12,018 research outputs found
Shedding of NG2 by MMP-13 Attenuates Anoikis
Disruption of cell?matrix interactions can lead to anoikis?apoptosis due to loss of matrix contacts. We previously showed that Nerve/glial antigen 2 (NG2) is a novel anoikis receptor. Specifically, overexpression of NG2 leads to anoikis propagation, whereas its suppression leads to anoikis attenuation. Interestingly, NG2 expression decreases in late anoikis, suggesting that NG2 reduction is also critical to this process. Thus, we hypothesized that NG2 undergoes cleavage to curtail anoikis propagation. Further, since matrix metalloproteinases (MMPs) cleave cell surface receptors, play a major role in modulating apoptosis, and are associated with death receptor cleavage during apoptosis, we further hypothesized that cleavage of NG2 could be mediated by MMPs to regulate anoikis. Indeed, anoikis conditions triggered release of the NG2 extracellular domain into condition media during late apoptosis, and this coincided with increased MMP-13 expression. Treatment with an MMP-13 inhibitor and MMP-13 siRNA increased anoikis, since these treatments blocked NG2 release. Further, NG2-positive cells exhibited increased anoikis upon MMP-13 inhibition, whereas MMP-13 inhibition did not increase anoikis in NG2-null cells, corroborating that retention of NG2 on the cell membrane is critical for sustaining anoikis, and its cleavage for mediating anoikis attenuation. Similarly, NG2 suppression with siRNA inhibited NG2 release and anoikis. In contrast, MMP-13 overexpression or exogenous MMP-13 reduced anoikis by more effectively shedding NG2. In conclusion, maintenance of NG2 on the cell surface promotes anoikis propagation, whereas its shedding by MMP-13 actions attenuates anoikis. Given that these findings are derived in the context of periodontal ligament fibroblasts, these data have implications for periodontal inflammation and periodontal disease pathogenesis.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140362/1/dna.2014.2399.pd
A comparison of the different anisometropic testing procedures as done at Pacific University
A comparison of the different anisometropic testing procedures as done at Pacific Universit
Detection-Loophole-Free Test of Quantum Nonlocality, and Applications
We present a source of entangled photons that violates a Bell inequality free
of the "fair-sampling" assumption, by over 7 standard deviations. This
violation is the first experiment with photons to close the detection loophole,
and we demonstrate enough "efficiency" overhead to eventually perform a fully
loophole-free test of local realism. The entanglement quality is verified by
maximally violating additional Bell tests, testing the upper limit of quantum
correlations. Finally, we use the source to generate secure private quantum
random numbers at rates over 4 orders of magnitude beyond previous experiments.Comment: Main text: 5 pages, 2 figures, 1 table. Supplementary Information: 7
pages, 2 figure
First Astronomical Application of a Cryogenic TES Spectrophotometer
We report on the first astronomical observations with a photon counting pixel
detector that provides arrival time- (delta t = 100ns) and energy- (delta
E_gamma < 0.15eV) resolved measurements from the near IR through the near UV.
Our test observations were performed by coupling this Transition Edge Sensor
(TES) device to a 0.6m telescope; we have obtained the first simultaneous
optical near-IR phase-resolved spectra of the Crab pulsar. A varying infrared
turnover gives evidence of self-absorption in the pulsar plasma. The potential
of such detectors in imaging arrays from a space platform are briefly
described.Comment: 4 pages, 5 figure
Kinematic modelling of a 3-axis NC machine tool in linear and circular interpolation
Machining time is a major performance criterion when it comes to high-speed
machining. CAM software can help in estimating that time for a given strategy.
But in practice, CAM-programmed feed rates are rarely achieved, especially
where complex surface finishing is concerned. This means that machining time
forecasts are often more than one step removed from reality. The reason behind
this is that CAM routines do not take either the dynamic performances of the
machines or their specific machining tolerances into account. The present
article seeks to improve simulation of high-speed NC machine dynamic behaviour
and machining time prediction, offering two models. The first contributes
through enhanced simulation of three-axis paths in linear and circular
interpolation, taking high-speed machine accelerations and jerks into account.
The second model allows transition passages between blocks to be integrated in
the simulation by adding in a polynomial transition path that caters for the
true machining environment tolerances. Models are based on respect for path
monitoring. Experimental validation shows the contribution of polynomial
modelling of the transition passage due to the absence of a leap in
acceleration. Simulation error on the machining time prediction remains below
1%
The Cosmic Infrared Background Experiment (CIBER): Instrumentation and First Results
Ultraviolet emission from the first generation of stars in the Universe ionized the intergalactic medium in a process which was completed by z similar to 6; the wavelength of these photons has been redshifted by (1 + z) into the near infrared today and can be measured using instruments situated above the Earth's atmosphere. First flying in February 2009, the Cosmic Infrared Background ExpeRiment (CIBER) comprises four instruments housed in a single reusable sounding rocket borne payload. CIBER will measure spatial anisotropies in the extragalactic IR background caused by cosmological structure from the epoch of reionization using two broadband imaging instruments, make a detailed characterization of the spectral shape of the IR background using a low resolution spectrometer, and measure the absolute brightness of the Zodiacal light foreground with a high resolution spectrometer in each of our six science fields. The scientific motivation for CIBER and details of its first and second flight instrumentation will be discussed. First flight results on the color of the zodiacal light around 1 mu m and plans for the future will also be presented
Effect of anisotropy on universal transport in unconventional superconductors
We investigate the universal electronic transport for a mixed
+s-wave superconductor in the presence of an anisotropic
elliptical Fermi surface. Similar to the universal low-temperature transport
predicted in a -wave superconductor with a circular Fermi surface,
anisotropic universal features are found in the low-temperature microwave
conductivity, and thermal conductivity in the anisotropic system. The effects
of anisotropy on the penetration depth, impurity induced suppression, and
the zero-frequency density of states are also considered. While a small amount
of anisotropy can lead to a strong suppression of the effective scattering rate
and hence the density of states at zero frequency, experimental data suggests
that large effects are restored by a negative -component gap admixture.Comment: 8 page
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