Synthesis and study of complexes of the novel Russian antiviral drug Camphecene with pentacyclic triterpenes of licorice

For the first time, the complexation of pentacyclic triterpenes of licorice (glycyrrhizic acid (GA) and its aglycone, glycyrrhetinic acid (GLA)) with the novel Russian antiviral drug Camphecene (Camph) was investigated. The complexes obtained at different molar ratios were studied using both UV/Vis spectroscopy and mass spectrometry (ESI MS). Formation of the host:guest complexes were registered: GA and GLA molecular complexes (Camph+2GA; Camph+2GLA) with stability constants К = 6.94 106 М-2 and К = 2.89 106 М-2, respectively. The research results demonstrate a considerable potential of ESI MS as a technique for simple and fast detection of formation of the complexes of GA /GLA and the novel drugs

Deep Learning for Spatiotemporal Anomaly Forecasting: A Case Study of Marine Heatwaves

Spatiotemporal data have unique properties and require specific considerations. Forecasting spatiotemporal processes is a difficult task because the data are high-dimensional, often are limited in extent, and temporally correlated. Hence, we propose to evaluate several deep learning-based approaches that are relevant to spatiotemporal anomaly forecasting. We will use marine heatwaves as a case study. Those are observed around the world and have strong impacts on marine ecosystems. The evaluated deep learning methods will be integrated for the task of marine heatwave prediction in order to overcome the limitations of spatiotemporal data and improve data-driven seasonal marine heatwave forecasts

Prediction of outcomes of cytomegalovirus infection of the initial few months of life

The research was based on the data of 155 newborns with manifestations of cytomegalovirus infection (CMV) appeared in the initial few months of life and on the results of the 7-years follow-up of this group of patients. While studying the courses and outcomes of CMV appeared in the initial few months of life in 155 patients of the tender age the disability is set for 22 (14,2 %) children. On the basis of discriminant analysis with the use of the most significant factors the model of prognosis of the outcomes of the carried infection was created in severe residual-organic disorders, dictating the necessity of determination of disability for children. This model can predict development of severe organ pathology in children of tender age after citomegalovirus infection appeared in the initial few months that will allow to select patients with high probability of disability and to realize for them individual rehabilitation measures to save their health in proper time

The results of follow-up study of children who had active cytomegalovirus infection during the first months of life

Follow-up observation of 44 patients who had cytomegalovirus infection (CMV) in the first months of life with the assessment of the health status of these children in terms was conducted during more than 7 years (control group consisted of healthy children). The study of course and outcomes of CMV infection of the first months of life showed that up to school period 43 % of patients had formed chronic pathology and 14,2 % of children at early age formed group of "children with disability". The structure formed chronic pathology up to school period in almost all parameters values. In main group central nervous system pathology occurred 4 times more often (р < 0,001), allergy - 3 times more often (p = 0,05), the pathology of the cardiovascular system - 3 times more often, endocrine pathology - 3 times more often, pathology of gastrointestinal tract and hepatobiliary system - 5 times more often

Synthesis and study of complexes of the novel Russian antiviral drug Camphecene with Plant’s Flavonoids

Received: 01.03.2021. Revised: 22.04.2021. Accepted: 27.04.2021. Available online: 28.04.2021.The authors are grateful to the Corresponding Member of the RAS, Doctor of Chemical Sciences, Professor of NIOCH SB RAS Salakhutdinov N.F. for the kindly provided scaffold monoterpenoid Camphecene.Traditionally, glycyrrhizic acid has been used to form polydentate complexes. For the first time in the presented paper, the complexation of the Plant’s Flavonoids (Quercetin (Qu) and its glycoside - Rutin (Rut)) with the novel Russian antiviral drug Camphecene (Camph) was investigated. The complexes obtained at different molar ratios were studied using UV/Vis spectroscopy. Formation of the host: guest complexes were registered: Qu and Rut molecular complexes (Camph+2Qu; Camph+2Rut) with a stability constant K = 3.3·108 M-2. Comparison of the binding constants of the obtained complexes shows that the efficiency of Camphecene complexation with the participation of flavonoids is more efficient than with the participation of triterpenoids. Besides, it was found that the complexes of Camphecene with the quercetin and rutin are soluble in water, in contrast to the complexes with triterpenoids, which makes it possible to increase the bioavailability of both Camphecene and flavonoids. The obtained results demonstrate the high potential of flavonoids Qu and Rut to the development of novel pharmaceutical forms using the example of Camphecene in the form of molecular complexes, as the novel forms of delivery.This work was supported by the Ministry of Science and Higher Education of the Russian Federation (State assignment in the field of scientific activity, project No 0852-2020-0031) and the Russian Foundation for Basic Research (RFBR, grant no. 19-33-90211-Aspiranty)

