Contribution of some immunological and metabolic factors to formation of piglets’ post-vaccination immunity

The role and responsibility of natural resistance factors, protein and lipid metabolism in the formation of piglets post-vaccination immunity against circovirus is researched. Blood was taken for tests before and on the 15th, 40th and 70th day after the vaccination. The sampled blood was analyzed to determine immunological and biochemical parameters. It was revealed that before vaccination, 31.46% of the studied samples have a positive reaction in ELISA; their number increases to 67.80–71.16% on the 40th and 70th days after vaccination.In the blood of piglets, especially on the 40th and 70th day after the vaccination, the total count of leukocytes, monocytes and lymphocytes increases by 1.21; 2.28 times and 1.48 times, but neutrophils reduced by 1.74 times along with the phagocytic properties activation. The anabolic directivity of protein metabolism is defined by the synthesis of globulin proteins. At the same time albumin-synthesizing activity in a liver decreased and “cytolysis reaction” of hepatocytes was detected. In the lipid profile of piglets’ blood, the content of LDL‑cholesterol increased by 1.44 times, while that of triglycerides decreased by 2.64 times. X‑ray spectral analysis revealed the correlation between the formation of post-vaccination immunity and two factors: the factor of the principal component (PC) 1, which is predominantly associated with indicators of natural resistance, and PC2, which is associated with metabolism indicators. The research results show that in order to increase the efficiency of formation of post-vaccination immunity in piglets, it is necessary to combine vaccination with hepatoprotective drugs

Microbial associations for pneumonia causative agents and level of their resistance to antimicrobial drugs during a new coronavirus infection pandemic

Introduction. Bacterial coinfection and secondary bacterial infection are considered critical risk factors for the severity and mortality of SARS-CoV-2-caused pneumonia. The aim of the study was to analyze a pattern of microbial associations between K. pneumoniae and A. baumannii isolated from the lower respiratory tract discharge and sectional material (lung tissue) of patients diagnosed with pneumonia, and to compare resistance level in monoculture and associations during new coronavirus infection pandemic. Materials and methods. A bacteriological study of 2689 sputum and bronchial washing samples from patients at infectious diseases hospitals, and 1411 lung pathological material samples was carried out. Bacterial isolates were identified by mass spectrometry. Antibiotic sensitivity for isolates was determined by the disk diffusion method. Genetic determinants of resistance to beta-lactam antibiotics were detected by PCR. Statistical data processing was performed using SPSS version 22 software. Results. K. pneumoniae and A. baumannii isolates were predominantly found in two- and three-pathogen associations. It was established that the resistance level of K. pneumoniae isolates in association with A. baumannii is significantly higher compared to that in monoculture for all antimicrobial drugs studied. At the same time, K. pneumoniae in combination with Candida spp. vs monoculture showed significantly lower level of resistance to ciprofloxacin, amikacin, cefotaxime, ceftazidime and amoxicillin/clavulanic acid. K. pneumoniae isolates carried resistance determinants to extended-spectrum beta-lactamases: OXA-48 — (22.5%), OXA-51 — (5.6%), OXA-23 — (4.2%), KPC — 70.9%, NDM — 7%. Of these, 14.1% of strains had the ability to co-produce serine carbapenemases OXA-48 and KPC. Sputum and lung tissue A. baumannii isolates exhibited extremely high multiple resistance regardless of their associations with other microorganisms. Microbiome species similarity in the lower respiratory tract and lung tissue discharge was revealed. The proportion of lung tissue vs sputum resistant strains of K. pneumoniae and A. baumannii was significantly higher. Conclusion. The detection of of multiple drug resistant K. pneumoniae and A. baumannii isolates as well as their associations may indicate aggravated pneumonia severity

ESCHERICHIA COLI phenotypic characteristics and antagonistic activity in opisthorchiasis invasion

