Some biological characteristics of the Batrachedra amydraula Meyrick (Lepidoptera: Batrachedridae) on main varieties of dry and semi-dry date palm of Iran

Although Iran is the leading producer of dates in the world, only 10% of its product is exported. One of the factors which limits exportation is postharvest pests. Lesser date moth (Batrachedra amydraula) is one of these postharvest pests. This pest is found in all date palm plantations of Iran. The biology of this pest has not been studied under laboratory conditions on main varieties of stored date in Iran. In this research some biological characteristics including survivorship, developmental time, fecundity, oviposition and postoviposition period, and longevity of adults on date palm varieties Zahedi, Ghasb, Rabbi, Deyri and a semi-artificial diet were studied. All experiments carried out at constant temperature room (30 ± 0.5 °C and 55 ± 5% r.h.). Highest survivorship of egg and combined larval and pupal stages were 86 and 85% respectively on semi-artificial diet. Shortest total development time from egg to adult was 43 days for males and 43 days for females on semi-artificial diet. Longest total development time was 69 days for male and 65 days for female on Ghasb variety. The highest (45 eggs per female) and lowest (25 eggs per female) fecundity occurred on the Deyri variety and semi-artificial diet, respectively. Results of this study would be useful for making pest management decisions in date palm storage.Keywords: Lesser date moth, Batrachedra amydraula, Biology, Date pal

Biological characteristics of Cadra cautella (Lep.: Pyralidae) on different varieties of stored date palm fruit of Iran

The postharvest pests, including the dried fruit moth Cadra cautella Walker, are important limiting factors in the exportation of dates. The biology of C. cautella, its survivorship, developmental time, fecundity, oviposition, post oviposition period, as well as longevity of the adults were studied on four date palm varieties of Zahedi, Piarom, Rabbi, and Deyri under laboratory conditions in Iran. The experiments were carried out at constant temperature room (29 ± 1 °C, 60 ± 5% RH and photoperiod 16L: 8D hrs.). The highest survivorship of egg and larval stages was recorded 92.66%, 85.89% on Zahedi and 100% for pupal stage on Rabbi. The shortest larval period was 32.93 ± 1.41 days and 33.37 ± 1.87 days on Deyri and Zahedi respectively. The shortest total development time for males from egg to adult was 40.91 ± 1.15 days and 40.97 ± 1.59 days on Zahedi and Deyri respectively and for females was 43.76 ± 2.47 days on Deyri. The longest total development time for males was 47.61 ± 1.50 days on Piarom and for female was 55.88 ± 2.31 and 55.37 ± 3.87 days on Piarom and Rabbi respectively. The highest (247.00 ± 8.53 eggs per female) and lowest (147.40 ± 12.06 eggs per female) fecundity occurred on Zahedi and Piarom, respectively. The date palm varieties Zahedi, Deyri, Rabbi and Piarom are found to be on preferrence order for C. cautella. The results of this study would be useful for planning pest management strategies in date palm fruit storages

Standardization of a galactogogue herbal mixture based on its total phenol and flavonol contents and antioxidant activity

Background and objectives: Dairy companies always try to increase the quantity and quality of milk production. According to the positive impact of different plants on milk production of cattle, this study was carried out to standardize a galactogogue herbal mixture based on its total phenol and flavonol contents and antioxidant activity.  Methods: A mixture of Galega officinalis L. and Nigella sativa L. powders (4:1 w/w), which had shown milk stimulating activity on Holstein cows in a previous study, was extracted with hydro alcoholic solvent (80% aq. methanol) and its 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity and total phenol and total flavonol contents were measured according to standard methods. Results: The inhibition concentration 50% (IC50 ) value of radical scavenging activity in DPPH test and the total phenol and total flavonol contents were found to be 154.29 µg/mL, 77.720±0.104 µg of gallic acid equivalent (GAE) and 0.231±0.018 µg of quercetin equivalent (QE) per mg of dry extract, respectively. Conclusion: This herbal extract can be standardized based on its antioxidant activity or total phenol and total flavonol contents and the standardized extract can be formulated as a veterinary galactogogue supplement

Standardization of a galactogogue herbal mixture based on its total phenol and flavonol contents and antioxidant activity

