10 research outputs found
Multi-Modality Analysis Improves Survival Prediction in Enucleated Uveal Melanoma Patients
PURPOSE. Uveal melanoma (UM) is characterized by multiple chromosomal rearrangements
and recurrent mutated genes. The aim of this study was to investigate if copy number
variations (CNV) alone and in combination with other genetic and clinico-histopathological
variables can be used to stratify for disease-free survival (DFS) in enucleated patients with UM.
METHODS. We analyzed single nucleotide polymorphisms (SNP) array data of primary tumors
and other clinical variables of 214 UM patients from the Rotterdam Ocular Melanoma Study
(ROMS) cohort. Nonweighted hierarchical clustering of SNP array data was used to identify
molecular subclasses with distinct CNV patterns. The subclasses associate with mutational
status of BAP1, SF3B1, or EIF1AX. Cox proportional hazard models were then used to study
the predictive performance of SNP array cluster-, mutation-, and clinico-histopathological
data, and their combination for study endpoint risk.
RESULTS. Five clusters with distinct CNV patterns and concomitant mutations in BAP1, SF3B1,
or EIF1AX were identified. The sample’s cluster allocation contributed significantly to
mutational status of samples in predicting the incidence of metastasis during a median of 45.6
(interquartile range [IQR]: 24.7–81.8) months of follow-up (P < 0.05) and vice versa.
Furthermore, incorporating all data sources in one model yielded a 0.797 C-score during 100
months of follow-up.
CONCLUSIONS. UM has distinct CNV patterns that correspond to different mutated driver genes.
Incorporating clinico-histopathological, cluster and mutation data in the analysis results in
good performance for UM-related DFS prediction
Expanding the living donor pool using domino liver transplantation: a systematic review
Introduction: To this day, a discrepancy exists between donor liver demand and supply. Domino liver transplantation (DLT) can contribute to increasing the number of donor livers available for transplantation. Methods: The design of this systematic review was based on the Preferred Reporting Items for Systematic Reviews (PRISMA). A qualitative analysis of included studies was performed. Primary outcomes were mortality and peri- and postoperative complications related to DLT. Results: Twelve studies met the inclusion criteria. All included studies showed that DLT outcomes were comparable to outcomes of deceased donor liver transplantation (DDLT) in terms of mortality and complications. One-year patient survival rate ranged from 66.7% to 100%. Re-transplantation rate varied from 0 to 12.5%. Most frequent complications were related to biliary (3.7%–37.5%), hepatic artery (1.6%–9.1%), portal vein (12.5–33.3%) and hepatic vein events (1.6%), recurrence of domino donor disease (3.3%–17.4%) and graft rejection (16.7%–37.7%). The quality of the evidence was rated as moderate according to the Newcastle–Ottawa scale (NOS). Conclusion: DLT outcomes were similar to DDLT in terms of mortality and complications. Even though DLT will not solve the entire problem of organ shortage, transplant programs should always consider using this tool to maximize the availability of liver grafts
Application of Azure C for the extractive spectrophotometric determination of microgram amounts of penicillin
Multi-Modality Analysis Improves Survival Prediction in Enucleated Uveal Melanoma Patients
textabstractPURPOSE. Uveal melanoma (UM) is characterized by multiple chromosomal rearrangements
and recurrent mutated genes. The aim of this study was to investigate if copy number
variations (CNV) alone and in combination with other genetic and clinico-histopathological
variables can be used to stratify for disease-free survival (DFS) in enucleated patients with UM.
METHODS. We analyzed single nucleotide polymorphisms (SNP) array data of primary tumors
and other clinical variables of 214 UM patients from the Rotterdam Ocular Melanoma Study
(ROMS) cohort. Nonweighted hierarchical clustering of SNP array data was used to identify
molecular subclasses with distinct CNV patterns. The subclasses associate with mutational
status of BAP1, SF3B1, or EIF1AX. Cox proportional hazard models were then used to study
the predictive performance of SNP array cluster-, mutation-, and clinico-histopathological
data, and their combination for study endpoint risk.
RESULTS. Five clusters with distinct CNV patterns and concomitant mutations in BAP1, SF3B1,
or EIF1AX were identified. The sample’s cluster allocation contributed significantly to
mutational status of samples in predicting the incidence of metastasis during a median of 45.6
(interquartile range [IQR]: 24.7–81.8) months of follow-up (P < 0.05) and vice versa.
Furthermore, incorporating all data sources in one model yielded a 0.797 C-score during 100
months of follow-up.
CONCLUSIONS. UM has distinct CNV patterns that correspond to different mutated driver genes.
Incorporating clinico-histopathological, cluster and mutation data in the analysis results in
good performance for UM-related DFS prediction