Detection and quantification of viable airborne bacteria and fungi using solid-phase cytometry

This protocol describes the use of solid-phase cytometry for the enumeration of airborne bacteria and fungi. In contrast with conventional methods, accurate results can be obtained in real time, especially for air samples with low numbers of microorganisms. Air samples are collected by impaction on a water-soluble polymer that is subsequently dissolved. Part of the sample can be filtered over two membrane filters with different pore sizes. One filter is used to obtain a total count of all viable microorganisms, and a second filter is used to determine the number of airborne fungi. Microorganisms present on the filter are labeled with a viability substrate and subsequently detected and quantified using a solid-phase cytometer. The detected spots are microscopically validated using an epifluorescence microscope to discriminate between bacteria, fungi and fluorescent particles. The whole procedure takes 5 h to complete and results in the accurate quantification of airborne bacteria and fungi for samples with a low or high microbial load

DYAMAND: dynamic, adaptive management of networks and devices

Consumer devices increasingly are "smart" and hence offer services that can interwork with and/or be controlled by others. However, the full exploitation of the inherent opportunities this offers, is hurdled by a number of potential limitations. First of all, the interface towards the device might be vendor and even device specific, implying that extra effort is needed to support a specific device. Standardization efforts try to avoid this problem, but within a certain standard ecosystem the level of interoperability can vary (i.e. devices carrying the same standard logo are not necessarily interoperable). Secondly, different application domains (e.g. multimedia vs. energy management) today have their own standards, thus limiting trans-sector innovation because of the additional effort required to integrate devices from traditionally different domains into novel applications. In this paper, we discuss the basic components of current so-called service discovery protocols (SDPs) and present our DYAMAND (DYnamic, Adaptive MAnagement of Networks and Devices) framework. We position this framework as a middleware layer between applications and discoverable/controllable devices, and hence aim to provide the necessary tool to overcome the (intra- and inter-domain) interoperability gaps previously sketched. Thus, we believe it can act as a catalyst enabling trans-sector innovation

HUBUNGAN STATUS GIZI DENGAN WAKTU MENSTRUASI PERTAMA (MENARCHE) PADA SISWI SMP TEUKU NYAK ARIF FATIH BILINGUAL SCHOOL TAHUN 2011

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MORA: an Energy-Aware Slack Reclamation Scheme for Scheduling Sporadic Real-Time Tasks upon Multiprocessor Platforms

In this paper, we address the global and preemptive energy-aware scheduling problem of sporadic constrained-deadline tasks on DVFS-identical multiprocessor platforms. We propose an online slack reclamation scheme which profits from the discrepancy between the worst- and actual-case execution time of the tasks by slowing down the speed of the processors in order to save energy. Our algorithm called MORA takes into account the application-specific consumption profile of the tasks. We demonstrate that MORA does not jeopardize the system schedulability and we show by performing simulations that it can save up to 32% of energy (in average) compared to execution without using any energy-aware algorithm.Comment: 11 page

Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite

Background: Burkholderia cepacia complex bacteria are opportunistic pathogens, which can cause severe respiratory tract infections in patients with cystic fibrosis (CF). As treatment of infected CF patients is problematic, multiple preventive measures are taken to reduce the infection risk. Besides a stringent segregation policy to prevent patient-to-patient transmission, clinicians also advise patients to clean and disinfect their respiratory equipment on a regular basis. However, problems regarding the efficacy of several disinfection procedures for the removal and/or killing of B. cepacia complex bacteria have been reported. In order to unravel the molecular mechanisms involved in the resistance of biofilm-grown Burkholderia cenocepacia cells against high concentrations of reactive oxygen species (ROS), the present study focussed on the transcriptional response in sessile B. cenocepacia J2315 cells following exposure to high levels of H2O2 or NaOCl. Results: The exposure to H2O2 and NaOCl resulted in an upregulation of the transcription of 315 (4.4%) and 386 (5.4%) genes, respectively. Transcription of 185 (2.6%) and 331 (4.6%) genes was decreased in response to the respective treatments. Many of the upregulated genes in the NaOCl- and H2O2-treated biofilms are involved in oxidative stress as well as general stress response, emphasizing the importance of the efficient neutralization and scavenging of ROS. In addition, multiple upregulated genes encode proteins that are necessary to repair ROS-induced cellular damage. Unexpectedly, a prolonged treatment with H2O2 also resulted in an increased transcription of multiple phage-related genes. A closer inspection of hybridisation signals obtained with probes targeting intergenic regions led to the identification of a putative 6S RNA. Conclusion: Our results reveal that the transcription of a large fraction of B. cenocepacia J2315 genes is altered upon exposure of sessile cells to ROS. These observations have highlighted that B. cenocepacia may alter several pathways in response to exposure to ROS and they have led to the identification of many genes not previously implicated in the stress response of this pathogen