The minimal compound-complex sentence hierarchy

This article deals with the problem of the study of the subsystem of such type of the Multiple Compound-Complex Sentence as a minimal compound-complex sentence. The authors analyze the formal structure of the multiple compoundcomplex sentences and try to build up the structural models hierarchy of Minimal Compound-Complex SentenceyesBelgorod State Universit

Role of paramagnetic polyconjugated clusters in lignin antioxidant activity (in vitro)

Using physico-chemical methods (EPR, SEC, Py-GC/MS and UV/VIS spectroscopy) and wet chemical analysis, the characteristics of 6 hardwood lignins in terms of functionality, molecular weight and composition of lignin substructures were determined and considered together with the results of DPPH•, ABTS •+ and O2•- antioxidant assays with the aim to understand the relationships governing antioxidant properties of lignin. The strong positive linear correlation between lignin antioxidant capacity in the three assays used and the extent of conjugation of paramagnetic polyconjugated clusters in lignin macromolecules was found. The biological activity of the most active alkaline lignins was assessed by in vitro experiment with human blood.publishersversionPeer reviewe

Tracheobronchial injuries in blunt thoracic trauma

Catedra Chirurgie nr.1 ,,Nicolae Anestiadi’’, Catedra Chirurgie nr.4, USMF ,,Nicolae Testemiţanu’’, Secția Chirurgie Toracică, Departament Clinic Chirurgie IMU, Chișinău, Republica Moldova, Conferința stiințifică „Nicolae Anestiadi – nume etern al chirurgiei basarabene” consacrată centenarului de la nașterea profesorului Nicolae Anestiadi 26 august 2016Introducere. Majoritatea traumatismelor toracice severe (80%) sunt rezultatul politraumatismelor. Acestea reprezintă o entitate asociată unui risc sporit de morbiditate şi mortalitate de până la 15-30%. Leziunea traheobronşică, deşi rară ca incidenţă (1,5-3%), reprezintă un traumatism potenţial letal prin dezvoltarea complicațiilor precoce (insuficiență cardiorespiratorie acută) și tardive (sepsisul pulmonar), care sunt dependente direct de momentul diagnosticării. Scop. Aprecierea factorilor preoperatorii determinanți necesari stabilirii precoce a diagnosticului şi tacticii chirurgicale adecvate în timpi oportuni. Material şi metode. Trei politraumatizaţi (Cr,To,Lo) cu leziune predominantă toracică, vârsta medie – 23,67±2,73ani, raportul B:F/2:1, perioada de referință 2011-2015, cu ISS=38p(3), RTS=6,171(1) și 7,841(2), și TRISS – 20,3%(1), 6,2%(2). În 100% cazuri s-a efectuat hemoleucograma, radiografia toracelui, USG-FAST și FBS, TC doar într-un caz. Durata accident-intervenţie: până la 12h(2), 5zile(1) – transfer AVIASAN. Rezultate. Rx-toracic a evidenţiat: fracturi costale multiple bilaterale(2), unilaterale(1), hemopneumotorace bilateral(2), pneumotorace bilateral(1) și pneumomediastinum(2) cu suspecție de leziune bronşică într-un caz. S-a efectuat toracocenteză bilaterală (100%) și drenarea mediastinului anterior (1). TC a evidențiat fracturile costale, contuzia pulmonară și pneumotoracele persistent. Fluxul aerian continuu prin drenajele montate a impus necesitatea bronhoscopiei diagnostice (100%), care a confirmat leziunea bronșică (BPD – 2, BPS – 1). S-a efectuat toracotomie cu plastia bronhiei lezate(3) și lobectomie superioară dreaptă(1). Evoluția postoperatorie favorabilă, durata de spitalizare – 17,67±2,72zile. Toți au dezvoltat pneumonie, confirmată prin examenele imagistice și paraclinice. Concluzii. Instabilitatea hemodinamică și respiratorie acută, prezența fluxului de aer continuu posttoracocenteză și examenele imagistice impun suspecția leziunii traheobronșice și necesită confirmare prin fibrobronhoscopie. Ultima asigură diagnosticul cert şi determină tactica chirurgicală oportună în timpi efectivi.Introduction. The majority of severe thoracic trauma (80%) result from polytrauma. It is associated with a high risk of morbidity and mortality (15-30%). Despite the rare incidence of tracheobronchial injury (1,5- 3%), the lethality is high due to development of early complications (acute cardiorespiratory failure) and late complications (lung sepsis) that depend directly on the time of diagnosis. Purpose. Appreciation of determinant preoperative factors, necessary to establish on time an accurate diagnosis and surgical treatment. Material and methods. Three polytraumatized patients (Cr,Th,Loco) with predominant thoracic injury, mean age – 23,67±2,73 years, M:F ratio 2:1, hospitalized between 2011-2015. ISS=38p(3), RTS= 6,171(1) and 7,841(2), TRISS – 20,3%(1), 6,2%(2). In 100% of cases investigations include: chest x-ray, FAST and FBS, TC – one case. The time period injury-intervention: up to 12h(2), 5 days(1) – AVIASAN transfer. Results. Chest x-ray revealed: multiple bilateral rib fractures(2), unilateral(1), bilateral hemopneumothorax(2), bilateral pneumothorax(1) and pneumomediastinum(2) with suspicion of bronchial lesion in one case. Bilateral thoracocentesis was performed in all patients, anterior mediastinal drainage – in one. TC revealed rib fractures, lung contusion and persistent pneumothorax. The continuous airflow through drainages required diagnostic bronchoscopy(100%) that confirmed the bronchial lesion (RMB – 2, LMB – 1). Thoracotomy with the plasty of injured bronchus was performed in all patients, right upper lobectomy in 1. Postoperative evolution – favorable, hospitalization period – 17,67±2,72 days. All patients developed pneumonia, confirmed by imagistic examinations. Conclusions. Acute hemodynamic and respiratory instability, the presence of the continuous airflow through drainages and imagistic examinations lead to suspicion of tracheobronchial injury, which required a confirmation by fibrobronchoscopy, that guarantees the right diagnosis and surgical treatment on time

