Phylogenetic relationships of the Wolbachia of nematodes and arthropods

Wolbachia are well known as bacterial symbionts of arthropods, where they are reproductive parasites, but have also been described from nematode hosts, where the symbiotic interaction has features of mutualism. The majority of arthropod Wolbachia belong to clades A and B, while nematode Wolbachia mostly belong to clades C and D, but these relationships have been based on analysis of a small number of genes. To investigate the evolution and relationships of Wolbachia symbionts we have sequenced over 70 kb of the genome of wOvo, a Wolbachia from the human-parasitic nematode Onchocerca volvulus, and compared the genes identified to orthologues in other sequenced Wolbachia genomes. In comparisons of conserved local synteny, we find that wBm, from the nematode Brugia malayi, and wMel, from Drosophila melanogaster, are more similar to each other than either is to wOvo. Phylogenetic analysis of the protein-coding and ribosomal RNA genes on the sequenced fragments supports reciprocal monophyly of nematode and arthropod Wolbachia. The nematode Wolbachia did not arise from within the A clade of arthropod Wolbachia, and the root of the Wolbachia clade lies between the nematode and arthropod symbionts. Using the wOvo sequence, we identified a lateral transfer event whereby segments of the Wolbachia genome were inserted into the Onchocerca nuclear genome. This event predated the separation of the human parasite O. volvulus from its cattle-parasitic sister species, O. ochengi. The long association between filarial nematodes and Wolbachia symbionts may permit more frequent genetic exchange between their genomes

Obesity and the food environment: income and ethnicity differences among people with diabetes: the Diabetes Study of Northern California (DISTANCE).

ObjectiveIt is unknown whether any association between neighborhood food environment and obesity varies according to individual income and/or race/ethnicity. The objectives of this study were to test whether there was an association between food environments and obesity among adults with diabetes and whether this relationship differed according to individual income or race/ethnicity.Research design and methodsSubjects (n = 16,057) were participants in the Diabetes Study of Northern California survey. Kernel density estimation was used to create a food environment score for each individual's residence address that reflected the mix of healthful and unhealthful food vendors nearby. Logistic regression models estimated the association between the modeled food environment and obesity, controlling for confounders, and testing for interactions between food environment and race/ethnicity and income.ResultsThe authors found that more healthful food environments were associated with lower obesity in the highest income groups (incomes 301-600% and >600% of U.S. poverty line) among whites, Latinos, and Asians. The association was negative, but smaller and not statistically significant, among high-income blacks. On the contrary, a more healthful food environment was associated with higher obesity among participants in the lowest-income group (<100% poverty threshold), which was statistically significant for black participants in this income category.ConclusionsThese findings suggest that the availability of healthful food environments may have different health implications when financial resources are severely constrained

Defect Prognostics Models for Spall Growth in Railroad Bearing Rolling Elements

Prevention of railroad bearing failures, which may lead to catastrophic derailments, is a central safety concern. Early detection of railway component defects, specifically bearing spalls, will improve overall system reliability by allowing proactive maintenance cycles rather than costly reactive replacement of failing components. A bearing health monitoring system will provide timely detection of flaws. However, absent a well verified model for defect propagation, detection can only be used to trigger an immediate component replacement. The development of such a model requires that the spall growth process be mapped out by accumulating associated signals generated by various size spalls. The addition of this information to an integrated health monitoring system will minimize operation disruption and maintain maximum accident prevention standards enabling timely and economical replacements of failing components. An earlier study done by the authors focused on bearing outer ring (cup) raceway defects. The developed model predicts that any cup raceway surface defect (i.e. spall) once reaching a critical size (spall area) will grow according to a linear correlation with mileage. The work presented here investigates spall growth within the inner rings (cones) of railroad bearings as a function of mileage. The data for this study were acquired from defective bearings that were run under various load and speed conditions utilizing specialized railroad bearing dynamic test rigs owned by the University Transportation Center for Railway Safety (UTCRS) at the University of Texas Rio Grande Valley (UTRGV). The experimental process is based on a testing cycle that allows continuous growth of railroad bearing defects until one of two conditions are met; either the defect is allowed to grow to a size that does not jeopardize the safe operation of the test rig, or the change in area of the spall is less than 10% of its previous size prior to the start of testing. The initial spall size is randomly distributed as it depends on the originating defect depth, size, and location on the rolling raceway. Periodic removal and disassembly of the railroad bearings was carried out for inspection and defect size measurement along with detailed documentation. Spalls were measured using optical techniques coupled with digital image analysis, as well as, with a manual coordinate measuring instrument with the resulting field of points manipulated in MatLab™. Castings were made of spalls using low-melting, zero-shrinkage bismuth-based alloys, so that a permanent record of the spall geometry and its growth history can be retained. The main result of this study is a preliminary model for spall growth, which can be coupled with bearing condition monitoring tools that will allow economical and effective scheduling of proactive maintenance cycles that aim to mitigate derailments, and reduce unnecessary train stoppages and associated costly delays on busy railways

