62 research outputs found
Effects of rmBMP-7 on Osteoblastic Cells Grown on a Nanostructured Titanium Surface
This study evaluates the effects of the availability of exogenous BMP-7 on osteoblastic cells’ differentiation on a nanotextured Ti surface obtained by chemical etching (Nano-Ti). The MC3T3-E1 and UMR-106 osteoblastic cell lines were cultured for 5 and 7 days, respectively, on a Nano-Ti surface and on a control surface (Control-Ti) in an osteogenic medium supplemented with either 40 or 200 ng/mL recombinant mouse (rm) BMP-7. The results showed that MC3T3-E1 cells exhibited distinct responsiveness when exposed to each of the two rmBMP-7 concentrations, irrespective of the surface. Even with 40 ng/mL rmBMP-7, important osteogenic effects were noticed for Control-Ti in terms of cell proliferation potential; Runx2, Osx, Alp, Bsp, Opn, and Smad1 mRNA expression; and in situ ALP activity. For Nano-Ti, the effects were limited to higher Alp, Bsp, and Opn mRNA expression and in situ ALP activity. On both surfaces, the osteogenic potential of UMR-106 cultures remained unaltered with 40 ng/mL rmBMP-7, but it was significantly reduced when the cultures were exposed to the 200 ng/mL concentration. The availability of rmBMP-7 to pre-osteoblastic cells at the concentrations used alters the expression profile of osteoblast markers, indicative of the acquisition of a more advanced stage of osteoblastic differentiation. This occurs less pronouncedly on the nanotextured Ti and without reflecting in higher mineralized matrix production by differentiated osteoblasts on both surfaces
Influence of a Physiologically Formed Blood Clot on Pre-Osteoblastic Cells Grown on a BMP-7-Coated Nanoporous Titanium Surface
Titanium (Ti) nanotopography modulates the osteogenic response to exogenous bone morphogenetic protein 7 (BMP-7) in vitro, supporting enhanced alkaline phosphatase mRNA expression and activity, as well as higher osteopontin (OPN) mRNA and protein levels. As the biological effects of OPN protein are modulated by its proteolytic cleavage by serum proteases, this in vitro study evaluated the effects on osteogenic cells in the presence of a physiological blood clot previously formed on a BMP-7-coated nanostructured Ti surface obtained by chemical etching (Nano-Ti). Pre-osteoblastic MC3T3-E1 cells were cultured during 5 days on recombinant mouse (rm) BMP-7-coated Nano-Ti after it was implanted in adult female C57BI/6 mouse dorsal dermal tissue for 18 h. Nano-Ti without blood clot or with blood clot at time 0 were used as the controls. The presence of blood clots tended to inhibit the expression of key osteoblast markers, except for Opn, and rmBMP-7 functionalization resulted in a tendency towards relatively greater osteoblastic differentiation, which was corroborated by runt-related transcription factor 2 (RUNX2) amounts. Undetectable levels of OPN and phosphorylated suppressor of mothers against decapentaplegic (SMAD) 1/5/9 were noted in these groups, and the cleaved form of OPN was only detected in the blood clot immediately prior to cell plating. In conclusion, the strategy to mimic in vitro the initial interfacial in vivo events by forming a blood clot on a Ti nanoporous surface resulted in the inhibition of pre-osteoblastic differentiation, which was minimally reverted with an rmBMP-7 coating
Epithelial maturation and molecular biology of oral HPV
Human papillomavirus (HPV) is widespread and can cause latent infection in basal cells, with low HPV DNA copy-number insufficient for transmission of infection; can cause subclinical infection that is active but without clinical signs; or can cause clinical infection leading to benign, potentially malignant or malignant lesions. The HPV cycle is influenced by the stage of maturation of the infected keratinocytes, and the production of virions is restricted to the post-mitotic suprabasal epithelial cells where all the virus genes are expressed
Porous Titanium Associated with CaP Coating: In Vivo and In Vitro Osteogenic Performance
Uma capitania dos novos tempos: economia, sociedade e política na São Paulo restaurada (1765-1822)
O artigo reflete sobre a trajetória da Capitania de São Paulo, a partir de 1750, apontando sua transformação, de fronteira e "boca do sertão", para território estratégico da conquista e defesa das partes meridionais e área economicamente integrada aos circuitos mercantis atlânticos.In this article, we reflect upon the history of the Captaincy of São Paulo as from 1750, drawing attention to its transformation from frontier land and "door to the backcountry" into a territory of strategic value for the purposes of conquest and defense of the southern regions, and economically integrated into the Atlantic trade routes
Densidade mineral óssea associada a características físicas e estilo de vida em adolescentes
Multiwavelength Observations of the Blazar VER J0521+211 during an Elevated TeV Gamma-Ray State
We report on a long-lasting, elevated gamma-ray flux state from VER J0521+211 observed by VERITAS, MAGIC, and Fermi-LAT in 2013 and 2014. The peak integral flux above 200 GeV measured with the nightly binned light curve is (8.8 ± 0.4) × 10-7 photons m-2 s-1, or ∼37% of the Crab Nebula flux. Multiwavelength observations from X-ray, UV, and optical instruments are also presented. A moderate correlation between the X-ray and TeV gamma-ray fluxes was observed, and the X-ray spectrum appeared harder when the flux was higher. Using the gamma-ray spectrum and four models of the extragalactic background light (EBL), a conservative 95% confidence upper limit on the redshift of the source was found to be z ≤ 0.31. Unlike the gamma-ray and X-ray bands, the optical flux did not increase significantly during the studied period compared to the archival low-state flux. The spectral variability from optical to X-ray bands suggests that the synchrotron peak of the spectral energy distribution (SED) may become broader during flaring states, which can be adequately described with a one-zone synchrotron self-Compton model varying the high-energy end of the underlying particle spectrum. The synchrotron peak frequency of the SED and the radio morphology of the jet from the MOJAVE program are consistent with the source being an intermediate-frequency-peaked BL Lac object
A lower bound on intergalactic magnetic fields from time variability of 1ES 0229+200 from MAGIC and Fermi/LAT observations
Extended and delayed emission around distant TeV sources induced by the
effects of propagation of gamma rays through the intergalactic medium can be
used for the measurement of the intergalactic magnetic field (IGMF). We search
for delayed GeV emission from the hard-spectrum TeV blazar 1ES 0229+200 with
the goal to detect or constrain the IGMF-dependent secondary flux generated
during the propagation of TeV gamma rays through the intergalactic medium. We
analyze the most recent MAGIC observations over a 5 year time span and
complement them with historic data of the H.E.S.S. and VERITAS telescopes along
with a 12-year long exposure of the Fermi/LAT telescope. We use them to trace
source evolution in the GeV-TeV band over one-and-a-half decade in time. We use
Monte Carlo simulations to predict the delayed secondary gamma-ray flux,
modulated by the source variability, as revealed by TeV-band observations. We
then compare these predictions for various assumed IGMF strengths to all
available measurements of the gamma-ray flux evolution. We find that the source
flux in the energy range above 200 GeV experiences variations around its
average on the 14 years time span of observations. No evidence for the flux
variability is found in 1-100 GeV energy range accessible to Fermi/LAT.
