Triterpenoids from vitellaria paradoxa stem barks reduce nitrite levels in lps-stimulated macrophages

open7siVitellaria paradoxa C. F. Gaertn is widely used in African traditional medicine as an anti-inflammatory remedy to treat rheumatism, gastric problems, diarrhea, and dysentery. The phyto-chemical investigation of the ethyl acetate extract of V. paradoxa stem bark collected in Burkina Faso led to the isolation of eight known and two triterpenes undescribed to date (7 and 10), in the free alcohol form or as acetyl and cinnamyl ester derivatives. The stereostructures of the new compounds were elucidated using HR-ESIMS and 1D and 2D NMR data. The isolated compounds were evaluated in vitro for their inhibitory effect on nitrite levels on murine macrophages J774 stimulated with the lipopolysaccharide (LPS). Among all the compounds tested, lupeol cinnamate (3) and betulinic acid (5) showed a beneficial effect in reducing nitrite levels produced after LPS stimulation.openSirignano C.; Nadembega P.; Poli F.; Romano B.; Lucariello G.; Rigano D.; Taglialatela-Scafati O.Sirignano C.; Nadembega P.; Poli F.; Romano B.; Lucariello G.; Rigano D.; Taglialatela-Scafati O

World Viral Hepatitis Day in Burkina Faso, 2020: awareness, prevention and hepatitis B vaccine to achieve the WHO eradication goal by 2030

Running title: Hepatitis B vaccine coverage in Ouagadougou, Burkina Faso Burkina Faso is located in a region where hepatitis B is endemic with low vaccination coverage. The aim of this study was to evaluate the vaccine coverage during Hepatitis B screening campaign organized by "SOS Hepatitis Burkina"in Ouagadougou in July 2020. A rapid HBsAg detection test (Abon Biopharma Guangzhou, Co., Ltd. China; sensitivity > 99.0%; specificity, 96.8%) was performed on 252 individuals who voluntarily agreed to participate in the study. This study was approved by the Institutional Ethics Committee of the Centre de recherche Biomoleculaire Pietro Annigoni (CERBA).We included 252 participants aged 2 to 77 years (mean age 31.77±14.07 and 29.67±13.71 for males and females, respectively). The prevalence of HBsAg was 18.25% (46/252) and was slightly high in the age groups 21-30 years (23.71%) and 31-40 years (23.73%). Out of 252 participants, 25 HBV-negative participants were vaccinated, giving a vaccination rate of 9.92%.This study reports a high prevalence of HBV infection in Burkina Faso with low vaccination coverage. The study suggests that more attention should be paid to HBV prevention in Burkina Fas