Topos “Karjala” in Bilingual Poetry by A. Volkov

The observations on the specifics of the Karjala topos in the works of the bilingual poet A. Volkov is for the first time presented in the article. It has been proved that in his mind “Karjala” is associated with “native land”, the memory of ancestors, native language, Livvik’s happiness itself. In Russian and Karelian-language poems, the recreated spaces of “Karjala” are semantically similar. This locus is open (the center of the universe) and closed  (the outskirts of Russia) at the same time. Like an archeologist, the author turns to the understanding of the mythological genealogy of “Karjala”, its ancestral homeland, proto-language, archetypes of national consciousness, totem signs. The authors found that the bilingual view of the universe allowed the poet to conceptualize in a new  way and reveal, in the context of world history and culture, the main concepts in the life of Livvik Karelians: native dialect, memory of tradition, Karelian genotype, Livvik happiness. Particular attention is paid to the analysis of the  socalled semantic “transitions” from one ethnic picture of the world to another (remembering is transformed into crying, the genesis of the native language as a gift from God — into the path to God). In the poetic reconstruction of the Karjala topos by A. Volkov, the Karelian worldview is fundamental, the Russian language and literature is a guide in the formation of an original poetic tradition. For the first time, the original poems of A. Volkov and their auto-translations were introduced into scientific circulation

Diagnosis of Neonatal Alloimmune Thrombocytopenia: Determination of the Specificity of Antiplatelet Alloantibodies in the Maternal Blood Plasma Using a Molecular and Genetic Method

Background: Thrombocytopenia occurs in 1-5 % of newborns (platelet count < 150 × 109/L). Low platelet count of 50 × 109/L leads to the hemorrhagic syndrome, with one of its causes being neonatal alloimmune thrombocytopenia resulting from incompatibility between the mother and the fetus with human platelet antigens (HPA) inherited from the father and absent in the mother, which leads to the formation of maternal antibodies. Anti-HPA-1a, anti-HPA-5b, anti-HPA-3a, and anti-HPA-3b antibodies are clinically significant as they destroy fetal/neonatal platelets causing severe complications (intracranial hemorrhage in 20 % of cases and prenatal or postnatal death in 10 % of cases). Adequate diagnosis is a key to a successful treatment approach, which largely depends on the thrombocytopenia cause.   Objective: To determine the alloimmune nature of neonatal thrombocytopenia and the specificity of antibodies in the mother’s blood.   Materials and methods: We studied blood samples of parents (21 pairs) of newborns with thrombocytopenia in Saint Petersburg, Russian Federation. We used flow cytometry to determine alloantibodies in the maternal plasma after incubation with paternal platelets and staining with Goat F(ab’)2 Anti-Human IgG-FITC and CD41-PE monoclonal antibodies. Allosensitization index was calculated as the percentage of IgG-positive cells to the number of cells fixing anti-CD41 antibodies. At the value of ≥ 15 %, antiplatelet alloantibodies were considered present in a sample. We used a molecular detection system of the FluoVista analyzer (Inno-Train, Germany) for genetic testing with allele-specific primers. Alleles of genes encoding the expression of HPA-1, HPA-2, HPA-3, HPA-4, HPA-5, HPA-6, HPA-9, and HPA-15 antigens were determined by a real-time polymerase chain reaction using a set of HPA-FluoGene reagents (Inno-Train, Germany). Genomic DNA was isolated using the DNA-sorb-B set (AmpliSens, Russian Federation).   Results: We found that 8 of 21 (38 %) mothers had antibodies against paternal platelets. During genotyping in mother/father pairs, incompatible combinations of platelet antigens were revealed: HPA-1b/HPA-1a in 9 pairs (HPA-1a antigen absent on the maternal platelets and present on the paternal platelets), of which 5 mothers (55%) had antibodies with a probable specificity to anti-HPA-1a; HPA-1a/HPA-1b incompatibility in 4 pairs, with 2 (50 %) mothers having antibodies with an anti-HPA-1b specificity. HPA-3a/HPA-3b incompatibility was observed in 4 pairs, with antibodies (probably anti-HPA3b) in 1 mother (25%). HPA-2a/HPA-2b, HPA-5a/HPA-5b, HPA-15a/HPA-15b, HPA-15b/HPA-15a incompatibilities were detected (1 case each in 21 pairs), with no antibodies found in mothers. The probable specificity of the antibodies was distributed as follows: 62 % for anti-HPA-1a, 25% for anti-HPA-1b, and 13 % for anti-HPA-3a.   Conclusions: We confirmed the immune nature of neonatal thrombocytopenia and determined the probable specificity of maternal alloantibodies in 8 of 21 cases

Features of interpretation of the results of studies of ABО and Rhesus antigens and antibodies in patients with hematological diseases

Aim. To assess the aspects of interpretation of pre-transfusion tests in patients with hematological diseases. Material and Methods. We performed an analysis of the results of serological studies of ABО, Rh blood groups in blood samples of 857 patients with oncohematological diseases. ABO blood group determination and typing of D, C, c, E, e, К antigens were carried out using a gel agglutination test. Results. The decrease in strength of the agglutination of standard red blood cells by the patient’s anti-A and/or anti-B antibodies was observed in 112 patients (13.07% of the total number of patients). Abnormal agglutination strength in ABO and Rh antigens testing was observed in 17 patients (1.98% of the total number of patients), among them were 7 patients with acute myeloid leukemia (AML), 6 - with сhronic myeloid leukemia (CML), 2 – myelodysplastic syndrome (MDS), 2 – polycythemia vera (PV). Double populations of red blood cells were mainly detected in patients with MDS (45.61 %), aplastic anemia (AA) (27.27 %), primary myelofibrosis (PMF) (22.73 %), acute leukemia (AL) (22.2 %). In most cases double populations were associated with previous transfusions of blood products, meanwhile, three patients from this group (two patients with CML and one patient with PV) had never received blood transfusions before. Conclusion. Differences in anti-A and anti-B antibodies content were much more common in patients with lymphoproliferative disorders (LPDs) than in patients with myeloproliferative neoplasms (MPNs) (85.71% and 8.04%, respectively), while decrease of expression of red blood cell antigens was more typical for MPNs and did not occur in patients with LPDs

