polarization might cause a shift from an inflammatory to a recovery/repair milieu

The Case of Ty Jackson: An Interactive Module on LGBT Health Employing Introspective Techniques and Video-Based Case Discussion

Introduction: The Institute of Medicine's 2011 report on lesbian, gay, bisexual, and transgender (LGBT) health and the legalization of same-sex marriage are just two of the numerous milestones that have hastened medical schools' efforts to prepare trainees to address the needs of LGBT community members. Early awareness of sexual diversity through self- and peer introspection and video-based education can help trainees build a foundation towards providing affirming care to LGBT patients. Methods: The Kern model was used to develop, implement, and evaluate an interactive multimodal workshop to provide first-year medical students with a formative introduction to LGBT health. Learning objectives focused on comprehending the spectrum of human sexuality, health issues for LGBT patients, and better practices for promoting affirming care. The module consisted of a PowerPoint presentation, sexuality survey, videos of provider-patient encounters, and community-based resources. Results: The workshop was implemented among 178 first-year medical students in September 2018, with 93% completing the pre-/postworkshop evaluations. Comparison of evaluations showed an increase in confidence in addressing each of the three learning objectives. Over 85% rated the PowerPoint and videos as very good or excellent. Discussion: This workshop was effective in helping first-year medical students appreciate the spectrum of sexual diversity, health issues facing LGBT individuals, and better practices to promote affirming care. The real-time sexuality survey helped trainees appreciate sexual diversity through self-reflection and near-peer sharing. The videos and accompanying discussion provided real-life encounters, along with common pitfalls in and pearls for communicating with LGBT patients

Torsional Directed Walks, Entropic Elasticity, and DNA Twist Stiffness

DNA and other biopolymers differ from classical polymers due to their torsional stiffness. This property changes the statistical character of their conformations under tension from a classical random walk to a problem we call the `torsional directed walk'. Motivated by a recent experiment on single lambda-DNA molecules [Strick et al., Science 271 (1996) 1835], we formulate the torsional directed walk problem and solve it analytically in the appropriate force regime. Our technique affords a direct physical determination of the microscopic twist stiffness C and twist-stretch coupling D relevant for DNA functionality. The theory quantitatively fits existing experimental data for relative extension as a function of overtwist over a wide range of applied force; fitting to the experimental data yields the numerical values C=120nm and D=50nm. Future experiments will refine these values. We also predict that the phenomenon of reduction of effective twist stiffness by bend fluctuations should be testable in future single-molecule experiments, and we give its analytic form.Comment: Plain TeX, harvmac, epsf; postscript available at http://dept.physics.upenn.edu/~nelson/index.shtm

Comparing placentas from normal and abnormal pregnancies

This report describes work carried out at a Mathematics-in-Medicine Study Group. It is believed that placenta shape villous network characteristics are strongly linked to the placenta’s efficiency, and hence to pregnancy outcome. We were asked to consider mathematical ways to describe the shape and other characteristics of a placenta, as well as forming mathematical models for placenta development. In this report we propose a number of possible measure of placental shape, form, and efficiency, which can be computed from images already obtained. We also consider various models for the early development of placentas and the growth of the villous tree

Light Induced Melting of Colloidal Crystals in Two Dimensions

We demonstrate that particles confined to two dimensions (2d) and subjected to a one-dimensional (1d) periodic potential exhibit a rich phase diagram, with both ``locked floating solids'' and smectic phases. The resulting phases and phase transitions are studied as a function of temperature and potential strength. We find reentrant melting as a function of the potential strength. Our results lead to universal predictions consistent with recent experiments on 2d colloids in the presence of a laser-induced 1d periodic potential.Comment: 4 pages, 3 figures, also available at http://cmtw.harvard.edu/~fre

Linear density perturbations in multifield coupled quintessence

18 pages, 8 figures, version to be submitted to PRD, refs. added19 pages, 9 figures, version accepted for publication by PRD, refs. added, extra Consistency Test section added19 pages, 9 figures, version accepted for publication by PRD, refs. added, extra Consistency Test section adde

Vortices and 2D bosons: A Path-Integral Monte Carlo Study

The vortex system in a high-T_c superconductor has been studied numerically using the mapping to 2D bosons and the path-integral Monte Carlo method. We find a single first-order transition from an Abrikosov lattice to an entangled vortex liquid. The transition is characterized by an entropy jump dS = 0.4 k_B per vortex and layer (parameters for YBCO) and a Lindemann number c_L = 0.25. The increase in density at melting is given by d\rho = 6.0*10^{-4} / \lambda(T)^2. The vortex liquid corresponds to a bosonic superfluid, with \rho_s = \rho even in the limit \lambda -> \infty.Comment: 9 pages, RevTeX, 4 PostScript figures. The entropy jump at the transition has been recomputed and is now in agreement with experiments on YBCO. Some minor modifications were made in the tex