Effects of physical exercise in biochemical parameters and dorsolateral prostate lesions: data from a rat model of prostate cancer

Background: Prostate cancer (PCa) is among the most prevalent cancers worldwide. Physical exercise is widely recognized due to its beneficial effects. This study aimed to evaluate the effects of physical exercise on biochemical pa- rameters and in dorsolateral prostate lesions in a rat model of PCa. Materials and Methods: Ninety-five male Wistar Unilever rats were randomly divided into eight groups sacrificed at 35 (groups I) or 61 weeks of age (groups II): control sedentary groups (Cont+Sed I (n = 10); Cont+Sed II (n = 10)); induced sedentary group (PCa+Sed I (n = 10); PCa+Sed II (n = 15)); control exercised groups (Cont+EX I (n = 10); Cont+EX II (n = 10)) and induced exercised groups (PCa+EX I (n = 10); PCa+EX II (n = 20)). All procedures were approved (DGAV, no. 021326). Animals from exercised groups started the exer- cise program in a treadmill at 8 weeks of age, for 28 weeks or 53 weeks. The animals were trained 5 days/week, 60 min per day. Prostate lesions were induced at 12 weeks of age, with sequential administration of flutamide, testosterone propion- ate and N-methyl-N-nitrosourea, and subcutaneous implants of crystalline testosterone. Animals were sacrificed at 35 or 61 weeks of age. Peripheral blood of all animals was col- lected by intracardiac puncture. A complete necropsy was performed. The dorsolateral prostate tissues sections were processed for histological analysis. Data were analysed using SPSS 25. p 0.05). Dorsolateral prostate lesions were classified as dysplasia, prostatic intraep- ithelial neoplasia (PIN) and microinvasive carcinoma. The number of prostate lesions was higher in animals from groups II than in those from groups I, mainly in PCa+Sed II animals when compared with PCa+Sed I (p 0.05). Conclusions: Overall, the animals sacrificed at 61 weeks of age developed more dorsolateral prostate lesions than ani- mals sacrificed at 35 weeks of age, which may be related to a longer testosterone exposure

The influence of physical exercise on oestrogen and androgen receptor expression in a chemically and hormonally-induced rat model of prostate cancer

Background: Oestrogen (ER) and androgen (AR) recep- tors play an important role in normal prostate development and are also implied in prostate cancer (PCa) development. Several studies suggested that physical activity may decrease the risk of PCa development and also changes sexual hor- mones and their receptors. This study aimed to evaluate the effects of physical exercise on ERα and AR expression in a rat model of chemically and hormonally-induced PCa. Materials and Methods: Fifty-five male Wistar Unilever rats of 12 weeks of age were randomly divided into four groups: control sedentary (n = 10), control exercised (n = 10), induced sedentary (n = 15) and induced exercised (n = 20). Animals from exercised groups started the exercise training in a treadmill (Treadmill Control LE 8710, Harvard Apparatus, USA), at the age of 8 weeks, for 35 weeks (5 days/week). The protocol for PCa induction started at 12 weeks of age and consisted of sequential administration of flutamide (50 mg/kg, TCI Chemicals), testosterone propion- ate (100 mg/kg, TCI Chemicals) and N-methyl-N-nitrosourea (30 mg/kg, Isopac®, Sigma Chemical Co.), followed by sub- cutaneous implants of crystalline testosterone. Animals were sacrificed at 61 weeks of age and a complete necropsy was performed. All experiments were approved by DGAV (no. 021326). Antibodies for Erα (1:500, clone 6F11, Novocastra) and AR (clone PG21, Merck Millipore) were used for the immunohistochemical study. The staining extension was evaluated in normal prostate tissue and in dorsolateral pros- tate lesions (hyperplasia, dysplasia, prostatic intraepithelial neoplasia (PIN) and microinvasive carcinoma) and assessed to five levels (0%, 75%), con- sidering the extension of immunopositive tissue. Data was analysed with SPSS 25.Results: The normal prostate tissue and dorsolateral prostate lesions of animals from all groups were immunopositive for Erα and AR. However, the groups showed high immunoposi- tivity for AR and low positivity for Erα ( 0.05). The malignant lesions (PIN and microinvasive carcinoma) showed lower AR expression when compared with normal prostate tissue in all groups. Conclusions: As expected, the AR expression was lower in malignant lesions. Inversely to that reported in other studies, the exercise training did not modify the ERα and AR expres- sion, which may be related to the duration and type of exer- cise performed

Analysis of Conceptual Definitions of Agribusiness Using the Conceptual Bibliographic Method

