Структурные изменения в поджелудочной железе в процессе деадаптации к высокогорью

Study of the pancreas of the rats at different periods deadaptation to valley, after 60 day adaptations to mountain has revealled significant change, which at the first periods of the study (7, 15 day) were concluded in edema stroma, inflammatory round cells, plethora container and grousing connective tissue between share and around acinus. At late periods of the study dominated change, expressing grousing connective tissue stroma ferrics with atrophy acinus parenchyma and dystrophic change acinous hutches (picnosis, vacuolization of cytoplasm). Deadaption has found the weakening an compensatory reaction with prevalence destruction processes in pancreas.Исследование поджелудочной железы крыс в различные сроки деадаптации к долине после дневной 60- адаптации к горам выявило значительные изменения которые в первые сроки исследования е е , (7- , 15- сут) заключались в отеке стромы воспалительной круглоклеточной инфильтрации полнокровии сосудов и раз , , - растании соединительной ткани между долей и вокруг ацинусов В поздние сроки исследования преоблада . - ли изменения выражающиеся разрастанием соединительной ткани стромы железы с атрофией ацинозной , паренхимы и дистрофическими изменениями ацинозных клеток пикноз вакуолизация цитоплазмы Деадап ( , ). - тация обнаружила ослабление компенсаторных реакций с преобладанием деструктивных процессов в под - желудочной железе

GC-based chemoprofile of lipophilic compounds in Altaian Ganoderma lucidum sample

The presented data contains information about component composition of lipophilic compounds in Ganoderma lucidum fungal body sample obtained using gas chromatography and subsequent mass spectrometry

Gas chromatography-mass spectrometry in the taxonomy of Miscanthus

Chemotaxonomy as a system approach deals with intra- and interspecific polymorphism of a group of taxa in order to clarify their taxonomic positions or to select material for selection or introduction. In this study we performed chemotaxonomic analysis of specimens of Miscanthus sinensis and M. sacchariflorus collected in the Russian Far East and of hybrid plants of both natural and artificial origin. We found 153 substances and identified 143 of them in extracts of eleven Miscanthus plants by gas chromatography-mass spectrometry (GC-MS). These substances can be grouped into alkanes (20 compounds), fatty acids (34), phenols (13), sterols (18) toсopherols (8), norterpenoids (12), and phytols (13), as well as their derivatives. The main components of the extracts of miscanthus samples are fatty acids and their derivatives (total content 19.94–41.02 %), dominated by palmitic and linolenic acids, and sterols (mainly β-sitosterol, stigmasterol, and α-amyrin), which constitute 17.15–31.73 %. The values of the CPI “oddness index” for the alkane components of the extracts were within 1.55–7.18, with extracts from leaves of the Far Eastern samples characterized by the lower half of this range (1.55–2.74), while extracts from leaves of hybrids fell to the upper half (5.78–7.18). Principal component analysis of extraction profiles allowed us to separate three distinct clusters: M. sinensis, M. sachariflorus, and their hybrids, as well as to verify the origin of one of the natural hybrids. The results of chemotaxonomic analysis mostly matched those of DNA sequencing of a fragment of the plastid genome, which, moreover, allowed us to identify the species nature of the maternal plants used to obtain these hybrids. Chemotaxonomic analysis using GC-MS was found to be an efficient additional technique to delimit various morphological forms of M. sinensis, M. sachariflorus, and their hybrids