Opisthorchis felineus invasion in human causes inflammatory and dyskinetic disorders of the gastrointestinal tract accompanied by altered phenotypic characteristics in colon microbiota. The aim of research — study an impact of the Escherichia coli isolate phenotypic characteristics on Klebsiella spp. bacteria, isolated from colonic contents of patients with diagnosed opisthorchiasis as well as E. coli antagonistic activity. Materials and methods. The phenotypic properties of 54 E. coli isolates and 8 genus Klebsiella isolates obtained from colonic contents of patients with diagnosed opisthorchiasis were assessed. Identification of isolates and analysis of proteomic profiles were performed using Maldi BioTyper 3.0 software. 204 co-cultivation datasets were analyzed investigating antagonistic activity of E. coli isolates with varying properties on Klebsiella spp. E. coli and Klebsiella spp. isolates were examined by whole genome sequencing. Results. E. coli bacteria with typical phenotypic characteristics showed significantly more prominent antagonistic activity against Klebsiella spp. A significantly higher level of antagonistic activity against K. oxytoca bacteria vs K. pneumoniae strains. The proteomic bacterial strain profiles were divided into clusters depending on the level of antagonistic activity. E. coli molecular serotyping for O- and H-antigens revealed the genes of enterotoxigenic, enteroinvasive and extraintestinal pathogens in 60.0% of cases. Strains with the highest antagonistic activity index, which are carriers of the genes typical to enterotoxigenic E. coli sequence serotypes O6:H1 and O6:H5, were identified. The genome of such strains consisted of the largest number of virulence gene complexes: adhesins, invasins, toxins, bacteriocins. Multilocus sequence typing and sequence serotyping of E. coli and K. pneumoniae strains established their heterogeneity; K. oxytoca isolates were identified as ST242 and ST176. All strains were characterized by homology of antibiotic resistance markers (oqxA, oqxB, fosA) and a variety of beta-lactam resistance gene variants. Conclusion. It was found that E. coli isolates with typical phenotypic characteristics and carriers of virulence gene complexes exhibited significantly more pronounced antagonistic activity against Klebsiella spp. isolated from colonic contents of patients with diagnosed opisthorchiasis

Pathological features of the lungs and liver of piglets under conditions of constant vaccination of livestock against circovirus infection

The pathogenicity of PCV 2 in the body of vaccinated piglets was studied based on the results of pathomorphological changes in the lungs and liver of animals. The work was carried out on commercial piglets vaccinated with the vaccine Ingelvak CircoFLEX (Germany) against circovirus. The work used clinical, zootechnical, enzyme immunoassay and pathomorphological research methods. It has been established that under the conditions of ongoing vaccination of piglets against PCV2, 30.3% of piglets still do not have virus-neutralizing antibodies. The main reason for the culling of animals are circovirus diseases that have respiratory clinical signs, as well as signs of multisystem wasting syndrome, determining the safety of the livestock at the level of 68.05%, the average live weight of 1 head at the moment of its transfer for fattening is 40.44±0.78 kg, and the average daily gain in live weight is 346.00±9.18 g. At autopsy, sick piglets reveal an increase in the lungs and liver, and the signs of inflammation in them, as a result of circulatory disorders, damage to the lymphoid tissue, the development of dystrophic and necrotic changes. The results of the research suggest that in order to increase the efficiency of the formation of post-vaccination immunity, specific medical preparations can be used to stimulate the immune response of the body, as well as to enhance the resistance of the lymphoid tissue of the lungs and liver in animals.The pathogenicity of PCV 2 in the body of vaccinated piglets was studied based on the results of pathomorphological changes in the lungs and liver of animals. The work was carried out on commercial piglets vaccinated with the vaccine Ingelvak CircoFLEX (Germany) against circovirus. The work used clinical, zootechnical, enzyme immunoassay and pathomorphological research methods. It has been established that under the conditions of ongoing vaccination of piglets against PCV2, 30.3% of piglets still do not have virus-neutralizing antibodies. The main reason for the culling of animals are circovirus diseases that have respiratory clinical signs, as well as signs of multisystem wasting syndrome, determining the safety of the livestock at the level of 68.05%, the average live weight of 1 head at the moment of its transfer for fattening is 40.44±0.78 kg, and the average daily gain in live weight is 346.00±9.18 g. At autopsy, sick piglets reveal an increase in the lungs and liver, and the signs of inflammation in them, as a result of circulatory disorders, damage to the lymphoid tissue, the development of dystrophic and necrotic changes. The results of the research suggest that in order to increase the efficiency of the formation of post-vaccination immunity, specific medical preparations can be used to stimulate the immune response of the body, as well as to enhance the resistance of the lymphoid tissue of the lungs and liver in animals