Improve business process efficiency by value engineering

This article is devoted to the problems of business processes optimization by value engineering (FCA) method. The business processes analysis is particularly relevant in modern conditions. The purpose the investigation is to adapt the value engineering to the business process. The FCA is a universal and highly efficient method of parameters optimization and other structural, technological, organizational, economic characteristics of a product, work or services. The hypothesis of the applying a value engineering to a business process possibility is considered. The article discusses FCA tools as an example of a metal rolling business delivery process. A business process model is being constructed as an object structural element model. The functions decomposition is carried out. Functions are classified into basic and auxiliary on the basis of the level. In the article the significance and functional costs are determined. A functional-cost diagram is constructed to identify the functions with the most deviations needing improvement. The FCA stages are accompanied by graphical illustrations, tables that illustrate the logic of applying the method to the business process. As a result, an optimal business-process concept with the lowest cost is being developed. © Published under licence by IOP Publishing Ltd

Strusture, biomass and production of the biotic component of the ecosystem of an growing eutrophic reservoir

Using our own data and data from the literature, we assessed the total biomass of the biotic component of the ecosystem of the Ivankovo Reservoir (Upper Volga, Russia), a eutrophic reservoir which is becoming overgrown with macrophytes. The biotic component of freshwater ecosystems is formed by communities of multicellular and unicellular organisms and viruses in the water layer (plankton) and bottom sediments (benthos) and also macrophytes and autotrophic and heterotrophic organisms growing on their surface (epiphyton). The biomass of the biotic component of the Ivankovo Reservoir equaled 39,853 tons С. Plankton, benthos and macrophytes with epiphyton equaled 3.6%, 41.6% and 54.8% of the total biomass respectively. We determined the contribution of higher aquatic plants, algae, cyanobacteria, heterotrophic bacteria, viruses, protozoans, multicellular invertebrates and fish to the formation of total biomass. The largest share was taken up by higher aquatic plants (54.5%). The second largest share was taken by heterotrophic bacteria (37.4%), most of which live in the bottom sediments. The high concentration of bacteria and invertebrates in the bottom sediments indicate significant provision of the organic substrates from the water column. The biomass of fish, the highest trophic link in the reservoir, equaled 15.0% of the biomass of their potential food substrates, invertebrate animals, and 0.7% of the total biomass of the biotic component. The greater part of the autochthonous organic compound in the reservoir is formed as a result of activity of phytoplankton, which provides 69.4% of total primary production of macrophytes, phytoepiphyton, phytoplankton and phytobenthos. The total primary production during the vegetation period was approximately forty times higher than the annual production of the fish. Currently, the share in the phytoplankton of large colonial cyanobacteria not consumed by zooplankton, the share of non-heterocystic species of cyanobacteria capable of heterotrophic feeding and the share of mixotrophic flagellates is increasing. Eutrophication of the reservoir is significantly stimulated by the development of macrophytes, and, presumably, the contribution of macrophytes to the total primary production of the reservoir will continue to increase

Analysis of magnesium and levofloxacin complex compounds lipophilicity

The purpose of the study is to evaluate lipophilicity of complex compounds of levofloxacin with magnesium.Цель исследования – оценка липофильности комплексных соединений левофлоксацина с магнием

Beam-target helicity asymmetry for γ→n→→π−p in the N*resonance region

We report the first beam-target double-polarization asymmetries in the γ þ nðpÞ → π− þ pðpÞ reaction spanning the nucleon resonance region from invariant mass W ¼ 1500 to 2300 MeV. Circularly polarized photons and longitudinally polarized deuterons in solid hydrogen deuteride (HD) have been used with the CEBAF Large Acceptance Spectrometer (CLAS) at Jefferson Lab. The exclusive final state has been extracted using three very different analyses that show excellent agreement, and these have been used to deduce the E polarization observable for an effective neutron target. These results have been incorporated into new partial wave analyses and have led to significant revisions for several γnN* resonance photocouplings

Precision measurements of g1g_1 of the proton and the deuteron with 6 GeV electrons

The inclusive polarized structure functions of the proton and deuteron, g1p and g1d, were measured with high statistical precision using polarized 6 GeV electrons incident on a polarized ammonia target in Hall B at Jefferson Laboratory. Electrons scattered at lab angles between 18 and 45 degrees were detected using the CEBAF Large Acceptance Spectrometer (CLAS). For the usual DIS kinematics, Q^2>1 GeV^2 and the final-state invariant mass W>2 GeV, the ratio of polarized to unpolarized structure functions g1/F1 is found to be nearly independent of Q^2 at fixed x. Significant resonant structure is apparent at values of W up to 2.3 GeV. In the framework of perturbative QCD, the high-W results can be used to better constrain the polarization of quarks and gluons in the nucleon, as well as high-twist contributions