Abstract Background and objectives: Dairy companies always try to increase the quantity and quality of milk production. According to the positive impact of different plants on milk production of cattle, this study was carried out to standardize a galactogogue herbal mixture based on its total phenol and flavonol contents and antioxidant activity. Methods: A mixture of Galega officinalis L. and Nigella sativa L. powders (4:1 w/w), which had shown milk stimulating activity on Holstein cows in a previous study, was extracted with hydro alcoholic solvent (80% aq. methanol) and its 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity and total phenol and total flavonol contents were measured according to standard methods. Results: The inhibition concentration 50% (IC 50 ) value of radical scavenging activity in DPPH test and the total phenol and total flavonol contents were found to be 154.29 µg/mL, 77.720±0.104 µg of gallic acid equivalent (GAE) and 0.231±0.018 µg of quercetin equivalent (QE) per mg of dry extract, respectively. Conclusion: This herbal extract can be standardized based on its antioxidant activity or total phenol and total flavonol contents and the standardized extract can be formulated as a veterinary galactogogue supplement

Cytotoxic activity of the essential oil of Salvia verticillata L.

Salvia is one of the largest genera of  Lamiaceae family. Several species of this genus are perfumed and wealthy in essential oils. Some of them are used in industry, pharmacy and aromatherapy. They have shown different biological effects such as antibacterial and antioxidant activity. For the present study, Salvia verticillata L. was collected from Shahrestanak, Mazandaran, Iran. Hydrodistilled essential oil from the aerial parts of this plant was obtained with a Clevenger type  apparatus  and was analyzed by GC and GC/MS. Moreover, the cytotoxic activity of the essential oil was investigated against HT-29 (colon adenocarcinoma), Caco-2 (colorectal adenocarcinoma), T-47D (breast ductal carcinoma) and NIH-3T3 (Swiss mouse embryo fibroblast) cell lines by MTT test. 59 components were characterized from the oil with trans-caryophyllene (24.40%), β-phellandrene (9.08%), α-humulene (8.61%), bicyclogermacrene (6.32%), spathulenol (5.89%) and β-pinene (5.00%) as the major constituents. These compounds represented 97.67% of the essential oil and included monoterpenes (34.83%) and sesquiterpens (61.84%). The results of the cytotoxicity assay demonstrated that the essential oil of S. verticillata showed higher cytotoxic effect on Caco-2 cell line

Pareto Design of Decoupled Sliding-Mode Controllers for Nonlinear Systems Based on a Multiobjective Genetic Algorithm

This paper presents Pareto design of decoupled sliding-mode controllers based on a multiobjective genetic algorithm for several fourth-order coupled nonlinear systems. In order to achieve an optimum controller, at first, the decoupled sliding mode controller is applied to stablize the fourth-order coupled nonlinear systems at the equilibrium point. Then, the multiobjective genetic algorithm is applied to search the optimal coefficients of the decoupled sliding-mode control to improve the performance of the control system. Considered objective functions are the angle and distance errors. Finally, the simulation results implemented in the MATLAB software environment are presented for the inverted pendulum, ball and beam, and seesaw systems to assure the effectiveness of this technique

DNA barcoding, phylogeny and phylogeography of the cyst nematode species of the Schachtii group from the genus Heterodera (Tylenchida: Heteroderidae)

Cyst-forming nematodes of the genus Heterodera are highly derived and economically important plant parasites. The Schachtii group of this genus is one of the largest ones with a total of 18 species parasitising dicotyledons. In this study, we provided comprehensive phylogenetic analyses of several hundred COI and ITS rRNA gene sequences of selected species from the Schachtii group, including H. betae, H. cajani, H. ciceri, H. galeopsidis, H. glycines, H. medicaginis, H. mediterranea, H. schachtii, H. sonchophila and H. trifolii, using Bayesian inference, maximum likelihood, and statistical parsimony. One hundred and twenty four new COI, 57 ITS rRNA and eight hsp90 gene sequences from 81 nematode populations collected in 19 countries were obtained in this study. Our study showed that the ITS rRNA gene has limited discrimination power compared to the COI gene. However, our analysis also revealed that partial COI gene sequences were identical for H. trifolii, H. betae and H. galeopsidis. Based on the results of phylogeographical analysis and age estimation of clades with a molecular clock approach, it was hypothesised that the majority of the Schachtii group species originated and diversified in the Mediterranean Basin biodiversity hotspot during the Pleistocene and then dispersed from this region across the world. The Sino-Japanese Floristic Region is likely one of the centres of diversification for the soybean cyst nematode, which showed distinct population structure. The possible role of hybridisation and polyploidisation in the evolution of species of the Schachtii group is discussed.This work was sponsored by USDA APHIS Farm Bill grants: AP18PPQS&T00C201/18-0430-000-FR and AP20PPQS&T00C078/20-0256-000-FR.Peer reviewe