The state of multiple sclerosis: current insight into the patient/health care provider relationship, treatment challenges, and satisfaction

Esclerosi múltiple; Relació pacient-proveïdor d’atenció mèdica; Satisfacció amb el tractamentEsclerosis múltiple; Relación paciente-proveedor de atención médica; Satisfacción con el tratamientoMultiple sclerosis; Patient-health care provider relationship; Treatment satisfactionBackground: Managing multiple sclerosis (MS) treatment presents challenges for both patients and health care professionals. Effective communication between patients with MS and their neurologist is important for improving clinical outcomes and quality of life. Methods: A closed-ended online market research survey was used to assess the current state of MS care from the perspective of both patients with MS (≥18 years of age) and neurologists who treat MS from Europe and the US and to gain insight into perceptions of treatment expectations/goals, treatment decisions, treatment challenges, communication, and satisfaction with care, based on current clinical practice. Results: A total of 900 neurologists and 982 patients completed the survey, of whom 46% self-identified as having remitting-relapsing MS, 29% secondary progressive MS, and 11% primary progressive MS. Overall, patients felt satisfied with their disease-modifying therapy (DMT); satisfaction related to comfort in speaking with their neurologist and participation in their DMT decision-making process. Patients who self-identified as having relapsing-remitting MS were more likely to be very satisfied with their treatment. Top challenges identified by patients in managing their DMT were cost, side effects/tolerability of treatment, and uncertainty if treatment was working. Half of the patients reported skipping doses, but only 68% told their health care provider that they did so. Conclusion: Several important differences in perception were identified between patients and neurologists concerning treatment selection, satisfaction, expectations, goals, and comfort discussing symptoms, as well as treatment challenges and skipped doses. The study results emphasize that patient/neurologist communication and patient input into the treatment decision-making process likely influence patient satisfaction with treatment

Video Nasty: The Moral Apocalypse in Koji Suzuki’s Ring

Although overshadowed by its filmic adaptations (Hideo Nakata, 1998 and Gore Verbinski, 2002), Koji Suzuki’s novel Ring (1991) is at the heart of the international explosion of interest in Japanese horror. This article seeks to explore Suzuki’s overlooked text. Unlike the film versions, the novel is more explicitly focused on the line between self-preservation and self-sacrifice, critiquing the ease with which the former is privileged over the latter. In the novel then, the horror of Sadako’s curse raises questions about the terrors of moral obligation: the lead protagonist (Asakawa) projects the guilt he feels over his self-interested actions, envisaging them as an all-consuming apocalypse

Interventions for the management of malignant pleural effusions:An updated network meta-analysis

Talc slurry and poudrage are effective pleurodesis agents. IPCs have lower pleurodesis rates but comparable breathlessness control and reduced risk of repeat invasive procedures. It is essential that patients have access to a range of treatment strategies. https://bit.ly/38v30y