An integrated method for taxonomic identif ication of microorganisms

For accurate species-level identification of microorganisms, researchers today increasingly use a combination of standard microbiological cultivation and visual observation methods with molecular biological and genetic techniques that help distinguish between species and strains of microorganisms at the level of DNA or RNA molecules. The aim of this work was to identify microorganisms from the ICG SB RAS Collection using an integrated approach that involves a combination of various phenotypic and genotypic characteristics. Key molecular-genetic and phenotypic characteristics were determined for 93 microbial strains from the ICG SB RAS Collection. The strains were characterized by means of morphological, physiological, moleculargenetic, and mass-spectrometric parameters. Specific features of the growth of the strains on different media were determined, and cell morphology was evaluated. The strains were tested for the ability to utilize various substrates. The strains studied were found to significantly differ in their biochemical characteristics. Physiological characteristics of the strains from the collection were identified too, e. g., the relationship with oxygen, type of nutrition, suitable temperature and pH ranges, and NaCl tolerance. In this work, the microorganisms analyzed were combined into separate groups based on the similarities of their phenotypic characteristics. This categorization, after further refinement and expansion of the spectrum of taxa and their metabolic maps, may serve as the basis for the creation of an “artificial” classification that can be used as a key for simplified and quicker identification and recognition of microorganisms within both the ICG SB RAS Collection and other collections

Collection of microorganisms of ICG SB RAS as a genetic resource for biotechnology

Genetic knowledge of microorganisms plays a critical role in the creation of new biotechnologies, since the effectiveness of any biotechnology is determined by the particular qualities of the structurally functional organization of molecular-genetic systems and their components used for the production of targeted products. Collections of microbial cultures play a decisive role in mobilizing biological resources and make it possible to form a solid base for genetic, molecular biological and biotechnological research. The aim of this work was to assess the key molecular-genetic and phenotypic characteristics of strains of the collection of microorganisms created in the “FRC Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences” as a genetic resource for biotechnology. Thirty strains of microorganisms of the collection were isolated by employees of the FRC ICG SB RAS from extreme natural ecosystems, the key molecular-genetic and phenotypic characteristics were described using modern methods of molecular biology and mass-spectrometry. DNA isolation and the sequencing of 16S rRNA gene sequences were performed. The strains of the collection were characterized by morphological, physiological, moleculargenetic and mass-spectrometric characteristics. The particular qualities of growing of strains on different substrates have been established, the study of cell morphology has been carried out. The physiological characteristics of the strains of the collection have been established: the attitude to oxygen, the type of nutrition, the range of temperature and pH, the attitude to NaCl and others. Different resistance of strains to antibiotics has been established. The creation of personal mass spectra of protein profiles of the studied strains of the collection was carried out. The resulting DNA sequences of the strains are deposited in the GenBank. The chemotaxonomic characteristics of strains have been determined. The biotechnological properties of the strains were assessed, the amount of metabolites (ethanol, lactic and acetic acids) in the culture liquid was determined. The value of the collection of microorganisms of the FRC ICG SB RAS as a genetic resource for biotechnology and bioengineering is determined not only by the species diversity of its strains, but also by a wide range of their area isolation and by the depth of their characterization using the widest arsenal of both classical and modern methods (including methods of genomics, proteomics, transcriptomics and bioinformatics)