Characterisation of populations of Longidorus orientalis Loof, 1982 (Nematoda: Dorylaimida) from date palm (Phoenix dactylifera L.) in the USA and other countries and incongruence of phylogenies inferred from ITS1 rRNA and coxI genes

Needle nematode populations of Longidorus orientalis associated with date palm, Phoenix dactylifera, and detected during nematode surveys conducted in Arizona, California and Florida, USA, were characterised morphologically and molecularly. The nematode species most likely arrived in California a century ago with propagative date palms from the Middle East and eventually spread to Florida on ornamental date palms that were shipped from Arizona and California. This is the first validated continental record of this needle nematode species in the USA and the Americas. The USA populations of L. orientalis contained a small number of males that were not reported in the original description and are herein described. Longidorus orientalis was able to survive for at least 4 years at very low numbers in the warm and humid environment of Florida on date palms imported from California and Arizona. Association of L. orientalis with L. africanus was observed in all of the surveyed sites, indicating that date palm is a host of both nematodes. Phylogenetic relationships of L. orientalis with closely related Longidorus species, in addition to relationships between populations of L. orientalis from the USA, Greece, Iran and Spain, were inferred from the analyses of D2-D3 of 28S rRNA, ITS1 rRNA and partial coxI gene sequences. The PCR-D2-D3 expansion segments of 28S rDNA-RFLP diagnostic profile is provided. Longidorus orientalis populations display a high level of intraspecific variation (up to 15.5%) in coxI mtDNA sequences. Analysis of phylogenetic relationships of nematode populations revealed incongruence of the ITS1 rRNA and coxI mtDNA gene trees, which might be the result of selective introgression of mtDNA through gene flow between previously isolated populations introduced simultaneously into new geographical regions.Peer reviewe

Caracterización morfológica y molecular de nematodes picadores de la raíz (Longidorus spp.) sobre palmera datilera en Arizona, California y Florida

Trabajo presentado en la 47th Annual Meeting of the Organization of Nematologists of Tropical America, celebrada en Varadero (Cuba) del 17 al 22 de mayo de 2015.For decades, ornamental growers in Florida have imported date palms (Phoenix dactylifera) from Arizona and California. Needle nematodes (Longidorus spp.) with conoid and hemispherical tails have been detected consistently in these imported palms in Florida. The populations with conoid tails have been identified routinely as Longidorus africanus and those with hemispherical tails as L. belloi, L. belondiroides, and L. orientalis. Recent molecular and morphological analyses have confirmed the identification of L. africanus and also that of L. orientalis, reported by R. Esser, in Florida, in 1995 on date palms from California. These findings disprove the reports of detection of L. belloi and L. belondiroides, not found again, and validate that of L. orientalisas a continental record in the United States and the Americas. Longidorus africanus is a known parasite of vegetables in Southern California, whereas L. orientalis is a parasite of date palm in the Middle East. The populations of L. orientaliscontained a few males, not reported in the original description. Longidorus orientalis was commonly found in association with L. africanus and was able to survive for at least four years, at low densities in warm and humid environments of Florida, on transplanted date palms imported from California and Arizona. Phylogenetic relationships of L. orientalis with closely related Longidorus species were reconstructed using D2-D3 of 28S rRNA, ITS1 rRNA, and partial coxI gene sequences. The PCR-D2-D3 of 28S rDNA-RFLP diagnostic profile was obtained and proposed for the identification of this species. Longidorus orientalis shows high intraspecific variation (up to 15.5%) in coxI mtDNA sequences. Incongruence between ITS1 rRNA and coxImtDNA gene trees was noticed from the analysis of phylogenetic relationships between L. orientalispopulations, indicating selective introgression of mtDNA through gene flow as a consequence of hybridization of populations from different origin.N