The role of active herpesvirus infection in the formation of atopic dermatitis and psoriasis

The results of previous studies suggest the involvement of herpes viruses in the development of atopic dermatitis and psoriasis, but there is no convincing evidence. Aim of the study was to evaluate the role of active herpesvirus infection in exacerbation of atopic dermatitis and psoriasis. Material and methods. The analysis of the incidence of infectious mononucleosis, genital herpes, cytomegalovirus infection, skin and subcutaneous tissue diseases, atopic dermatitis, psoriasis in Russia in 2000–2020 was carried out. 92 blood donors and 97 patients with skin and subcutaneous tissue diseases (44 with atopic dermatitis and 53 with psoriasis) were examined for the presence of immunoglobulins M and G to herpes simplex viruses (HSV1,2), Epstein-Barr (EBV), cytomegalovirus (CMV), human herpes type 6 (HНV6). Results and discussion. A significant strong direct correlation was revealed between the incidence of genital herpes and diseases of the skin and subcutaneous tissue (p = 0.85), atopic dermatitis (p = 0.85); infectious mononucleosis and psoriasis (p = 0.85). The frequency of detection of IgM to HSV1,2, in total, IgG EA and IgM VCA EBV in the group of patients is significantly higher than in donors (p &lt; 0.05). In patients with atopic dermatitis, IgM to HSV1 were detected significantly more often than in individuals with psoriasis, and markers of active EBV infection were significantly less common. The presence of IgM HSV-1 statistically significantly increases the incidence of atopic dermatitis (relative risk (RR) = 2.3, 95 % confidence interval (95 % CI) 1.6–3.3)), IgM VCA and IgG EA EBV – the incidence of psoriasis (RR = 2.3, 95 % CI 1.5–3.3). Conclusions. It has been shown for the first time that active HSV1,2 infection is a trigger factor for the development of atopic dermatitis, EBV infection is psoriasis

Architecture of composite multilayer semiconductor nanostructures

The problem of ensuring the operational parameters of composite multilayer semiconductor nanoscale structures at the design technology stages is solved. A mathematical model based on the physics of processes occurring in the structure during operation is developed. The problem is solved for the resonant-tunnelling AlGaAs nanoheterostructures

ДЕЙСТВИЕ ЭКЗОГЕННЫХ СТЕРОИДНЫХ ГЛИКОЗИДОВ НА ПРОЯВЛЕНИЕ ИНБРЕДНОЙ ДЕПРЕССИИ РАСТЕНИЙ СВЁКЛЫ СТОЛОВОЙ В УСЛОВИЯХ ЗАЩИЩЕННОГО ГРУНТА