A medical error: does law help or hinder

A medical error: does law help or hinder / Nataliia V. Nikitchenko, Andrii M. Khankevych, Dmytro V. Slynko and oth. // Wiadomości Lekarskie. - Warsaw, 2019. - Т. LXXII, nr 4. - P. 697-701.Системи охорони здоров'я та політика охорони здоров'я в Європейському союзі стають все більш взаємопов'язаними, а також більш складними. Цей взаємозв'язок піднімає багато питань політики охорони здоров'я, включаючи якість охорони здоров'я. Помилки в медичної допомоги можуть виникати в будь-якому місці системи охорони здоров'я - в лікарнях, кабінетах лікарів, будинках для людей похилого віку, аптеках - і в будь-якій частині процесу лікування, пов'язаної з неправильним лікуванням. Метою статті є розробка адекватних теоретичних і науково-практичних пропозицій щодо модернізації правового регулювання для захисту прав пацієнтів, спрямованих на дотримання конституційних прав і свобод.Introduction: Health systems and health policies across the European Union are becoming more and more interconnected and also more complex. This increased interconnection raises many health policy issues, including health care quality. Mistakes in medical care can occur anywhere in the health care system - at hospitals, doctor's offices, nursing homes, pharmacies, or patients' homes - and in any part of the treatment process involving wrong medication, improper treatment, or incorrect or delayed test results. The aim of the article is to develop adequate theoretical and scientific-practical proposals for the modernization of the legal regulation to protect patients' rights aimed at observance of constitutional rights and freedoms. Patients and methods: Materials and methods: In order to obtain the results the analysis of medical, labor and civil law norms are investigated. The article uses analysis and synthesis methods, as well as a comparative legal method. Results: Review: A number of proposals are given for improving legislation in the area of eliminating obstacles to provision of qualitative primary care / medical-preventive care, prevention of formal attitude towards the patient, the implementation of preventive protection measures which should notify in advance about the violation of the law in the medical sphere. Conclusion: Conclusions: Ukraine urgently needs a legal mechanism to protect the rights of patients; it will become a systemic phenomenon and will consist of legal means, forms, ways by which the restoration of violated patients' rights is provided, the support of protected interests by the law is maintained, legal disputes are resolved and other obstacles to realization of patients' rights are overcome.Системы здравоохранения и политика здравоохранения в Европейском союзе становятся все более взаимосвязанными, а также более сложными. Эта возросшая взаимосвязь поднимает многие вопросы политики здравоохранения, включая качество здравоохранения. Ошибки в медицинской помощи могут возникать в любом месте системы здравоохранения - в больницах, кабинетах врачей, домах престарелых, аптеках - и в любой части процесса лечения, связанной с неправильным лечением. Целью статьи является разработка адекватных теоретических и научно-практических предложений по модернизации правового регулирования для защиты прав пациентов, направленных на соблюдение конституционных прав и свобод

Unpublished Mediterranean and Black Sea records of marine alien, cryptogenic, and neonative species

To enrich spatio-temporal information on the distribution of alien, cryptogenic, and neonative species in the Mediterranean and the Black Sea, a collective effort by 173 marine scientists was made to provide unpublished records and make them open access to the scientific community. Through this effort, we collected and harmonized a dataset of 12,649 records. It includes 247 taxa, of which 217 are Animalia, 25 Plantae and 5 Chromista, from 23 countries surrounding the Mediterranean and the Black Sea. Chordata was the most abundant taxonomic group, followed by Arthropoda, Mollusca, and Annelida. In terms of species records, Siganus luridus, Siganus rivulatus, Saurida lessepsianus, Pterois miles, Upeneus moluccensis, Charybdis (Archias) longicollis, and Caulerpa cylindracea were the most numerous. The temporal distribution of the records ranges from 1973 to 2022, with 44% of the records in 2020–2021. Lethrinus borbonicus is reported for the first time in the Mediterranean Sea, while Pomatoschistus quagga, Caulerpa cylindracea, Grateloupia turuturu, and Misophria pallida are first records for the Black Sea; Kapraunia schneideri is recorded for the second time in the Mediterranean and for the first time in Israel; Prionospio depauperata and Pseudonereis anomala are reported for the first time from the Sea of Marmara. Many first country records are also included, namely: Amathia verticillata (Montenegro), Ampithoe valida (Italy), Antithamnion amphigeneum (Greece), Clavelina oblonga (Tunisia and Slovenia), Dendostrea cf. folium (Syria), Epinephelus fasciatus (Tunisia), Ganonema farinosum (Montenegro), Macrorhynchia philippina (Tunisia), Marenzelleria neglecta (Romania), Paratapes textilis (Tunisia), and Botrylloides diegensis (Tunisia)

Structured pancreas changes in deadaptation process to high altitude

Study of the pancreas of the rats at different periods deadaptation to valley, after 60 day adaptations to mountain has revealled significant change, which at the first periods of the study (7, 15 day) were concluded in edema stroma, inflammatory round cells, plethora container and grousing connective tissue between share and around acinus. At late periods of the study dominated change, expressing grousing connective tissue stroma ferrics with atrophy acinus parenchyma and dystrophic change acinous hutches (picnosis, vacuolization of cytoplasm). Deadaption has found the weakening an compensatory reaction with prevalence destruction processes in pancreas