Shifting gender relations at Khok Phanom Di, Thailand: Isotopic evidence from the skeletons

The values for isotopes of strontium, carbon, and oxygen in human tooth enamel from the prehistoric site of Khok Phanom Di (ca. 2100–1500 BC) in Thailand shed light on human mobility and marital residence during a crucial period of subsistence change. Khok Phanom Di was a sedentary coastal community that apparently relied on hunting, gathering, and fishing in the midst of a transition to rice agriculture in the interior. The results of the isotope analyses indicate female immigration and then a marked shift to local strontium isotope signatures among females accompanied by a clear increase in the prestige of female burials. A possible explanation is a shift in the pattern of exogamy with a concomitant change in gender relations. Observation of a very similar transition at Ban Chiang, in northeastern Thailand, suggests the possibility of a regionwide social transition. In the case of Khok Phanom Di, the increasing role of females in producing high-quality ceramic vessels may have contributed to the change

Detection of Mycobacterium leprae DNA from Archaeological Skeletal Remains in Japan Using Whole Genome Amplification and Polymerase Chain Reaction

BACKGROUND: Identification of pathogen DNA from archaeological human remains is a powerful tool in demonstrating that the infectious disease existed in the past. However, it is very difficult to detect trace amounts of DNA remnants attached to the human skeleton, especially from those buried in a humid atmosphere with a relatively high environmental temperature such as in Asia. METHODOLOGY/PRINCIPAL FINDINGS: Here we demonstrate Mycobacterium leprae DNA from archaeological skeletal remains in Japan by polymerase chain reaction, DNA sequencing and single nucleotide polymorphism (SNP) analysis. In addition, we have established a highly sensitive method of detecting DNA using a combination of whole genome amplification and polymerase chain reaction, or WGA-PCR, which provides superior sensitivity and specificity in detecting DNA from trace amounts of skeletal materials. CONCLUSION/SIGNIFICANCE: We have detected M. leprae DNA in archaeological skeletal remains for the first time in the Far East. Its SNP genotype corresponded to type 1; the first detected case worldwide of ancient M. leprae DNA. We also developed a highly sensitive method to detect ancient DNA by utilizing whole genome amplification

Spinal infection: state of the art and management algorithm

Spinal infection is a rare pathology although a concerning rising incidence has been observed in recent years. This increase might reflect a progressively more susceptible population but also the availability of increased diagnostic accuracy. Yet, even with improved diagnosis tools and procedures, the delay in diagnosis remains an important issue. This review aims to highlight the importance of a methodological attitude towards accurate and prompt diagnosis using an algorithm to aid on spinal infection management. METHODS: Appropriate literature on spinal infection was selected using databases from the US National Library of Medicine and the National Institutes of Health. RESULTS: Literature reveals that histopathological analysis of infected tissues is a paramount for diagnosis and must be performed routinely. Antibiotic therapy is transversal to both conservative and surgical approaches and must be initiated after etiological diagnosis. Indications for surgical treatment include neurological deficits or sepsis, spine instability and/or deformity, presence of epidural abscess and upon failure of conservative treatment. CONCLUSIONS: A methodological assessment could lead to diagnosis effectiveness of spinal infection. Towards this, we present a management algorithm based on literature findings

Review of nanomaterials in dentistry: interactions with the oral microenvironment, clinical applications, hazards, and benefits.

Interest in the use of engineered nanomaterials (ENMs) as either nanomedicines or dental materials/devices in clinical dentistry is growing. This review aims to detail the ultrafine structure, chemical composition, and reactivity of dental tissues in the context of interactions with ENMs, including the saliva, pellicle layer, and oral biofilm; then describes the applications of ENMs in dentistry in context with beneficial clinical outcomes versus potential risks. The flow rate and quality of saliva are likely to influence the behavior of ENMs in the oral cavity, but how the protein corona formed on the ENMs will alter bioavailability, or interact with the structure and proteins of the pellicle layer, as well as microbes in the biofilm, remains unclear. The tooth enamel is a dense crystalline structure that is likely to act as a barrier to ENM penetration, but underlying dentinal tubules are not. Consequently, ENMs may be used to strengthen dentine or regenerate pulp tissue. ENMs have dental applications as antibacterials for infection control, as nanofillers to improve the mechanical and bioactive properties of restoration materials, and as novel coatings on dental implants. Dentifrices and some related personal care products are already available for oral health applications. Overall, the clinical benefits generally outweigh the hazards of using ENMs in the oral cavity, and the latter should not prevent the responsible innovation of nanotechnology in dentistry. However, the clinical safety regulations for dental materials have not been specifically updated for ENMs, and some guidance on occupational health for practitioners is also needed. Knowledge gaps for future research include the formation of protein corona in the oral cavity, ENM diffusion through clinically relevant biofilms, and mechanistic investigations on how ENMs strengthen the tooth structure

Adult fracture patterns in prehistoric Thailand: a biocultural interpretation

The prevalence and distribution patterns of trauma in samples of human skeletal remains can reflect the risks to which the community was exposed in daily activities or as a result of interpersonal violence. This paper describes the patterns of non-vertebral fractures in skeletal samples from four prehistoric Thai sites in terms of long bone fracture rates and individual prevalence rates. The sites range in date from c. 2000 BC (Neolithic) to 400 BC (late Bronze Age) and in environment from coastal estuarine to seasonally dry upland plains. These differences in the natural and cultural environment provided a basis for comparison among the samples representing nearly 300 adult individuals. The types of fractures ranged from simple to severe but most had healed successfully with few limiting complications. The small bones of the hands and feet as well as clavicle and forearm bones were most frequently fractured among all samples. Overall there was an increase in the major long bone fracture rates from the Neolithic (0.3%) to the Bronze Age (3.0%) that may reflect a change in subsistence activities such as land clearance for the intensification of rice agriculture. The prevalence of ulnar fractures is particularly high in the Bronze Age and the analysis of their possible cause, combined with evidence for craniofacial fractures, is suggestive of the presence of interpersonal violence in a small number of individuals