Characterization of tissue specific expressed proteins and metabolites in Cenchrus polystachion (L.) Schult and their role in apomixis

Agricultural production can be aggrandized by adaptation of trending technologies or processes which focus on increase in seed production. Asexual reproduction in plants or Apomixis is such a process, but its absence in major crop plants has paved an increased research towards understanding the process of apomixis. In present study an attempt was made to understand the role of differentially expressed proteins and metabolite in plant parts like leaf, stigma, immature ovary, seed, anther sac and pollen grains of Cenchrus polystachion. 563, 936, 1188, 770, 721 and 712 proteins and 6118, 6784, 6192, 6615, 5797 and 5791 metabolites were obtained from leaf, stigma, immature ovary, seed, anther sac and pollen respectively on proteome and metabolome analysis. Some of the differentially expressed proteins and metabolites unveiled the important pathways for apomixis in Cenchrus. The top most pathways involved in apomixis are sphingolipid metabolism, glycerophospholipid metabolism, pantothenate and CoA biosynthesis, alpha linolenic acid pathway and brassinosteroid biosynthesis. The detailed analysis of the all the tissues gave an insight of the overexpression of GNDI Inhibitor (Guanosine nucleotide diphosphate dissociation inhibitor) in immature ovary. The molecular docking study further revealed that the GoLOCO motif of GNDI efficiently interacts with G alpha protein which interferes with the binding of G alpha with PLD alpha (Phospholipase D alpha). Thus, the overexpression of G alpha Inhibitor might exert their effect on PLD alpha leading to meiosis inactivation and formation of apomictic seed

Analysis of Physicochemical Properties, Available Nutrients of Soil and their Correlation with Incidence of Telya Disease of Pomegranate at Northern Nasik, Maharashtra

Maharashtra government reported 10,000 crore production loss of Pomegranate every year, due to the incidence of Telya disease. The present study was aimed to analyse the physical, chemical properties, and available micro-macronutrients in the soil of pomegranate orchards infected with Telya disease. Estimation of incidence and severity of disease was done on fifty selected orchards from different villages that were MangiTungi, Daswel, Dasane, Mulher, and Sompur. Results revealed that minimum incidence (58.66%) and severity (59.89%) were recorded in Sompur village whereas maximum incidence (74.40%) and severity (68.70%) were recorded in Daswel and MangiTungi village respectively. The pH (7.5-7.9) and free lime concentration 7.4-9.4%) were exceptionally very high for all test and control samples. Deficiency of essential macronutrients N (<150-250 Kg/ha) and K (<125-200 Kg/ha) was recorded in all test samples along with additional deficiency of Zn micronutrient (<1.0-2.0 ppm). In the case of mock orchards, all the parameters were in accordance with reference values.Statistical analysis of data declared that there was a significant difference among parameters of tested groups (P>0.05) while for control fields there were no significant differences (P<0.05). Further, a positive correlation between macro-micronutrients (Na, Ca, N, P, K, Mn) and incidence of disease was recorded which concludes that an imbalance in nutrients promotes growth of pathogens and increases susceptibility of plants to pathogenic attack. Further, balancing nutrients through fertilisers or foliar spray could be an effective strategy for an integrated pest management system

Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments

Pearl millet [Pennisetum glaucum (L.) R. Br., syn. Cenchrus americanus (L.) Morrone], is a staple food for over 90 million poor farmers in arid and semi-arid regions of sub-Saharan Africa and South Asia. We report the ~1.79 Gb genome sequence of reference genotype Tift 23D2B1-P1-P5, which contains an estimated 38,579 genes. Resequencing analysis of 994 (963 inbreds of the highly cross-pollinated cultigen, and 31 wild accessions) provides insights into population structure, genetic diversity, evolution and domestication history. In addition we demonstrated the use of re-sequence data for establishing marker trait associations, genomic selection and prediction of hybrid performance and defining heterotic pools. The genome wide variations and abiotic stress proteome data are useful resources for pearl millet improvement through deploying modern breeding tools for accelerating genetic gains in pearl millet.publishersversionPeer reviewe

Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte, Sesuvium portulacastrum (L.) L.

