ABO and Rh Blood Group Distribution Among Kunbis (Maratha) population of Amravati District, Maharashtra

The present study reports the distribution of ABO and Rh blood groups among the Kunbis (Maratha) population of Amravati district. The phenotypic frequency of blood group B is observed highest (33.06) percent, O (31.04), A (27.02) and AB is lowest (08.33) percent. The phenotypic frequency of Rh negative is (04.26) percent. TheKunbis (Maratha) population shows close genetic relationship with the Gujratis

Interactions Between Spermine-Derivatized Tentacle Porphyrins And The Human Telomeric DNA G-Quadruplex

G-rich DNA sequences have the potential to fold into non-canonical G-Quadruplex (GQ) structures implicated in aging and human diseases, notably cancers. Because stabilization of GQs at telomeres and oncogene promoters may prevent cancer, there is an interest in developing small molecules that selectively target GQs. Herein, we investigate the interactions of meso-tetrakis-(4-carboxysperminephenyl)porphyrin (TCPPSpm4) and its Zn(II) derivative (ZnTCPPSpm4) with human telomeric DNA (Tel22) via UV-Vis, circular dichroism (CD), and fluorescence spectroscopies, resonance light scattering (RLS), and fluorescence resonance energy transfer (FRET) assays. UV-Vis titrations reveal binding constants of 4.7 × 10⁶ and 1.4 × 10⁷ M⁻¹ and binding stoichiometry of 2–4:1 and 10–12:1 for TCPPSpm4 and ZnTCPPSpm4, respectively. High stoichiometry is supported by the Job plot data, CD titrations, and RLS data. FRET melting indicates that TCPPSpm4 stabilizes Tel22 by 36 ± 2 °C at 7.5 eq., and that ZnTCPPSpm4 stabilizes Tel22 by 33 ± 2 °C at ~20 eq.; at least 8 eq. of ZnTCPPSpm4 are required to achieve significant stabilization of Tel22, in agreement with its high binding stoichiometry. FRET competition studies show that both porphyrins are mildly selective for human telomeric GQ vs duplex DNA. Spectroscopic studies, combined, point to end-stacking and porphyrin self-association as major binding modes. This work advances our understanding of ligand interactions with GQ DNA

Performance of VAMOS for reactions near the Coulomb barrier

Détecteur VAMOSVAMOS (VAriable MOde Spectrometer) is a large solid angle ray-tracing spectrometer employing numerical methods for reconstructing the particle trajectory. Complete identification of reaction products has been achieved by trajectory reconstruction. Equipped with a versatile detection system, VAMOS is capable of identifying reaction products from diverse reactions using beams at GANIL. The technique for trajectory reconstruction and its application for identifying reaction products are presented. The angular acceptance of the spectrometer has been studied using Monte Carlo simulation by an ion optics code. The spectrometer was coupled to the high efficiency EXOGAM γ-array to obtain γ-recoil coincidences for studying nuclei far from stability. The main features of the spectrometer as well as some results applied to experiments in deep inelastic collisions are described

Heterosis in single cross inter and intra-specific hybrids of Desi cotton (Gossipium arboreum and G. herbaceaum) for their seed cotton yield, fibre quality and seed oil content

The present investigation was carried out to assess the expression of per se performance and heterotic effect for fibre quality and seed oil content besides seed cotton yield, studied involving ten desi cotton (Gossipium arboreum and G. herbaceaum) genotypes and their 45 cross combinations in half diallel analysis. F-1 hybrids GBhv-282 x G 27 (67.36%), GBhv- 287 x 824 (58.14%), GBhv- 282 x GAM- 173 (35.00%), GBhv- 286 x G 27 (20.50%), and GBhv- 283 x 824 (18.75%) recorded highest per se performance and significant positive standard heterosis while the maximum heterobeltiosis for seed cotton yield per plant was exhibited by the hybrid GBhv- 287 x 824(155.60 %) followed by GBhv- 282 x G 27 (151.29%) and GBhv- 282 x GAM- 173 (130.30%). Similar trend of heterosis for numbers of boll per plant were observed in above hybrids. For fibre quality traits none of the cross showed consistent high performance for all the characters. Cross GBhv- 283 x 824 was exhibited high standard heterosis for 2.5 % span length, fibre strength, fibre elongation percentage as well as for short fibre index (SFI) while cross GBhv- 286 x 824 were promising for 2.5 per cent span length, fibre strength and fibre fineness. In case of oil content intraarboreum crosses resulted as better crosses and among them cross combination 824 x GAM- 173 was best. Desi cotton hybrids are having lower fibre quality and yield. So, improvement for yield and fibre quality of diploid native varieties through heterosis breeding provided better hybrids for rainfed farming