Expression of aldehyde dehydrogenase family 1, member A3 in glycogen trophoblast cells of the murine placenta

Introduction: Retinoic acid (RA) signaling is a well known regulator of trophoblast differentiation and placental development, and maternal decidual cells are recognized as the source of much of this RA. We explored possible trophoblast-derived sources of RA by examining the expression of RA synthesis enzymes in the developing mouse placenta, as well as addressed potential sites of RA action by examining the ontogeny of gene expression for other RA metabolizing and receptor genes. Furthermore, we investigated the effects of endogenous RA production on trophoblast differentiation

Δ9-tetrahydrocannabinol exposure during rat pregnancy leads to symmetrical fetal growth restriction and labyrinth-specific vascular defects in the placenta.

1 in 5 women report cannabis use during pregnancy, with nausea cited as their primary motivation. Studies show that (-)-△9-tetrahydrocannabinol (Δ9-THC), the major psychoactive ingredient in cannabis, causes fetal growth restriction, though the mechanisms are not well understood. Given the critical role of the placenta to transfer oxygen and nutrients from mother, to the fetus, any compromise in the development of fetal-placental circulation significantly affects maternal-fetal exchange and thereby, fetal growth. The goal of this study was to examine, in rats, the impact of maternal Δ9-THC exposure on fetal development, neonatal outcomes, and placental development. Dams received a daily intraperitoneal injection (i.p.) of vehicle control or Δ9-THC (3 mg/kg) from embryonic (E)6.5 through 22. Dams were allowed to deliver normally to measure pregnancy and neonatal outcomes, with a subset sacrificed at E19.5 for placenta assessment via immunohistochemistry and qPCR. Gestational Δ9-THC exposure resulted in pups born with symmetrical fetal growth restriction, with catch up growth by post-natal day (PND)21. During pregnancy there were no changes to maternal food intake, maternal weight gain, litter size, or gestational length. E19.5 placentas from Δ9-THC-exposed pregnancies exhibited a phenotype characterized by increased labyrinth area, reduced Epcam expression (marker of labyrinth trophoblast progenitors), altered maternal blood space, decreased fetal capillary area and an increased recruitment of pericytes with greater collagen deposition, when compared to vehicle controls. Further, at E19.5 labyrinth trophoblast had reduced glucose transporter 1 (GLUT1) and glucocorticoid receptor (GR) expression in response to Δ9-THC exposure. In conclusion, maternal exposure to Δ9-THC effectively compromised fetal growth, which may be a result of the adversely affected labyrinth zone development. These findings implicate GLUT1 as a Δ9-THC target and provide a potential mechanism for the fetal growth restriction observed in women who use cannabis during pregnancy

Two-photon final states in peripheral heavy ion collisions

We discuss processes leading to two photon final states in peripheral heavy ion collisions at RHIC. Due to the large photon luminosity we show that the continuum subprocess $\gamma \gamma \to \gamma \gamma$ can be observed with a large number of events. We study this reaction when it is intermediated by a resonance made of quarks or gluons and discuss its interplay with the continuum process, verifying that in several cases the resonant process ovewhelms the continuum one. It is also investigated the possibility of observing a scalar resonance (the $\sigma$ meson) in this process. Assuming for the $\sigma$ the mass and total decay width values recently reported by the E791 Collaboration we show that RHIC may detect this particle in its two photon decay mode if its partial photonic decay width is of the order of the ones discussed in the literature.Comment: 10 pages, 8 figure

Relating the Quark and Gluon Condensates Through the QCD Vacuum Energy

Using the Cornwall-Jackiw-Tomboulis effective potential for composite operators we compute the QCD vacuum energy as a function of the dynamical quark and gluon propagators, which are related to their respective condensates as predicted by the operator product expansion. The identification of this result to the vacuum energy obtained from the trace of the energy-momentum tensor allow us to study the gluon self-energy, verifying that it is fairly represented in the ultraviolet by the asymptotic behavior predicted by the operator product expansion, and in the infrared it is frozen at its asymptotic value at one scale of the order of the dynamical gluon mass. We also discuss the implications of this identity for heavy and light quarks. For heavy quarks we recover, through the vacuum energy calculation, the relation m_{f} \sim - {1/12} \gc obtained many years ago with QCD sum rules.Comment: 21 pages, LaTeX, final version to appear in Physical Review

Effects of the 2003 European heatwave on the Central Mediterranean Sea: surface fluxes and the dynamical response