Parameters of strength fitness in athletes from various team sports

Objective of the study was to identify the parameters of strength fitness of athletes of different skill levels from various team sports and conduct a comparative analysis of the data obtaine

Efficiency of Using the Hydrogen Peroxide Vapor Generator “Fhileas 75” for Disinfection of the Air Ducts of separately Ventilated system for Infected Animal Housing

The aim of the work was to evaluate the efficiency of using the “Fhileas 75” hydrogen peroxide vapor generator for decontaminating the air ducts of the individually ventilated system, “Bio A.S.”, for housing of infected animals.Materials and methods. The hydrogen peroxide vapor generator “Fhileas 75” (France), a disinfectant manufactured by “FHILEASAFE” (7 % hydrogen peroxide solution and 0.15 % peracetic acid solution), separately ventilated system “Bio A.S.” (Germany) for the infected animal housing were applied in the work. Serratia marcescens 9 was used as test-culture.Results and discussion. The efficiency of using the hydrogen peroxide vapor generator “Fhileas 75” for decontamination of air ducts and internal surfaces of the rack of the individually ventilated system “Bio A.S.” on the test-culture S. marcescens 9 at 1·106 mc/ml concentration has been established (operation parameters of the individually ventilated system unit are as follows: air exchange rate – 60 changes per hour, air flow volume – 28 m3/hour, number of disinfection cycles – 5, disinfectant spraying time – 97 min, exposure time – 24 hours)

Enhancement of the Technology for Live Tularemia Vaccine Production

Objective of the study was to develop and test new biotechnological approaches for live tularemia vaccine production.Materials and methods: Francisella tularensis 15 NIIEG strain was used as producer-strain; Francisella tularensis 503 strain – as test infecting one. Producer strain was cultivated on solid and liquid nutrient media. Tangential ultrafiltration was performed with the help of microfiltration module “Viva-flow”. Lyophilization was conducted using drying installation – Free Zone 2.5 L.Results and discussion: Application of the designed liquid nutrient medium on the basis of enzymatic fibrin hydrolysate and submerged cultivation of the producer-strain has allowed for a significant biomass yield increment. At the stage of tularemia microbe culture concentration via microfiltration through filtering membranes with pore size of 0.2 μm, in the mode of tangential liquid flow, increased has been the content of microbe cells; the nutrient media residues – removed. Comparative analysis of the obtained in accordance with experimental technique laboratory series of the vaccine and commercial preparation of live tularemia vaccine has demonstrated their conformity with the specific normative properties. It is established that application of modified liquid nutrient medium, submerged cultivation conditions, methods of biomass concentration and separation has no negative influence on the main properties of live tularemia vaccine and will provide for considerable produce-ability increase in the future

Effectiveness of the “Sterius 60” SHF Radiation Installation for Disinfection of Objects Contaminated with PBA of Groups I–IV, when Working with Infected Biomodels

The aim was to evaluate the effectiveness of using the “Sterius 60” microwave disinfection system (Russia) for decontamination of objects infected with PBA of groups I–IV emerging as a result of working with infected laboratory animals.Materials and methods. Effectiveness verification of disinfection of biological waste generated as a result of the life of laboratory animals by SHF radiation was carried out in the microwave system “Sterius 60”, recommended by the manufacturer for disinfection of epidemiologically hazardous and extremely dangerous medical waste, including biological ones (classes B and C), by volumetric SHF heating. Carcasses of uninfected laboratory animals (white mice, Guinea pigs, suckling rabbits), granulated feed and bedding material (wood shavings), which are objects directly in contact with biomodels, were used as vivarium waste to be decontaminated. The following microorganisms were utilized as model test ones: Bacillus subtilus VKM B-911, Bacillus stearothermophilus VKM B-718, Bacillus licheniformis G VKM B-1711-D, Alcaligenes faecalis 415, Yersinia pestis EV, Bacillus anthracis STI. Laboratory utensils (plastic Petri dishes, porcelain mortars and pestles) were used as a mock-up chamber filler for model test microorganisms.Results and discussion. As a result of the study, data were obtained indicating that the microwave system for disinfection of medical waste “Sterius 60” is ineffective for decontamination of biological waste in laboratories working with biomodels infected with PBA of groups I–II. The established standard mode of disinfection of this system was effective only for non-spore forms of microorganisms, pathogenicity groups III–IV. Therefore, in our opinion, it is advisable to use it for decontamination of laboratory utensils infected with PBA of groups III–IV, directly at sites of waste generation

Construction and Investigation of the Variants of the Vaccine Strain <I>Francisella tularensis</I> Lacking <I>iglC </I>Genes. Communication 2

for BALB/c mice by an order while retaining protective properties. Strain Francisella tularensis 15 lacking two copies of the iglC gene cannot replicate in mice organism, induces weak humoral response, is fully avirulent, and has decreased protective activity. Treatment of mice with sera obtained from the animals immunized on day 49 post immunization both with the stock F. tularensis 15 strain and its variants with one or two inactivated iglC genes has provided for 100 % protection from challenge with 200 DCL of F. tularensis 15 strain. However in case of BALB/c mice exposure to virulent F. tularensis 503 and F. tularensis Schu strains (50 DCL and 25 DSL, respectively), treated with immune sera 24 hours before, registered has been only mean lifetime increase