О закономерностях распределения гелия в осадочном чехле юго-востока Беларуси

In this work, microscopic and histological studies suggest that Strobilanthes crispus ethanol extract reduce azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in rats. S. crispus is considered a traditional medicine and used as an antioxidant. Its leaf contains a large amount of phenolic compounds to which its radical scavenging role is attributed and enhance its ability to eradicate oxidative stress reactions. The study was designed to determine the chemopreventive effect of S. crispus ethanol extract in vivo and in vitro by elucidating the effect of the extract on intermediate biomarkers which can be used as effective predictors of colon cancer. S. crispus was analyzed for DPPH free radical scavenging, nitric oxide (NO) and ferric acid reduction. The results indicated that S. crispus oral administration significantly inhibited colorectal carcinogenesis induced by AOM as revealed by the reduction in the number of ACF. S. crispus down-regulated the expression of PCNA, Bcl2 and beta-catenin. Additionally, it exerted a pronounced inhibitory effect on MDA and NO levels and stimulatory effect on CAT and GPx activities. These results demonstrate that S. crispus is a chemopreventive agent for colorectal cancer through the suppression of early and intermediate carcinogenic phases that may be related to its flavonoid content

Relevance of application of the Yamaguchi criteria for patients with suspected juvenile idiopathic arthritis in the absence of arthritis symptoms

Aim of the study: Systemic juvenile idiopathic arthritis (sJIA) is characterized by systemic inflammation beside arthritis. Many children may have delayed onset of arthritis. We aimed in our study to determine whether the Yamaguchi criteria (for adult onset Still’s disease) can be applied in diagnosis of sJIA, especially in absence of arthritis. Material and methods: The cross-sectional study included 30 patients diagnosed with sJIA, those patients diagnosed by the treating paediatrician with ‘definite’ sJIA (fulfilling the International League of Associations for Rheumatology [ILAR] classification criteria) or ‘suspected’ sJIA (not fulfilling the ILAR criteria). The fulfilment of the variables in both the ILAR criteria and the Yamaguchi criteria was recorded for each patient at the time of first presentation. Results: We included 16 boys and 14 girls. Ten patients were diagnosed with suspected sJIA due to the presence of typical systemic features but failed to fulfil the ILAR criteria, especially absence of arthritis in 9 of them. Yamaguchi criteria were fulfilled in a higher number of patients (23/30, 76.7%) as compared to those who fulfilled the ILAR criteria (20/30, 66.7%). All 10 patients with suspected sJIA fulfilled the Yamaguchi criteria, and 11 patients (36.7%) had delayed onset of arthritis. Overall, the 30 patients (100%) in the present study fulfilled either ILAR criteria or Yamaguchi criteria. Conclusions : There is a subgroup of patients with sJIA in whom arthritis is absent or delayed. The use of the Yamaguchi criteria in this subgroup of patients may be useful for early diagnosis and treatment of sJIA. Thus, further studies are needed to integrate supplementary criteria that increase the strength of both the Yamaguchi and the ILAR criteria

Chemopreventive Efficacy of Andrographis paniculata on Azoxymethane-Induced Aberrant Colon Crypt Foci In Vivo

Andrographis paniculata is a grass-shaped medicinal herb, traditionally used in Southeast Asia. The aim of this study was to evaluate the chemoprotective effects of A. paniculata on colorectal cancer. A. paniculata ethanol extract was tested on azoxymethane (AOM)-induced aberrant crypt foci (ACF) in vivo and in vitro. A. paniculata treated groups showed a significant reduction in the number of ACF of the treated rats. Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups. Histologically, staining showed slightly elevated masses above the surrounding mucosa with oval or slit-like orifices. Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and beta-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group. When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group. A. paniculata ethanol extract showed antioxidant and free radical scavenging activity, as elucidated by the measure of oxidative stress markers. Further, the active fractions were assessed against cell lines of CCD841 and HT29 colon cancer cells

Chemopreventive Efficacy of Andrographis paniculata on Azoxymethane-Induced Aberrant Colon Crypt Foci In Vivo