Purpose: Agribusiness is a phenomenon of interest to science from several aspects, and this multiplicity of aspects taken simultaneously is a new way of trying to understand and explain it. Specific, unidisciplinary studies still predominate on a single element of agribusiness, such as finance, marketing, and production, among countless others. However, the trend is towards multifactorial studies because agribusiness is a phenomenon that cannot be classified solely as industry, commerce, or service or as a primary, secondary, or tertiary sector. Consolidated agribusiness is all of these things simultaneously, whose reality is delimited by science based on its conceptual definitions. Therefore, the definitions must be analyzed so that the current stage of scientific knowledge about the phenomenon can be understood, especially its limits, characteristics, and dynamics, which are the purposes of this study.   Theoretical Structure: The theoretical architecture of this study consisted of seeking to understand the phenomenon of agribusiness with socio-economic development, which is its most evident externality. It was carried out interdisciplinary, highlighting its integral reality based on what appears and can be identified in the conceptual definitions available in scientific publications. The justification for this procedure was the need to understand agribusiness from multiple scientific views so that its various aspects could be understood.   Design/Methodology/Approach: This study aimed to analyze the conceptual definitions of agribusiness in the scientific literature in studies published between 2015 and 2023. To this end, it established three guiding questions, which sought to identify the frontiers of knowledge about the phenomenon, its main attributes, and what logical scheme it is possible to find among its main defining characteristics. For this, the conceptual bibliographic method was used, which consists of formulating a problem and its breakdown into a response pattern, collecting data in scientific databases, organizing and analyzing data based on semantic resources, and generating responses from the diagrammatic layout of the data organization.   Findings: The results showed the existence of nine approaches to agribusiness (set, sum, activity, science, practice, configuration, industry, process, and transformation) and eight groups of attributes (transnational activities, commercialization, distribution logistics, supply logistics, companies, production, services, and agroecosystem). These discoveries allowed us to understand that agribusiness has an evolutionary dynamic that begins with the professionalization of the rural output and culminates with the irradiation of the reach of this production to transnational borders. Research, Practical, and Social Implications: These findings fundamentally affect how agribusiness is viewed. In Brazil, in particular, the prevalent mentality tends to distinguish agribusiness as a unique and exclusive form of large rural enterprises, almost all of which are already globalized, excluding small enterprises from this coverage, almost always classified as family farming. Suppose the family farmer establishes himself as an enterprise. In that case, he often receives the entire discriminatory range intended for the large agribusiness enterprise, discouraging his professionalization, an essential corollary of the germ of agribusiness. Professionalization involves replacing improvised methods of producing and managing the enterprise with rational aspects originating from science and introducing scientifically subsidized techniques and technologies.   Originality/Value: The main contribution of this study to science was the construction of an evolutionary dynamic of agribusiness, which begins with the professionalization of rural production and culminates with globalization. These findings suggest that the family farming stage needs to be broken so that the benefits of professionalization can lead to improved quality of life and desired socioeconomic development for producers and partners

Aberrant p15, p16, p53, and DAPK Gene Methylation in Myelomagenesis: Clinical and Prognostic Implications

BACKGROUND: Aberrant DNA methylation is considered a crucial mechanism in the pathogenesis of monoclonal gammopathies. We aimed to investigate the contribution of hypermethylation of 4 tumor suppressor genes to the multistep process of myelomagenesis. METHODS: The methylation status of p15, p16, p53, and DAPK genes was evaluated in bone marrow samples from 94 patients at diagnosis: monoclonal gammopathy of uncertain significance (MGUS) (n = 48), smoldering multiple myeloma (SMM) (n = 8) and symptomatic multiple myeloma (MM) (n = 38), and from 8 healthy controls by methylation-specific polymerase chain reaction analysis. RESULTS: Overall, 63% of patients with MM and 39% of patients with MGUS presented at least 1 hypermethylated gene (P < .05). No aberrant methylation was detected in normal bone marrow. The frequency of methylation for individual genes in patients with MGUS, SMM, and MM was p15, 15%, 50%, 21%; p16, 15%, 13%, 32%; p53, 2%, 12,5%, 5%, and DAPK, 19%, 25%, 39%, respectively (P < .05). No correlation was found between aberrant methylation and immunophenotypic markers, cytogenetic features, progression-free survival, and overall survival in patients with MM. CONCLUSIONS: The current study supports a relevant role for p15, p16, and DAPK hypermethylation in the genesis of the plasma cell neoplasm. DAPK hypermethylation also might be an important step in the progression from MGUS to MM.info:eu-repo/semantics/publishedVersio

