Grid-enabled Workflows for Industrial Product Design

This paper presents a generic approach for developing and using Grid-based workflow technology for enabling cross-organizational engineering applications. Using industrial product design examples from the automotive and aerospace industries we highlight the main requirements and challenges addressed by our approach and describe how it can be used for enabling interoperability between heterogeneous workflow engines

Grapevine virus A and grapevine virus D are serologically distantly related

Grapevine trichovirus A (GVA), B (GVB), and D (GVD) are not serologically related as ascertained by ELISA and IEM tests using polyclonal antisera A study to investigate in detail their serological relationships was carried out with a larger number of reagents, including monoclonal antibodies (MAbs), and serological techniques (ELISA, IEM, tissue blot, Western blot). The results show that (i) polyclonal antisera to GVA, GVB and GVD cross-reacted in Western blot with all antigens; (ii) one out of 4 MAbs to GVA (MAb PA3.B9) reacted in ELISA, Western blot and tissue blot with the homologous virus and GVD but not with GVB. It is concluded that GVA, GVB and GVD are serologically distantly related and that the single antigenic determinant common to GVA and GVD is likely to be a cryptotope

Compressive sensing adaptation for polynomial chaos expansions

Basis adaptation in Homogeneous Chaos spaces rely on a suitable rotation of the underlying Gaussian germ. Several rotations have been proposed in the literature resulting in adaptations with different convergence properties. In this paper we present a new adaptation mechanism that builds on compressive sensing algorithms, resulting in a reduced polynomial chaos approximation with optimal sparsity. The developed adaptation algorithm consists of a two-step optimization procedure that computes the optimal coefficients and the input projection matrix of a low dimensional chaos expansion with respect to an optimally rotated basis. We demonstrate the attractive features of our algorithm through several numerical examples including the application on Large-Eddy Simulation (LES) calculations of turbulent combustion in a HIFiRE scramjet engine.Comment: Submitted to Journal of Computational Physic

Synchronous Disease Kinetics in a Murine Model for Enterohemorrhagic E. coli Infection Using Food-Borne Inoculation

Upon colonization of the intestinal epithelium, the attaching and effacing (AE) pathogen Enterohemorrhagic Escherichia coli (EHEC) effaces microvilli and forms pedestal-like structures beneath the adherent bacterium. The production of one of its virulence factors, the phage-encoded Shiga toxin (Stx) results in systemic disease, including the development of renal failure. Although EHEC does not productively infect conventional mice, EHEC infection can be modeled in mice utilizing a derivative of the natural murine AE pathogen Citrobacter rodentium(CR). Gavage of mice with CR(ΦStx2dact), a C. rodentium lysogenized by a phage encoding an Stx variant with high potency in mice, features AE lesion formation on intestinal epithelium and Stx-mediated systemic disease, including renal damage. This model is somewhat limited by mouse-to-mouse variation in the course of disease, with the time to severe morbidity (and required euthanasia) varying by as many as 5 days, a feature that limits pathological analysis at defined stages of disease. In the current study, we altered and optimized the preparation, dose, and mode of delivery of CR(ΦStx2dact), using food-borne route of infection to generate highly synchronous disease model. We found that food-borne inoculation of as few as 3 x 104 CR(ΦStx2dact) resulted in productive colonization and severe systemic disease. Upon inoculation of 1 x 108 bacteria, the majority of infected animals suffered weight loss beginning 5 days post-infection and all required euthanasia on day 6 or 7. This enhanced murine model for EHEC infection should facilitate characterization of the pathology associated with specific phases of Stx-mediated disease

Equation-Free Dynamic Renormalization: Self-Similarity in Multidimensional Particle System Dynamics

We present an equation-free dynamic renormalization approach to the computational study of coarse-grained, self-similar dynamic behavior in multidimensional particle systems. The approach is aimed at problems for which evolution equations for coarse-scale observables (e.g. particle density) are not explicitly available. Our illustrative example involves Brownian particles in a 2D Couette flow; marginal and conditional Inverse Cumulative Distribution Functions (ICDFs) constitute the macroscopic observables of the evolving particle distributions.Comment: 7 pages, 5 figure