Intoxication syndrome in patients with pulmonary tuberculosis in relation to the system glutathione transferase gene polymorphism

In 178 patients with different clinical forms of pulmonary tuberculosis carried out pharmacogenetic testing to determine gene polymorphism glutathione S-transferase system (GSTM1, GSTT1). Estimated the severity and duration of clinical manifestations of the syndrome of intoxication, depending on the gene polymorphism of glutathione S-transferase system (GSTM1, GSTT1). It was found that the combination of “zero” to develop genotypes GSTM1 and GSTT1 enzyme is a predictor of the severity and duration of symptoms of intoxication persisting

Listen to genes : dealing with microarray data in the frequency domain

Background: We present a novel and systematic approach to analyze temporal microarray data. The approach includes normalization, clustering and network analysis of genes. Methodology: Genes are normalized using an error model based uniform normalization method aimed at identifying and estimating the sources of variations. The model minimizes the correlation among error terms across replicates. The normalized gene expressions are then clustered in terms of their power spectrum density. The method of complex Granger causality is introduced to reveal interactions between sets of genes. Complex Granger causality along with partial Granger causality is applied in both time and frequency domains to selected as well as all the genes to reveal the interesting networks of interactions. The approach is successfully applied to Arabidopsis leaf microarray data generated from 31,000 genes observed over 22 time points over 22 days. Three circuits: a circadian gene circuit, an ethylene circuit and a new global circuit showing a hierarchical structure to determine the initiators of leaf senescence are analyzed in detail. Conclusions: We use a totally data-driven approach to form biological hypothesis. Clustering using the power-spectrum analysis helps us identify genes of potential interest. Their dynamics can be captured accurately in the time and frequency domain using the methods of complex and partial Granger causality. With the rise in availability of temporal microarray data, such methods can be useful tools in uncovering the hidden biological interactions. We show our method in a step by step manner with help of toy models as well as a real biological dataset. We also analyse three distinct gene circuits of potential interest to Arabidopsis researchers

Rare earth elements materials production from apatite ores

The paper deals with the study of processing apatite ores with nitric acid and extraction of the rare earth elements. The rare earth elements can be successfully separated and recovered by extraction from the nitrate- phosphate solution, being an tributyl phosphate as extraction agent. The developed scheme of the processing apatite concentrate provides obtaining rare earth concentrates with high qualitative characteristics

Клінічні симптоми ушкодження латеральної поверхні гомілкового суглобу дійних корів різного фенотипу в умовах промислового молочного виробництва

