Comparative expression profiling and sequence characterization of ATP1A1 gene associated with heat tolerance in tropically adapted cattle

Climate change is an imminent threat to livestock production. One adaptation strategy is selection for heat tolerance. While it is established that the ATP1A1 gene and its product play an important role in the response to many stressors, there has been no attempt to characterize the sequence or to perform expression profiling of the gene in production animals. We undertook a field experiment to compare the expression profiles of ATP1A1 in heat-tolerant Vechur and Kasaragod cattle (Bos taurus indicus) with the profile of a heat-susceptible crossbreed (B. t. taurus × B. t. indicus). The cattle were exposed to heat stress while on pasture in the hot summer season. The environmental stress was quantified using the temperature humidity index (THI), while the heat tolerance of each breed was assessed using a heat tolerance coefficient (HTC). The ATP1A1 mRNA of Vechur cattle was amplified from cDNA and sequenced. The HTC varied significantly between the breeds and with time-of-day (p < 0.01). The breed–time-of-day interaction was also significant (p < 0.01). The relative expression of ATP1A1 differed between heat-tolerant and heat-susceptible breeds (p = 0.02). The expression of ATP1A1 at 08:00, 10:00 and 12:00, and the breed–time-of-day interaction, were not significant. The nucleotide sequence of Vechur ATP1A1 showed 99% homology with the B. t. taurus sequence. The protein sequence showed 98% homology with B. t. taurus cattle and with B. grunniens (yak) and 97.7% homology with Ovis aries (sheep). A molecular clock analysis revealed evidence of divergent adaptive evolution of the ATP1A1 gene favoring climate resilience in Vechur cattle. These findings further our knowledge of the relationship between the ATP1A1 gene and heat tolerance in phenotypically incongruent animals. We propose that ATP1A1 could be used in marker assisted selection (MAS) for heat tolerance

Influence of Incomplete Fusion Reaction on Complete Fusion Below 10 Mev/ Nucleon Energies

An attempt has been made in the present work to provide an ample opportunity to explore the information about the influence of incomplete fusion (ICF) reaction dynamics on complete fusion in heavy ion induced nuclear reactions. excitation functions for several evaporation residues produced in the interaction of projectile 16O with target 175lu have been measured over the wide projectile energy range ≈ 70-100 MeV. the recoil-catcher activation technique followed by the offline γ-ray spectroscopy has been used for the present measurements. In case of precursor decay, we have made use of Cavinato et al. formulation to calculate the independent cross-section of the identified residues. the measured efs are compared with theoretical predictions of statistical model code PACE-2 and any enhancement in the measured cross-section from theoretical prediction may be due to ICF reaction process. An attempt has been made to estimate the ICf contribution of the cross-section from the measured excitation function data and the dependence of ICf cross-section on projectile energy

A Comparative Study of IP Versions 4, 5, and 6

This research examines the Internet Protocol (IP) versions 4, 5, and 6, as well as the differences between them and which protocol is more suitable for the future of the internet, among other things. Through this research, we have established the most advantageous characteristics of these protocols, as well as the specific elements that each protocol uses to allow the internet network to operate at maximum capacity. The main aim of this study is to discover which of the internet protocols, IPv4, IPv5, or IPv6, is the most widely used. IPv4 is the most widely used protocol, followed by IPv5. The most essential elements of getting a more relevant job on the internet network are highlighted in this article. It all comes down to how IP protocols operate and what they accomplish

Study of break-up fusion process from forward recoil range distribution measurement

540-543In the present work, the break-up fusion or incomplete fusion (ICF) process has been studied from the forward recoil range distribution measurement for 16O + 175Lu system at ≈ 96 MeV energy. The measured forward recoil range distributions are analyzed in the framework of code SRIM. The present analysis shows clearly the role of linear momentum transfer and also the break-up of projectile 16O into its fragments (12C + 4He and/or 8Be + 8Be). Any systematic trend is not observed with the target deformation parameter (β2) dependent study of ICF. It is observed that projectile structure also affects the ICF dynamics. The projectile α-Q-value is found to be a suitable parameter which explains effectively the observed projectile structure effect on ICF

