Use of Macroinvertebrates to Identify Cultivated Wetlands in the Prairie Pothole Region

We evaluated the use of macroinvertebrates as a potential tool to identify dry and intensively farmed temporary and seasonal wetlands in the Prairie Pothole Region. The techniques we designed and evaluated used the dried remains of invertebrates or their egg banks in soils as indicators of wetlands. For both the dried remains of invertebrates and their egg banks, we weighted each taxon according to its affinity for wetlands or uplands. Our study clearly demonstrated that shells, exoskeletons, head capsules, eggs, and other remains of macroinvertebrates can be used to identify wetlands, even when they are dry, intensively farmed, and difficult to identify as wetlands using standard criteria (i.e., hydrology, hydrophytic vegetation, and hydric soils). Although both dried remains and egg banks identified wetlands, the combination was more useful, especially for identifying drained or filled wetlands. We also evaluated the use of coarse taxonomic groupings to stimulate use of the technique by nonspecialists and obtained satisfactory results in most situations

Active Polymers Confer Fast Reorganization Kinetics

Many cytoskeletal biopolymers are "active," consuming energy in large quantities. In this Letter, we identify a fundamental difference between active polymers and passive, equilibrium polymers: for equal mean lengths, active polymers can reorganize faster than equilibrium polymers. We show that equilibrium polymers are intrinsically limited to linear scaling between mean lifetime and mean length, MFPT ~ , by analogy to 1-d Potts models. By contrast, we present a simple active-polymer model that improves upon this scaling, such that MFPT ~ ^{1/2}. Since to be biologically useful, structural biopolymers must typically be many monomers long, yet respond dynamically to the needs of the cell, the difference in reorganization kinetics may help to justify active polymers' greater energy cost. PACS numbers: 87.10.Ed, 87.16.ad, 87.16.L

Linking Ecosystem Processes to Sustainable Wetland Management

The sustainability of ecosystems has become an explicitly stated goal of many natural resource agencies. Examples of sustainable ecosystem management, however, are uncommon because management goals often focus on specific deliverables rather than the processes that sustain ecosystems

Application of a Geomorphic and Temporal Perspective to Wetland Management

The failure of managed wetlands to provide a broad suite of ecosystem services (e.g., carbon storage, wildlife habitat, ground-water recharge, storm-water retention) valuable to society is primarily the result of a lack of consideration of ecosystem processes that maintain productive wetland ecosystems or physical and social forces that restrict a manager’s ability to apply actions that allow those processes to occur. Therefore, we outline a course of action that considers restoration of ecosystem processes in those systems where off-site land use or physical alterations restrict local management. Upon considering a wetland system, or examining a particular management regime, there are several factors that will allow successful restoration of wetland services. An initial step is examination of the political/social factors that have structured the current ecological condition and whether those realities can be addressed. Most successful restorations of wetland ecosystem services involve cooperation among multiple agencies, acquisition of funds from non-traditional sources, seeking of scientific advice on ecosystem processes, and cultivation of good working relationships among biologists, managers, and maintenance staff. Beyond that, in on-site wetland situations, management should examine the existing hydrogeomorphic situation and processes (e.g., climatic variation, tides, riverine flood-pulse events) responsible for maintenance of ecosystem services within a given temporal framework appropriate for that wetland’s hydrologic pattern. We discuss these processes for five major wetland types (depressional, lacustrine, estuarine, riverine, and man-made impoundments) and then provide two case histories in which this approach was applied: Seney National Wildlife Refuge with a restored fen system and Bosque del Apache National Wildlife Refuge where riverine processes have been simulated to restore native habitat. With adequate partnerships and administrative and political support, managers faced with degraded and/or disconnected wetland processes will be able to restore ecosystem services for society in our highly altered landscape by considering wetlands in their given hydrogeomorphic setting and temporal stage

