16 research outputs found
Translational in vitro activity of the 3a gene and the coat protein gene derived from brome mosaic virus RNA 3 by site-specific cleavage with RNase H
AbstractTwo translationally active fragments were derived from the dicistronic Brome Mosaic Virus (BMV) RNA 3 by site-specific cleavage with RNase H from E.coli: the 5β²-proximal (L)fragment encoding the 32 kDa protein and the 3β²-proximal (Sh) fragment carrying the coat protein gene. The translational efficiency of the L- and Sh-fragments was compared with those of the native BMV RNA 3 and RNA 4, encoding the 32 kDa and coat proteins, respectively. The Sh-fragment template activity was similar to that of RNA 4, although it was uncapped and contained 20β22 additional 5β²-terminal nucleotides in comparison with BMV RNA 4
ΠΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ² ΠΏΠ»Π°Π·ΠΌΡ ΠΊΡΠΎΠ²ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π²Π½ΡΡΡΠ΅Π½Π½ΠΈΡ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ ΡΡΠ°Π½Π΄Π°ΡΡΠΎΠ² Π² ΠΏΠ°Π½ΠΎΡΠ°ΠΌΠ½ΠΎΠΌ ΠΏΡΠΎΡΠ΅ΠΎΠΌΠ½ΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π΅
LC-MS/MS allows identification of thousands of proteins in the complex proteomes. However, a significant part of a proteome remains inaccessible for identification due to the absence or poor quality of MS/MS spectra. The method described herein allows identifying the desired proteins of human blood plasma by comparing aligned chromatographic data of digested by trypsin sample and the same sample with spikedin synthetic peptides. Identification of human blood plasma proteins is archived by assigning tandem mass spectra of spiked-in peptides to the corresponding aligned chromatographic peaks of proteolytic peptides. Using the described approach we have identified 19 low abundant proteins in human blood plasma, which corresponded to 19 synthetic peptides used in the study. SRM verification of the identifications with isotopically labelled standards (SIS) confirmed the presence in the plasma of above 17 proteins.ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΏΠ°Π½ΠΎΡΠ°ΠΌΠ½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ-ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ (ΠΠ₯-ΠΠ‘/ΠΠ‘) ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ Π² ΡΠ»ΠΎΠΆΠ½ΡΡ
ΠΏΡΠΎΡΠ΅ΠΎΠΌΠ°Ρ
Π΄ΠΎ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΈΡ
ΡΡΡΡΡ Π±Π΅Π»ΠΊΠΎΠ². ΠΠ΄Π½Π°ΠΊΠΎ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½Π°Ρ ΡΠ°ΡΡΡ ΠΏΡΠΎΡΠ΅ΠΎΠΌΠ° ΠΎΡΡΠ°Π΅ΡΡΡ Π½Π΅Π΄ΠΎΡΡΡΠΏΠ½ΠΎΠΉ Π΄Π»Ρ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΈΠ·-Π·Π° ΠΎΡΡΡΡΡΡΠ²ΠΈΡ ΠΈΠ»ΠΈ ΠΏΠ»ΠΎΡ
ΠΎΠ³ΠΎ ΠΊΠ°ΡΠ΅ΡΡΠ²Π° ΠΠ‘/ΠΠ‘ ΡΠΏΠ΅ΠΊΡΡΠΎΠ². ΠΠΏΠΈΡΡΠ²Π°Π΅ΠΌΡΠΉ Π² Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ ΠΏΠΎΠ²ΡΡΠ°Π΅Ρ Π²Π΅ΡΠΎΡΡΠ½ΠΎΡΡΡ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ² ΠΏΠ»Π°Π·ΠΌΡ ΠΊΡΠΎΠ²ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΏΡΡΠ΅ΠΌ Π²ΡΡΠ°Π²Π½ΠΈΠ²Π°Π½ΠΈΡ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π΄Π°Π½Π½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠ° ΡΠΌΠ΅ΡΠΈ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ² ΠΈ ΡΡΠΎΠ³ΠΎ ΠΆΠ΅ ΠΎΠ±ΡΠ°Π·ΡΠ°, ΡΠ°Π·Π±Π°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΏΠ΅ΠΏΡΠΈΠ΄Π°ΠΌΠΈ. Π’Π°ΠΊΠ°Ρ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΡ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΡΠΎΠΏΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΡ ΡΠ°Π½Π΄Π΅ΠΌΠ½ΡΡ
ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠ² ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ² Ρ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΠΌΠΈ Π²ΡΡΠΎΠ²Π½Π΅Π½Π½ΡΠΌΠΈ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΏΠΈΠΊΠ°ΠΌΠΈ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠ΅ΠΏΡΠΈΠ΄ΠΎΠ². ΠΡΠΏΠΎΠ»ΡΠ·ΡΡ Π΄Π°Π½Π½ΡΠΉ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄, ΠΌΡ Π²ΡΡΠ²ΠΈΠ»ΠΈ 19 Π½ΠΈΠ·ΠΊΠΎ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½ΡΡ
Π±Π΅Π»ΠΊΠΎΠ² ΠΏΠ»Π°Π·ΠΌΡ ΠΊΡΠΎΠ²ΠΈ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΡ
19 ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΏΠ΅ΠΏΡΠΈΠ΄Π°ΠΌ, Π²ΡΠ±ΡΠ°Π½Π½ΡΠΌ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΡΠΎΠ²Π΅ΡΠΊΠ° ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΌΠΎΠ½ΠΈΡΠΎΡΠΈΠ½Π³Π° Π΄ΠΈΡΡΠΎΡΠΈΠ°ΡΠΈΠ²Π½ΡΡ
ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄ΠΎΠ² Ρ ΠΈΠ·ΠΎΡΠΎΠΏΠ½ΠΎ-ΠΌΠ΅ΡΠ΅Π½ΡΠΌΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠ°ΠΌΠΈ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠ΄ΠΈΠ»Π° Π½Π°Π»ΠΈΡΠΈΠ΅ Π² ΠΏΠ»Π°Π·ΠΌΠ΅ 17 Π±Π΅Π»ΠΊΠΎΠ²
Translation enhancing properties of the 5β²-leader of potato virus X genomic RNA
AbstractThe double-stranded DNA copy corresponding to the 5β²-nontranslated Ξ±Ξ²-leader of potato virus X (PVX) genomic RNA (positions β3 to β85 according to AUG initiator) was chemically synthesized and fused to the transcription plasmids containing three different reporter genes: neomycinphosphotransferase type II (NPT II) gene, Bacillus thuringiensis coleopteran-specific toxic protein gene and Ξ²-glucuronidase (GUS) gene. Expression of the reporter genes in vitro and in plant protoplasts (in the case of GUS gene) reveals that the Ξ±Ξ²-leader of PVX RNA acts as a translation enhancer despite the presence of the upstream vector-derived sequence and irrespective of the length of the spacer sequence preceding the reporter genes
Manufacture and study of new polystyrene scintillators
The main optical characteristics and radiation hardness of new polystyrene scintillator UPS98GC were studied. The scintillator UPS-98GC was compared to SCSN-81, produced by Kuraray Co. which is often used in high-energy physics experiments. The dependence of scintillator properties on radiation dose rates as well as on total dose values was studied. It is shown that for relatively small dose rates closed to those expected during scintillator lifetime, our UPS98GC does not yield to SCSN-81