The protected cultivation technology, through which the various inbred generations with the combination of economic valuable traits and different level of sterility can be produced, is used in order to accelerate the breeding program. However, there is a negative effect of inbreeding depression and self-incompatibility can often occur and cause the loss of valuable breeding forms. The aim of the work was to study the influence of steroidal glycosides capsicoside (SGC) on exhibition of CMS, and morphobiological parameters of 13 inbred generations that were produced from fertile plant and partly sterile plants with level of sterility 10% and 50%. The seeds were soaked for 24 hours in water solution of SGC with concentration 10-3%, and in water control. Then the seeds were dried up and sown in the greenhouse. The stecklings and roots obtained were vernalized at 3-5Co. Mother plants were grown under 18 hour photoperiod in greenhouse with supplementary lighting. Inbreeding seeds were obtained in individual cloth isolators. It was shown that for all generations the treatment with SGC improved the seed germination (4-8% more), increased the root index and its length (12-24% more), decreased betanin content (22-48% less) in comparison with control. The action of SGC on the other morphological and biochemical traits such as height of leaf rosette, leaf number, plant and root weight, head size, number of generative buds, and nitrate content was defined by the level of sterility of mother plant. The most expressed effect for all traits mentioned was seen in inbreeding generations of sterile plants with high level of sterility. After action effect of seed treatment with SGC on development of seed plants from inbreeding generations, not depending on sterility level of mother plants, showed the positive influence on plant habitus of seed mother plants, decreasing the plant height, but increasing stem number and functional parameters of microgametophyte in fertile plants. This caused the improved seed formation 1.6-2.2 times higher during inbreeding process and increased the portion of plants with high productivity. The SGC had no effect on exhibition of CMS trait. Thus, the SGC is worth using in valuable highly inbreeding forms inclined to self-sterility, in order to enlarge the genetic variation and output of divers generations. However, it should be taken into consideration that the complicated effect on the plant traits can be noticed in inbreeding generation of red beet and may have an effect on the result of selection process at the root stage.Для ускорения  селекционного  процесса  используют защищенный грунт, что дает возможность   получать широкий спектр инбредных потомств с различным  сочетанием селекционно значимых признаков и степени стерильности растений. Однако  негативное влияния  инбредной депрессии  и явления самонесовместимости часто приводит к потере ценных селекционных форм. Цель работы – изучение влияния стероидного гликозида  капсикозида  (СГ) на проявление  признака  ЦМС  и морфо-биологические  параметры инбредных потомств I3   от фертильного и частично-стерильных исходных  растений со степенями стерильности 10% и 50%. Семена замачивали  на 24 часа в водном растворе СГ концентрацией 10-3% (контроль – вода),  затем подсушивали и высевали в теплице. Полученные  штеклинги и корнеплоды яровизировали при 3…5°С.  Семенные  растения выращивали  при 18-часовом световом периоде в теплице  при досветке. Инбредные  семена  получали  под индивидуальными бязевыми изоляторами. Установлено, что обработка семян  СГ во всех  потомствах способствовала повышению лабораторной всхожести (на 4-8%), увеличению длины (на 12-24%) и  индекса формы корнеплодов; повышению содержания в них суммы сахаров  (в среднем на 25%) и снижению количества бетаина (на 22-48%)  относительно контроля. Направленность действия СГ на другие морфологические признаки (высота листовой розетки, число  листьев, масса  растений и корнеплода, размер головки, число генеративных почек) и биохимические  параметры (накопление  нитратов)  определялась степенью стерильности исходного  растения.   Наиболее  выраженный   стимулирующий   эффект по изученным  признакам   отмечали в инбредном потомстве   ms-растения     с   высокой степенью    стерильности. Последействие обработки семян  СГ на развитие семенных  растений инбредных потомств,  независимо от уровня   стерильности  исходных форм, выразилось в их положительном влиянии на габитус семенного растения (снижение высоты при увеличении  числа стеблей) и функциональные  параметры микрогаметофита фертильных пыльников.   Это способствовало  повышению завязываемости семян при инбридинге (в 1,6-2,2  раза) и увеличению  доли  более  продуктивных растений. На характер проявления  признака  ЦМС обработка семян СГ не повлияла. То есть, СГ целесообразно   использовать при работе с ценными формами глубоких инбридингов, склонных к самостерильности, что позволит расширить спектр  и увеличить выход генетически разнообразного потомства. Однако  следует  учитывать их неоднозначное  действие на проявление  хозяйственно ценных признаков  в различных инбредных потомствах свеклы  столовой,  что может влиять на результативность отбора на стадии корнеплода

Динамика выживаемости больных муковисцидозом в Москве и Московской области за периоды 1992–2001 и 2002–2011 гг.

Summary. The aim of this study was to evaluate changes in survival of patients with cystic fibrosis (CF) in Moscow and Moscow region and to determine factors influencing the survival. We analyzed outpatient medical records of patients followed-up in Moscow CF centers by the 1st of January, 2002, and by the 1st of January, 2012.Median survival for 2002–2012 was 37.2 years; this was significantly higher that the similar value for the previous decade (25.9 years). A total number of CF patients was more than doubled due to increased survival and improved diagnosis of the disease.Improved survival was due to improved work of CF centers and to implementation of effective medications, primarily dornase alfa (Pulmozyme) and some inhaled and systemic antibiotics and macrolides in subinhibitory concentrations, in routine clinical practice. Gram-negative infection, especially Burkholderia cepacia, was shown to decrease survival in CF patients in contrary to "mild" mutations that are better prognostic factors.Резюме. Целью работы явилось определение динамики числа и выживаемости больных муковисцидозом (МВ) в Москве и Московской области, а также определение факторов, влияющих на выживаемость. Были проанализированы амбулаторные карты больных, состоящих на учете в московских центрах муковисцидоза на 01.01.2002 и 01.01.2012.Медиана выживаемости больных за периоды 2002–2011 гг. составила 37,2 года, что достоверно выше, чем аналогичный показатель за предыдущее 10-летие – 25,9 года. Отмечено более чем 2-кратное увеличение общего количества больных, обусловленное ростом выживаемости и улучшением диагностики.Увеличение выживаемости обусловлено оптимизацией работы центров МВ и началом рутинного применения различных эффективных медикаментозных препаратов, прежде всего дорназы альфа (Пульмозим), а также некоторых ингаляционных и системных антибиотиков, макролидов в субингибирующих концентрациях. Показано, что инфицирование грамотрицательной инфекцией, особенно Burkholderia cepacia, снижает выживаемость пациентов с МВ, а наличие "мягкого" генотипа является более благоприятным прогностическим фактором