Mallard Duckling Growth and Survival in Relation to Aquatic Invertebrates

Identification and assessment of the relative importance of factors affecting duckling growth and survival are essential for effective management of mallards on breeding areas. For each of 3 years (1993-95), we placed Fl-generation wild mallard (Anas platyrhynchos) females on experimental wetlands and allowed them to mate, nest, and rear broods for 17 days. We manipulated invertebrate densities by introducing fathead minnows (Pimephales promelas) at high densities in half of the wetlands on which broods were confined. Day- 17 body mass of surviving ducklings (n = 183) was greater for ducklings that were heavier at hatch; the difference averaged 1.7 g at day 17 for each 1.0 g at hatch (P = 0.047). Growth ratio (the proportion of body mass attained by ducklings when they were last measured relative to that predicted for wild female mallard ducklings) also was positively related to body mass at hatch (P = 0.004). Mean day-17 body mass and mean growth ratio of ducklings per brood (each adjusted for body mass at hatch) were positively related to numbers of aquatic invertebrates (Ps \u3c 0.001) and negatively related to variance in the daily minimum air temperature during the exposure period (Ps \u3c 0.020). Early growth of mallards was more sensitive to variation in numbers of invertebrates than to air temperature or biomass of invertebrates. Duckling survival was positively related to growth ratio (P \u3c 0.001). Our study provides parameter estimates that are essential for modeling growth and survival of mallard ducklings. We emphasize the need for conserving brood-rearing wetlands in the Prairie Pothole Region that are capable of supporting high densities of aquatic invertebrates

THE DRIFT SCALE HEATER TEST AT YUCCA MOUNTAIN, NEVADA

The Drift Scale Heater Test (DST) is an integral part of the program of testing and studies being conducted by the U.S. Department of Energy to evaluate the suitability of Yucca Mountain, Nevada as a site of a deep geologic repository for the permanent disposal of spent nuclear fuel and high-level nuclear waste. The DST is a large-scale, in situ thermal test to be conducted over nearly a decade in the Exploratory Studies Facility at Yucca Mountain (Figure 1). The overall objective of the DST is to acquire a more indepth understanding of the physical processes that will occur in the rock surrounding the emplaced waste. There are four principal processes of concern: thermal, mechanical, hydrological, and chemical. These processes will be intensified because of the decay heat from the emplaced waste and their interaction or coupling. An understanding of these coupled processes is essential for the assessment of the long-term (over thousands of years) performance of the repository

Semi‐quantitative characterisation of mixed pollen samples using MinION sequencing and Reverse Metagenomics (RevMet)

1. The ability to identify and quantify the constituent plant species that make up a mixed‐species sample of pollen has important applications in ecology, conservation, and agriculture. Recently, metabarcoding protocols have been developed for pollen that can identify constituent plant species, but there are strong reasons to doubt that metabarcoding can accurately quantify their relative abundances. A PCR‐free, shotgun metagenomics approach has greater potential for accurately quantifying species relative abundances, but applying metagenomics to eukaryotes is challenging due to low numbers of reference genomes. 2. We have developed a pipeline, RevMet (Reverse Metagenomics) that allows reliable and semi‐quantitative characterization of the species composition of mixed‐species eukaryote samples, such as bee‐collected pollen, without requiring reference genomes. Instead, reference species are represented only by ‘genome skims’: low‐cost, low‐coverage, short‐read sequence datasets. The skims are mapped to individual long reads sequenced from mixed‐species samples using the MinION, a portable nanopore sequencing device, and each long read is uniquely assigned to a plant species. 3. We genome‐skimmed 49 wild UK plant species, validated our pipeline with mock DNA mixtures of known composition, and then applied RevMet to pollen loads collected from wild bees. We demonstrate that RevMet can identify plant species present in mixed‐species samples at proportions of DNA ≥ 1%, with few false positives and false negatives, and reliably differentiate species represented by high versus low amounts of DNA in a sample. 4. RevMet could readily be adapted to generate semi‐quantitative datasets for a wide range of mixed eukaryote samples. Our per‐sample costs were £90 per genome skim and £60 per pollen sample, and new versions of sequencers available now will further reduce these costs

Avoiding the ask: a field experiment on altruism, empathy, and charitable giving’, mimeo

Abstract What triggers giving? We explore this in a randomized natural field experiment during the Salvation Army's annual campaign. Solicitors were at one or both of two main entrances to a supermarket, making the solicitation either easy or difficult to avoid. Additionally, solicitors were either silent, or asked "please give" to passersby. We observed over 17,000 passings over four days, and found dramatic avoidance of the solicitors, but only during a direct ask. Furthermore, asking increased donations 75%. Across all conditions, seeking the solicitor was exceedingly rare. The results do not support static views of altruism, such as inequity aversion, and instead highlight the importance of social cues and psychological features of the giver-receiver interaction. We argue that avoidance could evidence a lack of altruism or self-control strategy to deal with empathic reflexes to give. * A version of this paper constituted Trachtman's senior thesis at Harvard College, for which it was awarded the best thesis prize and summa cum laude honors. We would like to note that Trachtman took the lead responsibility in coordinating and executing the experiment. Erzo Luttmer provided exceptionally helpful advice along the way. We would also like to than