Role of Sestrin 1 in Women with Polycystic Ovary Syndrome

Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disorder that affects at least 10% of women of reproductive age. Cystic ovaries, low or absent ovulation, and high circulating testosterone levels are the the group of symptoms that define polycystic ovary syndrome (PCOS).The aim of study: To investigate whether sestrin 1 can serve a prognostic indicator in polycystic ovary syndrome. The study included 100 women  with age ranged between (18 – 44) years involving 70 PCOS patients and 30 apparently healthy women as a control in reproductive age, at the reproductive fertility consultant of gynecological and obstetric teaching hospital, Kerbala health directorate Iraq and College of Applied Medical Sciences, University of Kerbala Iraq during the duration from October ., 2022 to April, 2023. The Rotterdam criteriOn-2003 was presumed to PCOS females. The result showed there were highly significant decreasing in woman with PCOS in contrast to woman without PCOS. According to BMI showed highly significant in the overweight at( P =0.001) whereas in the obesity at( P= 0.004) and normal weight at (P= 0.004). According to age showed highly significant in age group (18_23) while  in the age group( 24_29)at (P =0.000) and age group (30_35) at (P=0.006)  whereas age group (36_41) at( P=0.274) The conclusion indicated that reduced serum sestrin 1 levels may be has potential as a new PCOS diagnostic biomarker

Complex Contact-Based Dynamics of Microsphere Monolayers Revealed by Resonant Attenuation of Surface Acoustic Waves

Contact-based vibrations play an essential role in the dynamics of granular materials. Significant insights into vibrational granular dynamics have previously been obtained with reduced-dimensional systems containing macroscale particles. We study contact-based vibrations of a two-dimensional monolayer of micron-sized spheres on a solid substrate that forms a microscale granular crystal. Measurements of the resonant attenuation of laser-generated surface acoustic waves reveal three collective vibrational modes that involve displacements and rotations of the microspheres, as well as interparticle and particle-substrate interactions. To identify the modes, we tune the interparticle stiffness, which shifts the frequency of the horizontal-rotational resonances while leaving the vertical resonance unaffected. From the measured contact resonance frequencies we determine both particle-substrate and interparticle contact stiffnesses and find that the former is an order of magnitude larger than the latter. This study paves the way for investigating complex contact-based dynamics of microscale granular crystals and yields a new approach to studying micro- to nanoscale contact mechanics in multiparticle networks.National Science Foundation (U.S.) (Grant CMMI-1333858)United States. Army Research Office (Grant W911NF-15-1-0030)University of Washington. Royalty Research FoundationNational Science Foundation (U.S.) (Grant CHE-1111557

Enhancing WPA2-PSK four-way handshaking after re-authentication to deal with de-authentication followed by brute-force attack a novel re-authentication protocol

The nature of wireless network transmission and the emerging attacks are continuously creating or exploiting more vulnerabilities. Despite the fact that the security mechanisms and protocols are constantly upgraded and enhanced, the Small Office/Home Office (SOHO) environments that cannot afford a separate authentication system, and generally adopt the IEEE 802.11 Wi-Fi-Protected-Access-2/Pre-Shared-Key (WPA2-PSK) are still exposed to some attack categories such as de-authentication attacks that aim to push wireless client to re-authenticate to the Access Point (AP) and try to capture the keys exchanged during the handshake to compromise the network security. This kind of attack is impossible to detect or prevent in spite of having an Intrusion Detection and Prevention System (IDPS) installed on the client or on the AP, especially when the attack is not repetitive and is targeting only one client. This paper proposes a novel method which can mitigate and eliminate the risk of exposing the PSK to be captured during the re-authentication process by introducing a novel re-authentication protocol relying on an enhanced four-way handshake which does not require any hardware upgrade or heavy-weight cryptography affecting the network flexibility and performances

Properties of a new isolate of grapevine leafroll-associated virus 2

A new isolate of grapevine leafroll-associated virus 2 (GLRaV-2-H4) was recovered by mechanically inoculating herbaceous hosts with concentrated tissue extracts from a North American accession of Vitis rupestris. Contrary to the Semillon isolate of GLRaV-2, isolate H4 elicited necrotic local lesions in Nicotiana clevelandii and infected systemically N. occidentalis inducing very severe symptoms. The migration rate of dissociated capsid protein of GLRaV-2-H4 in SDS-PAGE differed slightly from that of GLRaV-2-Sem. The coat protein sequence of GLRaV-2-H4 differed by about 12 % at the nucleotide level from capsid proteins of the other two GLRaV-2 isolates that have been sequenced to date. No serological differences could be detected. Isolate H4 is a biological variant of GLRaV-2, which can be distinguished from other mechanically transmitted isolates of the same virus because of differences in type and reactions of the herbaceous host range and in molecular traits of the coat protein cistron