International audienceThe effects of the 2003 European heatwave on the sea surface layer of the Central Mediterranean were studied using a regional 3-D ocean model. The model was used to simulate the period 2000 to 2004 and its performance was validated using remotely-sensed and in situ data. Analysis of the results focused on changes in the Sea Surface Temperature (SST) and on changes to the surface and sub-surface current field. This permitted us to identify and quantify the anomalies of atmospheric and sea surface parameters that accompanied the heatwave. The dominant annual cycle in each variable was first removed and a wavelet analysis then used to locate anomalies in the time-frequency domain. We found that the excess heating affecting the sea surface in the summer of 2003 was related to a significant increase in air temperature, a decrease in wind stress and reduction of all components of the upward heat flux. The monthly averages of the model SST were found to be in good agreement with remotely-sensed data during the period studied, although the ocean model tended to underestimate extreme events. The spatial distribution of SST anomalies as well as their time-frequency location was similar for both the remotely-sensed and model temperatures. We also found, on the basis of the period of the observed anomaly, that the event was not limited to the few summer months of 2003 but was part of a longer phenomenon. Both the model results and experimental data suggest the anomalous heating mainly affected the top 15 m of ocean and was associated with strong surface stratification and low mixing. The skill of the model to reproduce the sub-surface hydrographic features during the heatwave was checked by comparison with temperature and salinity measurements. This showed that the model was generally in good agreement with observations. The model and observations showed that the anomalous warming also modified the currents in the region, most noticeably the Atlantic Ionian Stream (AIS) and the Atlantic Tunisian Current (ATC). The AIS was reduced in intensity and showed less meandering, mainly due to the reduced density gradient and low winds, while the ATC was enhanced in strength, the two currents appearing to modulate each other in order to conserve the total transport of Modified Atlantic Water

Validation of the German Revised Addenbrooke's Cognitive Examination for Detecting Mild Cognitive Impairment, Mild Dementia in Alzheimer's Disease and Frontotemporal Lobar Degeneration

Background/Aims: The diagnostic accuracy of the German version of the revised Addenbrooke's Cognitive Examination (ACE-R) in identifying mild cognitive impairment (MCI), mild dementia in Alzheimer's disease (AD) and mild dementia in frontotemporal lobar degeneration (FTLD) in comparison with the conventional Mini Mental State Examination (MMSE) was assessed. Methods: The study encompasses 76 cognitively healthy elderly individuals, 75 patients with MCI, 56 with AD and 22 with FTLD. ACE-R and MMSE were validated against an expert diagnosis based on a comprehensive diagnostic procedure. Statistical analysis was performed using the receiver operating characteristic method and regression analyses. Results: The optimal cut-off score for the ACE-R for detecting MCI, AD, and FTLD was 86/87, 82/83 and 83/84, respectively. ACE-R was superior to MMSE only in the detection of patients with FTLD {[}area under the curve (AUC): 0.97 vs. 0.92], whilst the accuracy of the two instruments did not differ in identifying MCI and AD. The ratio of the scores of the memory ACE-R subtest to verbal fluency subtest contributed significantly to the discrimination between AD and FTLD (optimal cut-off score: 2.30/2.31, AUC: 0.77), whereas the MMSE and ACE-R total scores did not. Conclusion: The German ACE-R is superior to the most commonly employed MMSE in detecting mild dementia in FTLD and in the differential diagnosis between AD and FTLD. Thus it might serve as a valuable instrument as part of a comprehensive diagnostic workup in specialist centres/clinics contributing to the diagnosis and differential diagnosis of the cause of dementia. Copyright (C) 2010 S. Karger AG, Base

Photon-Photon and Pomeron-Pomeron Processes in Peripheral Heavy Ion Collisions

We estimate the cross sections for the production of resonances, pion pairs and a central cluster of hadrons in peripheral heavy-ion collisions through two-photon and double-pomeron exchange, at energies that will be available at RHIC and LHC. The effect of the impact parameter in the diffractive reactions is introduced, and imposing the condition for realistic peripheral collisions we verify that in the case of very heavy ions the pomeron-pomeron contribution is indeed smaller than the electromagnetic one. However, they give a non-negligible background in the collision of light ions. This diffractive background will be more important at RHIC than at LHC.Comment: 22 pages, 1 Postscript figures, 4 tables, to appear in Phys. Rev.

Freezing of the QCD coupling constant and solutions of Schwinger-Dyson equations

We compare phenomenological values of the frozen QCD running coupling constant ($\alpha_s$) with two classes of solutions obtained through nonperturbative Schwinger-Dyson equations. We use these same solutions with frozen coupling constants as well as their respective nonperturbative gluon propagators to compute the QCD prediction for the asymptotic pion form factor. Agreement between theory and experiment on $\alpha_s(0)$ and $F_\pi (Q^2)$ is found only for one of the solutions Schwinger-Dyson equations.Comment: 6 pages, 4 figures, RevTeX, axodra

An ontology for major histocompatibility restriction

BACKGROUND: MHC molecules are a highly diverse family of proteins that play a key role in cellular immune recognition. Over time, different techniques and terminologies have been developed to identify the specific type(s) of MHC molecule involved in a specific immune recognition context. No consistent nomenclature exists across different vertebrate species. PURPOSE: To correctly represent MHC related data in The Immune Epitope Database (IEDB), we built upon a previously established MHC ontology and created an ontology to represent MHC molecules as they relate to immunological experiments. DESCRIPTION: This ontology models MHC protein chains from 16 species, deals with different approaches used to identify MHC, such as direct sequencing verses serotyping, relates engineered MHC molecules to naturally occurring ones, connects genetic loci, alleles, protein chains and multi-chain proteins, and establishes evidence codes for MHC restriction. Where available, this work is based on existing ontologies from the OBO foundry. CONCLUSIONS: Overall, representing MHC molecules provides a challenging and practically important test case for ontology building, and could serve as an example of how to integrate other ontology building efforts into web resources. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13326-016-0045-5) contains supplementary material, which is available to authorized users