Fat content reduction and lipid profile improvement in Portuguese fermented sausages alheira

Due to the current trend to reduce fat consumption, the meat food industry, has been increasing the strategies to produce and commercialize products where the reduction or even elimination of saturated fat is an important goal. This study aimed to test different formulas to reduce the fat content and improve the lipid profile in the Portuguese fermented sausage alheira. Data indicate that the three composition formulas of alheiras tested are not a hypercaloric product and the fatty acid profile could be improved changing the sources of fat and specie of meat, particularly reducing the palmitic and stearic acids and the myristic acid contents and increasing the oleic, linoleic and palmitoleic acid contents. The industry could diversify its offer with two new composition formulas, a pork-free alheira, and oil-free alheira, reducing the fat content and improving the fatty acid profile.The authors are grateful to Bísaro – Salsicharia Tradicional industry and to Laboratory of Carcass and Meat Quality of Agriculture School of Polytechnic Institute of Bragança ‘Cantinho do Alfredo’. The authors are members of the Healthy Meat network, funded by CYTED (ref. 119RT0568).info:eu-repo/semantics/publishedVersio

RNA polymerase II stalling promotes nucleosome occlusion and pTEFb recruitment to drive immortalization by Epstein-Barr virus

Epstein-Barr virus (EBV) immortalizes resting B-cells and is a key etiologic agent in the development of numerous cancers. The essential EBV-encoded protein EBNA 2 activates the viral C promoter (Cp) producing a message of ~120 kb that is differentially spliced to encode all EBNAs required for immortalization. We have previously shown that EBNA 2-activated transcription is dependent on the activity of the RNA polymerase II (pol II) C-terminal domain (CTD) kinase pTEFb (CDK9/cyclin T1). We now demonstrate that Cp, in contrast to two shorter EBNA 2-activated viral genes (LMP 1 and 2A), displays high levels of promoter-proximally stalled pol II despite being constitutively active. Consistent with pol II stalling, we detect considerable pausing complex (NELF/DSIF) association with Cp. Significantly, we observe substantial Cp-specific pTEFb recruitment that stimulates high-level pol II CTD serine 2 phosphorylation at distal regions (up to +75 kb), promoting elongation. We reveal that Cp-specific pol II accumulation is directed by DNA sequences unfavourable for nucleosome assembly that increase TBP access and pol II recruitment. Stalled pol II then maintains Cp nucleosome depletion. Our data indicate that pTEFb is recruited to Cp by the bromodomain protein Brd4, with polymerase stalling facilitating stable association of pTEFb. The Brd4 inhibitor JQ1 and the pTEFb inhibitors DRB and Flavopiridol significantly reduce Cp, but not LMP1 transcript production indicating that Brd4 and pTEFb are required for Cp transcription. Taken together our data indicate that pol II stalling at Cp promotes transcription of essential immortalizing genes during EBV infection by (i) preventing promoter-proximal nucleosome assembly and ii) necessitating the recruitment of pTEFb thereby maintaining serine 2 CTD phosphorylation at distal regions

Experimental investigation on the bond behavior of a compatible TRM-based solution for rammed earth heritage

Despite the current awareness of the high seismic risk of earthen structures, little has been done so far to develop proper strengthening solutions for the rammed earth heritage. Based on the effectiveness of TRM for masonry buildings, the strengthening of rammed earth walls with externally bonded fibers using earth-based mortar is being proposed as a compatible solution. In this context, the investigation of bond behavior was conducted by means of direct tensile tests, pull-out tests and single lap-shear tests. The specimens were prepared using earth-based mortars and two different types of meshes (glass and nylon) while considering different-bonded lengths. The direct tensile tests on TRM coupons showed the high capacity of the nylon mesh in transferring stresses after cracking of the mortar. The pull-out tests highlighted that in the case of glass fiber mesh, the bond was granted by friction, while the mechanical anchorage promoted by the transversal yarns granted the bond of the nylon mesh. Finally, the single lap-shear tests showed that the adopted earth-based mortar seems to limit the performance of the strengthening.This work was supported by the Fundacao para a Ciencia e a Tecnologia [PTDC/ECM-EST/2777/2014, SFRH/BD/131006/2017, SFRH/BPD/97082/2013]