Análise das internações hospitalares por diarreia e gastroenterite infecciosa no Estado de Goiás entre 2008 a 2018

Introdução: A gastroenterite caracteriza-se por inflamação do revestimento do estômago, intestino delgado e cólon, sendo, na maioria dos casos, de origem infecciosa (viral, bacteriana, parasitária principalmente) e curso autolimitado. Esta patologia possui forte relação com a precariedade dos hábitos de higiene da população. As manifestações clínicas, em geral, são cólicas abdominais, náuseas, vômitos e diarreia persistente de início súbito. O diagnóstico envolve anamnese e propedêutica fecal. O tratamento é sintomático, embora em algumas infecções parasitárias e bacterianas há necessidade de tratamento anti-infeccioso específico. A gastroenterite cursa com perda hidroeletrolítica importante, sendo importante causa de hospitalizações e custos para saúde pública. Objetivo: Analisar o número de internações hospitalares/ano por diarreia e gastroenterite infecciosa presumível no Estado de Goiás, no período de 2008 a 2018, considerando as variáveis idade (menor de um ano à 80 anos) e sexo. Material e método: Foi realizado estudo descritivo, retrospectivo e longitudinal. Analisando dados do Sistema de Internações Hospitalares do Sistema Único de Saúde (SIH/SUS), envolvendo o número de internações por diarreia e gastroenterite infecciosa presumível por ano no Estado de Goiás. Resultados: Foram notificadas 62.435 internações. As faixas etárias de casos registrados foram: crianças menores de um ano de idade com 5.130 (8,21%) casos, de 1-4 anos 15.453 (24,75%), e 5-9 anos 6.999 (11,21%). Em relação ao sexo, houve maior prevalência das afecções entre as mulheres, correspondendo 33.252 (53,26%) casos, em comparação com 29.183(46,74%) casos no sexo masculino. De forma geral, a quantidade total de internações variou de 8.206 em 2008 para 3.151 em 2018, refletindo uma redução de 61,6%. Conclusão: Nossa análise demonstra que houve uma redução nas hospitalizações causadas por gastroenterite infecciosa no Estado de Goiás no decorrer da última década em todas as faixas etárias. Observou-se que o número de casos foram significativamente maiores em mulheres e crianças entre 1-4 anos de idade. Destaca-se ainda a redução de 61,6% do número total de casos de diarreia e gastroenterite no período analisado, refletindo a importância das políticas de saneamento básico, inferindo a melhora dos hábitos de higiene da população. Assim concluímos a relevância de estudos de prevalência das gastroenterites na promoção de medidas de saúde pública com intuito de reduzir cada vez mais os casos de dessa afecção e as consequentes internações

Correlation between avascular necrosis and early stabilization of proximal femoral fractures in childhood