Andrographis paniculata is a grass-shaped medicinal herb, traditionally used in Southeast Asia. The aim of this study was to evaluate the chemoprotective effects of A. paniculata on colorectal cancer. A. paniculata ethanol extract was tested on azoxymethane (AOM)-induced aberrant crypt foci (ACF) in vivo and in vitro. A. paniculata treated groups showed a significant reduction in the number of ACF of the treated rats. Microscopically, ACF showed remarkably elongated and stratified cells, and depletion of the submucosal glands of AOM group compared to the treated groups. Histologically, staining showed slightly elevated masses above the surrounding mucosa with oval or slit-like orifices. Immunohistochemically, expression of proliferating cell nuclear antigen (PCNA) and beta-catenin protein were down-regulated in the A. paniculata treated groups compared to the AOM group. When colon tissue was homogenized, malondialdehyde (MDA) and nitric oxide (NO) levels were significantly decreased, whereas superoxide dismutase (SOD) activity was increased in the treated groups compared to the AOM group. A. paniculata ethanol extract showed antioxidant and free radical scavenging activity, as elucidated by the measure of oxidative stress markers. Further, the active fractions were assessed against cell lines of CCD841 and HT29 colon cancer cells

Chemopreventive effects of Strobilanthes crispus leaf extract on azoxymethane-induced aberrant crypt foci in rat colon

In this work, microscopic and histological studies suggest that Strobilanthes crispus ethanol extract reduce azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in rats. S. crispus is considered a traditional medicine and used as an antioxidant. Its leaf contains a large amount of phenolic compounds to which its radical scavenging role is attributed and enhance its ability to eradicate oxidative stress reactions. The study was designed to determine the chemopreventive effect of S. crispus ethanol extract in vivo and in vitro by elucidating the effect of the extract on intermediate biomarkers which can be used as effective predictors of colon cancer. S. crispus was analyzed for DPPH free radical scavenging, nitric oxide (NO) and ferric acid reduction. The results indicated that S. crispus oral administration significantly inhibited colorectal carcinogenesis induced by AOM as revealed by the reduction in the number of ACF. S. crispus down-regulated the expression of PCNA, Bcl2 and beta-catenin. Additionally, it exerted a pronounced inhibitory effect on MDA and NO levels and stimulatory effect on CAT and GPx activities. These results demonstrate that S. crispus is a chemopreventive agent for colorectal cancer through the suppression of early and intermediate carcinogenic phases that may be related to its flavonoid content

Identification of the main components of A. paniculata F5 by LC-MS positive and negative modes.

<p>Identification of the main components of A. paniculata F5 by LC-MS positive and negative modes.</p

Chemopreventive Efficacy of <i>Andrographis paniculata</i> on Azoxymethane-Induced Aberrant Colon Crypt Foci <i>In Vivo - Figure 5 </i>

<p>(<b>a</b>): Regulation of PCNA in the colon tissue of rats (A) Normal group (B) AOM control group (C) FU treated group, (D) 250 mg/kg <i>A. paniculata</i> treated group, and (E) 500 mg/kg <i>A. paniculata</i> treated group (100X magnification). (<b>b</b>): Regulation of PCNA protein expression in the colon tissue of normal and AOM induced rats. All values are in mean ± SEM. FU: Fluorouracil; AOM: azoxymethane.</p

Antioxidant activity of <i>A. paniculata</i> in vitro.

<p>Antioxidant activity of <i>A. paniculata</i> in vitro.</p

The histological changes of major organs (Kidney and Liver) in <i>A. paniculata</i>-treated AOM-induced cancer.

<p>They showed normal and no related toxicity in liver and kidney tissues as they are the main organs for detoxification process in the body.</p

Cross-section of the rat colon stained with hematoxylin and eosin: (A) Normal group with normal crypts, (B) AOM control group (C) FU treated group, D) 250 mg/kg <i>A. paniculata</i> treated group, (E) 500 mg/kg <i>A. paniculata</i> treated group (100X magnification).

<p>For (B), arrow indicated the elongated and slightly stratified nuclei found in AOM control group, showing depletion of mucin. For (A), (C), (D) and (E) which represented the normal cells, the arrows indicated crypts with round nuclei which are normal.</p