Non-detection of variability due to delayed emission from electromagnetic
cascade developing in the intergalactic medium imposes a lower bound of
B>1.8e-17 G for long correlation length IGMF and B>1e-14 G for an IGMF of the
cosmological origin. Though weaker than the one previously derived from the
analysis of Fermi/LAT data, this bound is more robust, being based on a
conservative intrinsic source spectrum estimate and accounting for the details
of source variability in the TeV energy band. We discuss implications of this
bound for cosmological magnetic fields which might explain the baryon asymmetry
of the Universe.Comment: 10 pages, 5 figures, accepted to A&A. Corresponding authors: Ievgen
Vovk, Paolo Da Vela (mailto:[email protected]) and Andrii Neronov
(mailto:[email protected]
Long-term multi-wavelength study of 1ES 0647+250
The BL Lac object 1ES 0647+250 is one of the few distant -ray
emitting blazars detected at very high energies (VHE, 100 GeV) during
a non-flaring state. It was detected with the MAGIC telescopes during its low
activity in the years 2009-2011, as well as during three flaring activities in
the years 2014, 2019 and 2020, with the highest VHE flux in the latter epoch.
An extensive multi-instrument data set was collected within several coordinated
observing campaigns throughout these years. We aim to characterise the
long-term multi-band flux variability of 1ES 0647+250, as well as its broadband
spectral energy distribution (SED) during four distinct activity states
selected in four different epochs, in order to constrain the physical
parameters of the blazar emission region under certain assumptions. We evaluate
the variability and correlation of the emission in the different energy bands
with the fractional variability and the Z-transformed Discrete Correlation
Function, as well as its spectral evolution in X-rays and rays. Owing
to the controversy in the redshift measurements of 1ES 0647+250 reported in the
literature, we also estimate its distance in an indirect manner through the
comparison of the GeV and TeV spectra from simultaneous observations with
Fermi-LAT and MAGIC during the strongest flaring activity detected to date.
Moreover, we interpret the SEDs from the four distinct activity states within
the framework of one-component and two-component leptonic models, proposing
specific scenarios that are able to reproduce the available multi-instrument
data.Comment: 20 pages, 7 figures. Accepted in A&A. Corresponding authors: Jorge
Otero-Santos; Daniel Morcuende; Vandad Fallah Ramazani; Daniela Dorner; David
Paneque (mailto: [email protected]
Multi-year characterisation of the broad-band emission from the intermittent extreme BL Lac 1ES~2344+514
The BL Lac 1ES 2344+514 is known for temporary extreme properties (e.g., a
shift of the synchrotron SED peak energy above 1keV). While
those extreme states were so far observed only during high flux levels,
additional multi-year observing campaigns are required to achieve a coherent
picture. Here, we report the longest investigation of the source from radio to
VHE performed so far, focusing on a systematic characterisation of the
intermittent extreme states. While our results confirm that 1ES 2344+514
typically exhibits 1keV during elevated flux periods, we also
find periods where the extreme state coincides with low flux activity. A strong
spectral variability thus happens in the quiescent state, and is likely caused
by an increase of the electron acceleration efficiency without a change in the
electron injection luminosity. We also report a strong X-ray flare (among the
brightest for 1ES 2344+514) without a significant shift of .
During this particular flare, the X-ray spectrum is among the softest of the
campaign. It unveils complexity in the spectral evolution, where the common
harder-when-brighter trend observed in BL Lacs is violated. During a low and
hard X-ray state, we find an excess of the UV flux with respect to an
extrapolation of the X-ray spectrum to lower energies. This UV excess implies
that at least two regions contribute significantly to the
infrared/optical/ultraviolet/X-ray emission. Using the simultaneous MAGIC,
XMM-Newton, NuSTAR, and AstroSat observations, we argue that a region possibly
associated with the 10 GHz radio core may explain such an excess. Finally, we
investigate a VHE flare, showing an absence of simultaneous variability in the
0.3-2keV band. Using a time-dependent leptonic modelling, we show that this
behaviour, in contradiction to single-zone scenarios, can instead be explained
by a two-component model.Comment: Accepted for publication in Astronomy & Astrophysic
- …