OBJETIVE: This study was developed with the main purpose of evaluating treatment results of proximal femoral fractures in a series of cases. We sought to observe the influence of the most frequent complications on the final results after a minimum follow-up of 2 years. We have especially considered the relationship between development of avascular necrosis and time between the accident and therapeutic intervention. METHOD: We retrospectively studied proximal femoral fractures in 29 patients under 14 years of age from 1988 to 2007. The following parameters were analyzed: sex, age, mechanism of injury, fracture classification (Delbet), treatment, complications (pseudoarthrosis, coxa vara, leg length discrepancy and avascular necrosis), time for surgery, and results (Ratliff). Statistical analysis was performed according to the descriptive evaluation of each parameter by using Fisher's exact test. RESULTS: Five (17.2%) patients had avascular necrosis, 3 of whom (60.0%) were older than 10 years of age. Seventy-three point three percent of patients treated in the first 24 hours showed good results. The most common cause of fracture was traffic accident (44.8%). The best results were observed in patients who were treated surgically; 41.4% developed some type of complication. CONCLUSIONS: Among the 29 patients treated, 58.6% had good, 27.6% had regular and 13.8% had poor results according to Ratliff criteria. When conservative treatment was applied, only 17.0% had good results, while surgical intervention results were 69.3% good. In addition, we obtained 73.3% good results when surgery was performed within the first 24 hours and only 42.8% good results in patients submitted to surgery after this period. Patients operated in the first 24 hours developed avascular necrosis in 13.3% of cases, while 21.4% of those operated after that period developed this complication.OBJETIVO: Desenvolvemos este trabalho, com o intuito de avaliar o resultado do tratamento de pacientes portadores de fraturas do fêmur proximal, em uma série de casos. Procuramos observar a influência das complicações mais prevalentes nos resultados finais após o mínimo de dois anos de seguimento. Correlacionamos especialmente a instalação da necrose avascular e o tempo entre o acidente e a instituição da terapêutica. MÉTODOS: Estudamos, retrospectivamente, 29 pacientes com fraturas da extremidade proximal do fêmur, com idade inferior a 14 anos entre 1988 e 2007. Analisamos as seguintes variáveis: sexo, idade, mecanismo de trauma, classificação da fratura (Delbet), tratamento realizado, complicações (pseudartrose, deformidade em varo, anisomelia e necrose avascular), tempo para cirurgia e resultado (Ratliff). Obtivemos uma análise descritiva individual de cada variável. Os testes foram utilizados de acordo com a adequação das premissas de normalidade e para avaliação utilizamos o teste exato de Fisher. RESULTADOS: Obtivemos cinco (17,2%) pacientes com necrose avascular sendo três (60,0%) com idade superior a 10 anos; 73,3% dos pacientes tratados nas primeiras 24 horas apresentaram bons resultados; a causa mais comum de fratura foi acidente automobilístico (44,8%); os melhores resultados foram observados nos pacientes tratados cirurgicamente; 41,4% evoluíram com algum tipo de complicação. CONCLUSÕES: Entre os 29 pacientes tratados, segundo os critérios de Ratliff, obtivemos 58,6% de bons, 27,6% de regulares e 13,8% de maus resultados. Quando aplicado o tratamento incruento, obtivemos apenas 17,0% de bons resultados, enquanto que após o tratamento cirúrgico obtivemos 69,3%. Da mesma forma, observamos que houve 73,3% de bons resultados quando a cirurgia foi realizada nas primeiras 24 horas e apenas 42,8% nos pacientes submetidos à intervenção terapêutica após este período. Pacientes submetidos à cirurgia nas primeiras 24 horas evoluíram com necrose da cabeça do fêmur em 13,3%, enquanto os que foram operados após este período tiveram esta complicação em 21,4% dos casos.Universidade Federal de São Paulo (UNIFESP) Departamento de Ortopedia e TraumatologiaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaUniversidade Federal de São Paulo (UNIFESP) Departamento de Ortopedia e Traumatologia Clínica da Disciplina de Ortopedia PediátricaUniversidade Federal de São Paulo (UNIFESP) Departamento de Ortopedia e Traumatologia Disciplina de Ortopedia PediátricaUNIFESP, Depto. de Ortopedia e TraumatologiaUNIFESP, EPMUNIFESP, Depto. de Ortopedia e Traumatologia Clínica da Disciplina de Ortopedia PediátricaUNIFESP, Depto. de Ortopedia e Traumatologia Disciplina de Ortopedia PediátricaSciEL

Destreza do modelo climático regionalizado ETA-HADGEM2-ES para precipitação em bacia da Mata Atlântica.

Projeções de modelos climáticos regionalizados são informações-chave nos estudos de impacto de mudanças climáticas em Hidrologia. Essas projeções são, em geral, afetadas por erros sistemáticos ou vieses que comprometem a destreza do modelo. O trabalho avalia a destreza do modelo regionalizado EtaHadGEM2-ES na bacia Guapi- Macacu, RJ, para médias mensais de chuva ao longo do ano e no trimestre mais chuvoso (DJF: dezembro, janeiro e fevereiro). Foram calculados os valores de viés linear (mm) e relativo (%) para cada pixel na região de estudo pela diferença entre as médias mensais do período 1976 - 2005 das projeções retrospectivas (hindcasts) do modelo avaliado e os respectivos valores observados, obtidos a partir da base de dados globais interpolados CRU. As médias dos totais mensais de chuva foram usadas para avaliar a reprodução do ciclo anual pelo modelo. O modelo EtaHadGEM2-ES reproduz razoavelmente o ciclo anual de chuva na bacia, com tendência de superestimação do total anual e subestimação do trimestre DJF. A chuva acumulada na bacia em DJF (médias de 1976 - 2005) variou de 329 a 743 mm (CRU) e 129 a 890 mm (modelo). A baixa correlação entre as médias das duas séries indica que o ETaHadGEM2-ES não é satisfatório para a região. O uso da base global CRU como referência, associada aos altos gradientes de altitude na região, pode ter limitado a performance do modelo. Recomenda-se análise das projeções com base em dados de estações locais para verificar se os resultados confirmam a baixa destreza do modelo para precipitação