Capacity Building for Lavumisa Irrigation Development Project: process documentation

Water resource management / Multiple use / Project planning / Project management / Stakeholders / Agricultural cooperatives / Participatory management / Sugarcane / Irrigated farming / Water supply / Drinking water / Sanitation / Swaziland / Maplotini / Lavumisa Irrigation Development Project

Formal water rights in rural Tanzania: Deepening the dichotomy?

Water rights / Water law / Water scarcity / Water use / Water users’ associations / Irrigation water / Cost recovery

Small Aircraft RF Interference Path Loss

Interference to aircraft radio receivers is an increasing concern as more portable electronic devices are allowed onboard. Interference signals are attenuated as they propagate from inside the cabin to aircraft radio antennas mounted on the outside of the aircraft. The attenuation level is referred to as the interference path loss (IPL) value. Significant published IPL data exists for transport and regional category airplanes. This report fills a void by providing data for small business/corporate and general aviation aircraft. In this effort, IPL measurements are performed on ten small aircraft of different designs and manufacturers. Multiple radio systems are addressed. Along with the typical worst-case coupling values, statistical distributions are also reported that could lead to better interference risk assessment

Rapid hepatic clearance of full length CCN-2/CTGF: a putative role for LRP1-mediated endocytosis

This is the final version. Available on open access from Springer via the DOI in this record.CCN-2 (connective tissue growth factor; CTGF) is a key factor in fibrosis. Plasma CCN-2 has biomarker potential in numerous fibrotic disorders, but it is unknown which pathophysiological factors determine plasma CCN-2 levels. The proteolytic amino-terminal fragment of CCN-2 is primarily eliminated by the kidney. Here, we investigated elimination and distribution profiles of full length CCN-2 by intravenous administration of recombinant CCN-2 to rodents. After bolus injection in mice, we observed a large initial distribution volume (454 mL/kg) and a fast initial clearance (120 mL/kg/min). Immunosorbent assay and immunostaining showed that CCN-2 distributed mainly to the liver and was taken up by hepatocytes. Steady state clearance in rats, determined by continuous infusion of CCN-2, was fast (45 mL/kg/min). Renal CCN-2 clearance, determined by arterial and renal vein sampling, accounted for only 12 % of total clearance. Co-infusion of CCN-2 with receptor-associated protein (RAP), an antagonist of LDL-receptor family proteins, showed that RAP prolonged CCN-2 half-life and completely prevented CCN-2 internalization by hepatocytes. This suggests that hepatic uptake of CCN-2 is mediated by a RAP-sensitive mechanism most likely involving LRP1, a member of the LDL-receptor family involved in hepatic clearance of various plasma proteins. Surface plasmon resonance binding studies confirmed that CCN-2 is an LRP1 ligand. Co-infusion of CCN-2 with an excess of the heparan sulphate-binding protamine lowered the large initial distribution volume of CCN-2 by 88 % and reduced interstitial staining of CCN-2, suggesting binding of CCN-2 to heparan sulphate proteoglycans (HSPGs). Protamine did not affect clearance rate, indicating that RAP-sensitive clearance of CCN-2 is HSPG independent. In conclusion, unlike its amino-terminal fragment which is cleared by the kidney, full length CCN-2 is primarily eliminated by the liver via a fast RAP-sensitive, probably LRP1-dependent pathway.FibroGen, Inc

Rapid hepatic clearance of full length CCN-2/CTGF: a putative role for LRP1-mediated endocytosis

CCN-2 (connective tissue growth factor; CTGF) is a key factor in fibrosis. Plasma CCN-2 has biomarker potential in numerous fibrotic disorders, but it is unknown which pathophysiological factors determine plasma CCN-2 levels. The proteolytic amino-terminal fragment of CCN-2 is primarily eliminated by the kidney. Here, we investigated elimination and distribution profiles of full length CCN-2 by intravenous administration of recombinant CCN-2 to rodents. After bolus injection in mice, we observed a large initial distribution volume (454 mL/kg) and a fast initial clearance (120 mL/kg/min). Immunosorbent assay and immunostaining showed that CCN-2 distributed mainly to the liver and was taken up by hepatocytes. Steady state clearance in rats, determined by continuous infusion of CCN-2, was fast (45 mL/kg/min). Renal CCN-2 clearance, determined by arterial and renal vein sampling, accounted for only 12 % of total clearance. Co-infusion of CCN-2 with receptor-associated protein (RAP), an antagonist of LDL-receptor family proteins, showed that RAP prolonged CCN-2 half-life and completely prevented CCN-2 internalization by hepatocytes. This suggests that hepatic uptake of CCN-2 is mediated by a RAP-sensitive mechanism most likely involving LRP1, a member of the LDL-receptor family involved in hepatic clearance of various plasma proteins. Surface plasmon resonance binding studies confirmed that CCN-2 is an LRP1 ligand. Co-infusion of CCN-2 with an excess of the heparan sulphate-binding protamine lowered the large initial distribution volume of CCN-2 by 88 % and reduced interstitial staining of CCN-2, suggesting binding of CCN-2 to heparan sulphate proteoglycans (HSPGs). Protamine did not affect clearance rate, indicating that RAP-sensitive clearance of CCN-2 is HSPG independent. In conclusion, unlike its amino-terminal fragment which is cleared by the kidney, fu

Small Aircraft RF Interference Path Loss Measurements

Interference to aircraft radio receivers is an increasing concern as more portable electronic devices are allowed onboard. Interference signals are attenuated as they propagate from inside the cabin to aircraft radio antennas mounted on the outside of the aircraft. The attenuation level is referred to as the interference path loss (IPL) value. Significant published IPL data exists for transport and regional category airplanes. This report fills a void by providing data for small business/corporate and general aviation aircraft. In this effort, IPL measurements are performed on ten small aircraft of different designs and manufacturers. Multiple radio systems are addressed. Along with the typical worst-case coupling values, statistical distributions are also reported that could lead to more meaningful interference risk assessment