In this study, we report phytoremediation of textile dyes using hairy roots derived through Agrobacterium rhizogenes (NCIM 5140) infection of in vitro leaf and stem explants of a halophyte Sesuvium portulacastrum (L.) L. Leaf explants showed higher frequency of hairy root induction (70%) than stem explants (30%), and maximum number of roots (leaf 42.3 ± 2.4 and stem 50.3 ± 1.7). Transformed nature of hairy roots was ascertained by amplifying 970 bp region of T-DNA of Ri plasmid. Hairy roots were screened for phytoremediation of various textile dyes and results showed that HRs were able to degrade Reactive green 19A HE4BD upto 98% within 5 days of incubation. Spectrophotometric analysis showed decrease in dye concentration while HPLC and FTIR analysis confirmed its degradation. Seed germination assay demonstrated non-toxic nature of the extracted metabolites. This is the first report on induction of hairy root culture in Sesuvium portulacastrum and phytoremediation of textile dyes

Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments

International audiencePearl millet [Cenchrus americanus (L.) Morrone] is a staple food for more than 90 million farmers in arid and semi-arid regions of sub-Saharan Africa, India and South Asia. We report the ~1.79 Gb draft whole genome sequence of reference genotype Tift 23D2B1-P1-P5, which contains an estimated 38,579 genes. We highlight the substantial enrichment for wax biosynthesis genes, which may contribute to heat and drought tolerance in this crop. We resequenced and analyzed 994 pearl millet lines, enabling insights into population structure, genetic diversity and domestication. We use these resequencing data to establish marker trait associations for genomic selection, to define heterotic pools, and to predict hybrid performance. We believe that these resources should empower researchers and breeders to improve this important staple crop

Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments

International audiencePearl millet [Cenchrus americanus (L.) Morrone] is a staple food for more than 90 million farmers in arid and semi-arid regions of sub-Saharan Africa, India and South Asia. We report the ~1.79 Gb draft whole genome sequence of reference genotype Tift 23D2B1-P1-P5, which contains an estimated 38,579 genes. We highlight the substantial enrichment for wax biosynthesis genes, which may contribute to heat and drought tolerance in this crop. We resequenced and analyzed 994 pearl millet lines, enabling insights into population structure, genetic diversity and domestication. We use these resequencing data to establish marker trait associations for genomic selection, to define heterotic pools, and to predict hybrid performance. We believe that these resources should empower researchers and breeders to improve this important staple crop

La séquençage du génome du millet perlé fournit une ressource pour améliorer les caractères agronomiques en milieux arides

International audienceAbstract Pearl millet [ Cenchrus americanus (L.) Morrone] is a staple food for more than 90 million farmers in arid and semi-arid regions of sub-Saharan Africa, India and South Asia. We report the ∼1.79 Gb draft whole genome sequence of reference genotype Tift 23D 2 B 1 -P1-P5, which contains an estimated 38,579 genes. We highlight the substantial enrichment for wax biosynthesis genes, which may contribute to heat and drought tolerance in this crop. We resequenced and analyzed 994 pearl millet lines, enabling insights into population structure, genetic diversity and domestication. We use these resequencing data to establish marker trait associations for genomic selection, to define heterotic pools, and to predict hybrid performance. We believe that these resources should empower researchers and breeders to improve this important staple crop.Le millet perlé [Cenchrus americanus (L.) Morrone, syn. Pennisetum glaucum] est un aliment de base pour plus de 90 millions d'agriculteurs dans les régions arides et semi-arides de l'Afrique subsaharienne, de l'Inde et de l'Asie du Sud. Nous rendons compte des résultats du projet de séquençage du génome entier de ~1.79 Gb du génotype de référence Tift 23D2B1-P1-P5 contenant environ 38.579 gènes. Nous soulignons l'enrichissement substantiel en gènes de biosynthèse de la cire, qui peuvent contribuer à la tolérance à la chaleur et à la sécheresse de cette céréale. Nous avons reséquencé et analysé 994 lignées de millet perlé, ce qui nous a permis de mieux appréhender la structure des populations, la diversité génétique et le processus de domestication. Nous utilisons ces données de reséquençage pour établir des associations de marqueurs utiles à la sélection génomique, pour définir des pools hétérotiques et pour prédire la performance des hybrides. Nous pensons que ces ressources devraient permettre aux chercheurs et aux sélectionneurs d'améliorer les performances de cette agriculture vivrière