Large-scale T-DNA mutagenesis in Arabidopsis for functional genomic analysis

In planta Agrobacterium-mediated transformation combined with a soil-based herbicide selection for transgenic plants was used to recover large numbers of transgenic Arabidopsis plants for functional genomic studies. A tissue-culture-free system for generating transgenic plants was achieved by infiltrating Arabidopsis plants with Agrobacterium tumefaciens harboring a binary T-DNA vector containing the phosphinothricin acetyltransferase gene from Streptomyces hygroscopicus, and by selecting transgenic Arabidopsis growing in soil by foliar application of the herbicide Finale (phosphinothricin). Analysis of herbicide-resistant plants indicated that all were transgenic and that the T-DNA transformation process occurred late during flower development, resulting in a preponderance of independently derived T-DNA insertions. T-DNA insertions were usually integrated in a concatenated, rearranged form, and using linkage analysis, we estimated that T1 plants carried between one and five T-DNA loci. Using pooling strategies, both DNA and seed pools were generated from about 38,000 Arabidopsis plants representing over 115,000 independent T-DNA insertions. We show the utility of these transgenic lines for identifying insertion mutations using gene sequence and PCR-based screening

Improving Debriefing and Exclusion Practices in Psychological Research

Project aimed to improve debriefing and exclusion practices in psychological studies using deceptio

The Bi-Dimensional Rejection Taxonomy: Organizing Responses to Social Rejection along Antisocial–Prosocial and Engaged–Disengaged Dimensions

Interpersonal responses to social rejection vary widely in form and function. Existing theories of social rejection have exclusively focused on organizing these responses on a single antisocial–prosocial dimension. Accumulating evidence suggests a gap in this approach: variability in social responses to rejection cannot solely be explained by the antisocial–prosocial dimension alone. To fill this gap, we propose the bi-dimensional rejection taxonomy, consisting of the antisocial–prosocial x-axis and engaged-disengaged y-axis, a novel contribution to the literature. We demonstrate that both the x- and y-axes are necessary for understanding interpersonal responses to rejection and avoiding erroneous conclusions. We also show how this new framework allows researchers to generate more nuanced and accurate hypotheses about how people respond when rejected. We further demonstrate how existing research about individual differences and situational factors that predict responses to rejection can be viewed in a new light within the bi-dimensional rejection taxonomy. We conclude by suggesting how the taxonomy inspires innovative questions for future research, including understanding spontaneous responses and neurophysiological markers

MASTER - Close Relationships and Health Lab

This is the parent project for all studies conducted in the Close Relationships and Health Lab (PI: Lisa Jaremka

Supplemental Material, SPPS762301_suppl_mat - Does the Prospect of Fulfilling Belonging Affect Social Responses to Rejection? A Conceptual Replication Attempt

<p>Supplemental Material, SPPS762301_suppl_mat for Does the Prospect of Fulfilling Belonging Affect Social Responses to Rejection? A Conceptual Replication Attempt by Naoyuki Sunami, Megan A. Nadzan, Lisa M. Jaremka in Social Psychological and Personality Science</p