Simulation of old open clusters for UVIT on ASTROSAT

Ultra Violet Imaging Telescope (UVIT) is one of the payloads on the first Indian multi wavelength satellite ASTROSAT expected to be launched by Indian Space Research Organisation (ISRO) in the year 2015. We have performed simulations of UV studies of old open clusters for the UVIT. The colour magnitude diagrams (CMDs) and spatial appearances have been created using 10 filters of FUV channel (130 - 180 nm) and NUV channel (200 - 300nm) available for observations on the UVIT, for three old open clusters M67, NGC 188 and NGC 6791. The CMDs are simulated for different filter combinations, and they are used to identify the loci of various evolutionary sequences, white dwarfs, blue stragglers, red giants, sub giants, turn off stars and the main sequence of the clusters. The present work helps in identifying the potential area of study in the case of three old open clusters, by considering the availability of filters and the detection limits of the instrument. We also recommend filter combinations, which can be used to detect and study the above mentioned evolutionary stages. The simulations and the results presented here are essential for the optimal use of the UVIT for studies of old open clusters.Comment: Accepted for publication in RAA; 25 pages, 19 figures, 1 tabl

Genome sequence of a novel alloherpesvirus isolated from glass catfish (Kryptopterus bicirrhis)

The 149,343-bp genome of silurid herpesvirus 1, which was isolated in Thailand from glass catfish, was sequenced. The genome was most closely related to that of ictalurid herpesvirus 2, which infects black bullhead catfish. To our knowledge, this was the first silurid catfish alloherpesvirus genome to be sequenced

On the determination of age and mass functions of stars in young open star clusters from the analysis of their luminosity functions

Based on the CCD observations of remote young open clusters NGC 2383, NGC 2384, NGC 4103, NGC 4755, NGC 7510 and Hogg 15, we constructed their observed luminosity functions (LFs). The observed LFs are corrected for field star contamination determined with the help of galactic star count model. In the case of Hogg 15 and NGC 2383 we also considered the additional contamination from neighbouring clusters NGC 4609 and NGC 2384 respectively. These corrections provided the realistic pattern of cluster LF in the vicinity of the MS turn on point and at fainter magnitudes, revealed the so called H-feature arising due to transition of the Pre-MS phase to MS, which is dependent on the cluster age. The theoretical LFs were constructed representing a cluster population model with continuous star formation for a short time scale and a power law Initial Mass Function (IMF) and these were fitted to the observed LF. As a result we are able to determine for each cluster a set of parameters, describing cluster population (the age, duration of star formation, IMF slope and percentage of field star contamination). It was found that in spite of the non-monotonic behaviour of observed LFs, cluster IMFs can be described as the power law functions with slopes similar to Salpeter's value. The present MS turn on cluster ages are several times lower than those derived from the fitting of theoretical isochrones to the turn off region of the upper Main Sequences.Comment: 17 pages, 5 figures, To appear in MNRA

Optical/IR studies of Be stars in NGC 6834 with emphasis on two specific stars

We present optical and infrared photometric and spectroscopic studies of two Be stars in the 70--80-Myr-old open cluster NGC 6834. NGC 6834(1) has been reported as a binary from speckle interferometric studies whereas NGC 6834(2) may possibly be a gamma Cas-like variable. Infrared photometry and spectroscopy from the United Kingdom Infrared Telescope (UKIRT), and optical data from various facilities are combined with archival data to understand the nature of these candidates. High signal-to-noise near-IR spectra obtained from UKIRT have enabled us to study the optical depth effects in the hydrogen emission lines of these stars. We have explored the spectral classification scheme based on the intensity of emission lines in the $H$ and $K$ bands and contrasted it with the conventional classification based on the intensity of hydrogen and helium absorption lines. This work also presents hitherto unavailable UBV CCD photometry of NGC 6834, from which the evolutionary state of the Be stars is identified.Comment: Published in Research in Astronomy and Astrophysics, RAA 14 (2014) 1173-1192, 20 pages, 10 figure

Cryopreservation of Brassia rex Orchid Shoots Using PVS2 Technique

In vitro grown shoots of Brassia rex orchid hybrid was cryopreserved by means of plant vitrification solution 2 (PVS2) technique. For the preculture treatment, the shoots were excised into two standard sizes of 0.5-1.0 and 1.0-1.5 cm and were precultured on half-strength Murashige and Skoog (MS) semi solid medium supplemented with different concentrations of sucrose (control (0.06 M), 0.1, 0.25, 0.5 and 0.75 M) for 24 and 48 h. For the PVS2 dehydration treatment, the 0.1 M precultured (48 h and 1.0-1.5 cm) shoots were chosen for further experiment where the shoots were dehydrated in PVS2 solution at various durations (5, 10, 15, 20, 25 and 30 min) at 0 and 24°C for positive and negative storage in Liquid Nitrogen (LN). The viability of the cryopreserved cells were determined by 2, 3, 5-triphenyltetrazolium chloride (TTC) assay and chlorophyll extraction techniques. The best condition of PVS2 treatment was at 20 min of PVS2 treatment at 0°C prior to storage in liquid nitrogen. In chlorophyll determination based on chlorophyll assay, the highest concentration of total chlorophyll concentration (56.250 µg g-1) was obtained from shoots that were dehydrated for 25 min in PVS2 solution at 0°C without storage in liquid nitrogen

Tau aggregation and progressive neuronal degeneration in the absence of changes in spine density and morphology after targeted expression of Alzheimer's disease-relevant tau constructs in organotypic hippocampal slices

Alzheimer's disease (AD) is characterized by progressive loss of neurons in selected brain regions, extracellular accumulations of amyloid beta, and intracellular fibrils containing hyperphosphorylated tau. Tau mutations in familial tauopathies confirmed a central role of tau pathology; however, the role of tau alteration and the sequence of tau-dependent neurodegeneration in AD remain elusive. Using Sindbis virus-mediated expression of AD-relevant tau constructs in hippocampal slices, we show that disease-like tau modifications affect tau phosphorylation at selected sites, induce Alz50/MC1-reactive pathological tau conformation, cause accumulation of insoluble tau, and induce region-specific neurodegeneration. Live imaging demonstrates that tau-dependent degeneration is associated with the development of a "ballooned" phenotype, a distinct feature of cell death. Spine density and morphology is not altered as judged from algorithm-based evaluation of dendritic spines, suggesting that synaptic integrity is remarkably stable against tau-dependent degeneration. The data provide evidence that tau-induced cell death involves apoptotic as well as nonapoptotic mechanisms. Furthermore, they demonstrate that targeted expression of tau in hippocampal slices provides a novel model to analyze tau modification and spatiotemporal dynamics of tau-dependent neurodegeneration in an authentic CNS environment