28 research outputs found
Increased Anion Channel Activity Is an Unavoidable Event in Ozone-Induced Programmed Cell Death
Ozone is a major secondary air pollutant often reaching high concentrations
in urban areas under strong daylight, high temperature and stagnant
high-pressure systems. Ozone in the troposphere is a pollutant that is
harmful to the plant. generation by salicylic and abscisic acids.
Anion channel activation was also shown to promote the accumulation of
transcripts encoding vacuolar processing enzymes, a family of proteases
previously reported to contribute to the disruption of vacuole integrity
observed during programmed cell death.-induced
programmed cell death. Because ion channels and more specifically anion
channels assume a crucial position in cells, an understanding about the
underlying role(s) for ion channels in the signalling pathway leading to
programmed cell death is a subject that warrants future investigation
Classification et prise en charge thérapeutique des gammapathies monoclonales de signification rénale
International audienc
Ethylene stimulation of latex yield depends on the expression of a sucrose transporter (HbSUT1B) in rubber tree (Hevea brasiliensis)
An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells
International audienceThe pathogenicity of various Streptomyces scabies isolates involved in potato scab disease was correlated with the production of thaxtomin A. Since calcium is known as an essential second messenger associated with pathogen-induced plant responses and cell death, it was investigated whether thaxtomin A could induce a Ca 2+ influx related to cell death and to other putative plant responses using Arabidopsis thaliana suspension cells, which is a convenient model to study plant–microbe interactions. A. thaliana cells were treated with micromolar concentrations of thaxto-min A. Cell death was quantified and ion flux variations were analysed from electrophysiological measurements with the apoaequorin Ca 2+ reporter protein and by external pH measurement. Involvement of anion and calcium channels in signal transduction leading to programmed cell death was determined by using specific inhibitors. These data suggest that this toxin induces a rapid Ca 2+ influx and cell death in A. thaliana cell suspensions. Moreover, these data provide strong evidence that the Ca 2+ influx induced by thaxtomin A is necessary to achieve this cell death and is a prerequisite to early thaxtomin A-induced responses: anion current increase, alkalization of the external medium, and the expression of PAL1 coding for a key enzyme of the phenylpropanoid pathway