27 research outputs found
Association of Helicobacter pylori restriction endonuclease-replacing gene, hrgA with overt gastrointestinal diseases
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Polymerase chain reaction based analysis of the cytotoxin associated gene pathogenicity island of Helicobacter pylori from saliva: An approach for rapid molecular genotyping in relation to disease status
No full textOral Health Status and Oral Hygiene Practices of Patients with Peptic Ulcer and How These Affect Helicobacter pylori Eradication from the Stomach
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Helicobacter pylori in a Costa Rican dyspeptic patient population
No full textArtÃculo cientÃfico -- Universidad de Costa Rica, Instituto de Investigaciones en Salud. 1994. Este documento es privado debido a limitaciones de derechos de autor.Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination.Helicobacter pylori DNA was detected by PCR in 60 (92 %) of the patients, andHelicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98 % (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material.Universidad de Costa Rica. Instituto de Investigaciones en SaludUCR::VicerrectorÃa de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones en Salud (INISA