Natural Medicine a Promising Candidate in Combating Microbial Biofilm

Studies on biofilm-related infections are gaining prominence owing to their involvement in most clinical infections and seriously threatening global public health. A biofilm is a natural form of bacterial growth ubiquitous in ecological niches, considered to be a generic survival mechanism adopted by both pathogenic and non-pathogenic microorganisms and entailing heterogeneous cell development within the matrix. In the ecological niche, quorum sensing is a communication channel that is crucial to developing biofilms. Biofilm formation leads to increased resistance to unfavourable ecological effects, comprising resistance to antibiotics and antimicrobial agents. Biofilms are frequently combated with modern conventional medicines such as antibiotics, but at present, they are considered inadequate for the treatment of multi-drug resistance; therefore, it is vital to discover some new antimicrobial agents that can prevent the production and growth of biofilm, in addition to minimizing the side effects of such therapies. In the search for some alternative and safe therapies, natural plant-derived phytomedicines are gaining popularity among the research community. Phytomedicines are natural agents derived from natural plants. These plant-derived agents may include flavonoids, terpenoids, lectins, alkaloids, polypeptides, polyacetylenes, phenolics, and essential oils. Since they are natural agents, they cause minimal side effects, so could be administered with dose flexibility. It is vital to discover some new antimicrobial agents that can control the production and growth of biofilms. This review summarizes and analyzes the efficacy characteristics and corresponding mechanisms of natural-product-based antibiofilm agents, i.e., phytochemicals, biosurfactants, antimicrobial peptides, and their sources, along with their mechanism, quorum sensing signalling pathways, disrupting extracellular matrix adhesion. The review also provides some other strategies to inhibit biofilm-related illness. The prepared list of newly discovered natural antibiofilm agents could help in devising novel strategies for biofilm-associated infections

Natural Medicine a Promising Candidate in Combating Microbial Biofilm

Studies on biofilm-related infections are gaining prominence owing to their involvement in most clinical infections and seriously threatening global public health. A biofilm is a natural form of bacterial growth ubiquitous in ecological niches, considered to be a generic survival mechanism adopted by both pathogenic and non-pathogenic microorganisms and entailing heterogeneous cell development within the matrix. In the ecological niche, quorum sensing is a communication channel that is crucial to developing biofilms. Biofilm formation leads to increased resistance to unfavourable ecological effects, comprising resistance to antibiotics and antimicrobial agents. Biofilms are frequently combated with modern conventional medicines such as antibiotics, but at present, they are considered inadequate for the treatment of multi-drug resistance; therefore, it is vital to discover some new antimicrobial agents that can prevent the production and growth of biofilm, in addition to minimizing the side effects of such therapies. In the search for some alternative and safe therapies, natural plant-derived phytomedicines are gaining popularity among the research community. Phytomedicines are natural agents derived from natural plants. These plant-derived agents may include flavonoids, terpenoids, lectins, alkaloids, polypeptides, polyacetylenes, phenolics, and essential oils. Since they are natural agents, they cause minimal side effects, so could be administered with dose flexibility. It is vital to discover some new antimicrobial agents that can control the production and growth of biofilms. This review summarizes and analyzes the efficacy characteristics and corresponding mechanisms of natural-product-based antibiofilm agents, i.e., phytochemicals, biosurfactants, antimicrobial peptides, and their sources, along with their mechanism, quorum sensing signalling pathways, disrupting extracellular matrix adhesion. The review also provides some other strategies to inhibit biofilm-related illness. The prepared list of newly discovered natural antibiofilm agents could help in devising novel strategies for biofilm-associated infections

Evaluation of physiological traits, yield and yield components at two growth stages in 10 durum wheat lines grown under rainfed conditions in Southern Syria

Water stress, which limits the distribution and productivity of durum wheat (Triticum durum Desf.) in the Mediterranean region, is also considered to be a major factor reducing yield in semiarid regions. Improving drought resistance is thus an important objective in plant breeding programs for rainfed agriculture. The current study was carried out to identify drought-tolerant durum wheat lines among 10 lines and one variety (Douma1, the control) in the first and second settlement zones in the Southern part of Syria and to recognize the most important physiological parameters associated with drought tolerance. Membrane stability index, chlorophyll (chl) content, relative water content and chl fluorescence were recorded at the vegetative and anthesis stages, as were yield and yield components. Data recorded at vegetative and anthesis stages in both zones showed that there were significant differences between all lines growing in the first and second settlement zones and that all characters in the second zone were significantly lower than those in the first zone. Line 1 was superior to Douma1 in terms of membrane stability index, relative water content, chl content and chl florescence, also showing better yield and higher total plant biomass, tiller number/m2, 1000 grain weight and grain number/ear than the control. The ability of wheat cultivars to perform reasonably well in variable rainfall and water-stressed environments is an important trait since it allows for stable production under drought stress. Moreover, prior to genetic manipulation, it is important to characterize the physiological parameters of known drought-tolerant or drought-sensitive wheat cultivars with the objective of better understanding their physiological responses under drought