The results of the influence of the conditions of keeping Ukrainian red dairy cows on the manifestation of clinical symptoms of damage to the lateral surface of the tibia and the level of reproduction are presented. The study was conducted on the basis of an industrial dairy complex (600 dairy cows with an average productivity of 6050 kg of milk per lactation) as part of agricultural associations in Odessa region. The objects of the study were cows during lactation, which were kept loose in sections with rest boxes (where the floor was a special rubber mat with a thickness of 30 mm). Evaluation (in points) was performed on the clinical manifestation of symptoms of damage to the skin epithelium of the lateral surface of the leg, which was injured by the rubber coating of the stall compared to the level of reproduction (service period, days/%) and the severity of skin pigmentation of the body surface (selection melanism). Clinical, zootechnical, biometric methods were comprehensively applied to realize the purpose of research. Comparison of data (n = 462) visual-clinical assessment of the condition of the skin of the lateral surface of the leg, as an indicator of the adaptability of lactating cows to keep in sections with rubber flooring and the area of pigmented hair of animals of different phenotypes by coat color, showed that cows typical pigmentation, characteristic of red dairy breed (red hair occupied more than 80 % of the body surface), wound lesions of the lower leg are much less common, namely: in comparison with red-spotted animals by 28.41–15.98 %, with mostly white – by 39.03–20.23 %, respectively. Due to the negative impact of open wounds of the skin of the leg on the immunity of lactating cows, it was found that increasing the number of animals with damaged epithelium as a gateway to infection, provokes an increase in the service period in cows with predominantly white or mottled color to 140.97 and 141.07 days, which is higher by 6.74 and 6.84 days, respectively, compared with red animals (P < 0.05).Надані результати впливу умов утримання  корів української червоної молочної породи на прояв клінічних симптомів ушкодження латеральної поверхні гомілкового суглоба та рівень репродукції. Дослідження проведено на базі промислового молочного комплексу (600 дійних корів із середньою продуктивністю 6252 кг молока за лактацію) у складі сільськогосподарських об’єднань  Одеської області. Об’єктами дослідження були корови впродовж перебігу лактації, які безприв’язно утримувались у секціях з  боксами для відпочинку (де підлогою були спеціальні гумові килимки з товщиною 30 мм). Оцінку (в балах) проводили за клінічним проявом симптомів ушкодження шкірного епітелію латеральної поверхні гомілки, яка травмувалась гумовим покриттям стійла, порівняно з рівнем відтворення (сервіс-період, днів; між отельний період МОП, днів; кількість тільних самиць у групі, гол./%) та вираженістю пігментації шкірного покриву поверхні тіла (селекційним меланізмом). Для реалізації мети дослідження комплексно були застосовані клінічні, зоотехнічні, біометричні методи. Зіставлення даних (n = 462) візуально-клінічної оцінки стану шкіри латеральної поверхні гомілки як індикатору адаптаційної здатності лактуючих корів до утримання в секціях з гумовим покриттям підлоги та площі пігментованого волосяного покриву тіла тварин різного фенотипу за забарвленням шерсті свідчили, що у корів з типовою пігментацією, характерною для червоної молочної породи (червона шерсть займала понад 80 % поверхні тіла), ранові ураження гомілки трапляються значно рідше, а саме: порівняно з червоно-рябими тваринами на 28,41–15,98 %, з переважно білими – на 39,03–20,23 % відповідно. Зважаючи на негативний вплив відкритих ран  шкіри гомілки на імунітет лактуючих корів, встановлено, що збільшення числа тварин з  пошкодженим епітелієм як воротами інфекції провокує ріст сервіс-періоду у корів з переважно білою або рябою мастю до 140,97 і 141,07 днів, що відповідно більше на 6,74 і 6,84 днів порівняно з тваринами червоної масті (P < 0,05)

The oxidative status in patients with chronic kidney disease

An excess of free radicals accompanies the development of renal pathologies and causes numerous concomitant complications and syndromes. The most common of these are cardiometabolic syndromes in patients with chronic kidney disease. Therefore, the purpose of the study was to determine the activity of paraoxonase-1 and myeloperoxidase, which are associated with indicators of high-density lipoproteins content and oxidative stress in the blood of patients with the chronic stage of kidney disease. The activity of the enzymes, thiobarbiturate-active products concentration and transferrin, ceruloplasmin, thiol compounds content were determined in the blood of patients with chronic kidney disease. The oxidative status was shown to be changed. Thus, myeloperoxidase activity, the content of oxidized proteins and the concentration of thiobarbiturate-positive components were increased, while the activity of the antioxidant enzyme paraoxonase-1, the content of transferrin, ceruloplasmin and thiol compounds were decreased. The ratio of myeloperoxidase/paraoxonase-1 activities was progressively increased up to 9-fold, indicating the presence of cardiovascular complications in patients. The data obtained allowed to extend the range of indicators for monitoring the development of cardiometabolic disorders in the progression of chronic kidney disease