Education of ecological culture of school children on the basis of ethnopedagogy

© Medwell Journals, 2016.Of the pedagogical system based on knowledge of the Tatar people on the nature, implemented on the lessons of natural sciences and geography of Tatarstan is an interconnected set of means, methods and processes that contribute to the formation of ecological culture of the students. The funds of the national pedagogies, traditions, rites and customs of the people of their environmental content establish stable norms and rules of behavior of children in nature, contribute to the education of ecological culture

Changes in the urban population in the republic of the volga federal district of Russia

© Medwell Journals, 2016.The study considers the the dynamics of the urban population in the republics of the Volga Federal District (VFD) Russia: Bashkortostan, Mari El, Mordovia, Tatarstan, Udmurtia, Chuvashia from 1990-2013. The main factors affecting to the changing in the urban population in these republics: decline in industrial production; internal and external migration; deterioration of the socio-economic conditions of the population, especially in small cities and towns associated with unemployment; aggravation of environmental problems in large cities and others. Of the 6 republics of the VFD the positive dynamics of the urban population is observed only in the Republic of Tatarstan to 197 thous. people from 1990-2012. And in the other republics of VFD there are negative indicators of the dynamics of the urban population

HtrA Protease from Bacillus subtilis Suppresses the Bacterial Fouling of the Rat Skin Injuries

© 2016, Springer Science+Business Media New York.The gene of serine protease HtrA from Bacillus subtilis was cloned and recombinant protein was overexpressed in E. coli and purified. The recombinant HtrA efficiently suppressed in vitro the biofilm formation by clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis. While the model rat skin injuries treated with HtrA healed slower than in the case of chymotrypsin, their recovery was significantly faster compared with pure buffer. On the other hand, the number of bacterial CFUs on the injuries treated with HtrA solution was reduced five times in 8 days, similarly to chymotrypsin-treated ones, while only twofold reduction was observed in controls. By the way, the resident microflora content of protease-treated and control wounds remained almost similar within 4 days, with Enterococcus faecalis and S. epidermidis being the main resident microflora after the treatment. To the eighth day, the amount of staphylococcal cells was drastically reduced on HtrA- and chymotrypsin-treated wound surfaces, confirming that both proteases provide wound cleaning from pathogenic microflora. Thus, HtrA from B. subtilis significantly reduces the microbial fouling of the wound surface being thereby of interest for wound care

Membrane protein dynamics: limited lipid control

Correlation of lipid disorder with membrane protein dynamics has been studied with infrared spectroscopy, by combining data characterizing lipid phase, protein structure and, via hydrogen-deuterium (H/D) exchange, protein dynamics. The key element was a new measuring scheme, by which the combined effects of time and temperature on the H/D exchange could be separated. Cyanobacterial and plant thylakoid membranes, mammalian mitochondria membranes, and for comparison, lysozyme were investigated. In dissolved lysozyme, as a function of temperature, H/D exchange involved only reversible movements (the secondary structure did not change considerably); heat-denaturing was a separate event at much higher temperature. Around the low-temperature functioning limit of the biomembranes, lipids affected protein dynamics since changes in fatty acyl chain disorders and H/D exchange exhibited certain correlation. H/D exchange remained low in all membranes over physiological temperatures. Around the high-temperature functioning limit of the membranes, the exchange rates became higher. When temperature was further increased, H/D exchange rates went over a maximum and afterwards decreased (due to full H/D exchange and/or protein denaturing). Maximal H/D exchange rate temperatures correlated neither with the disorder nor with the unsaturation of lipids. In membrane proteins, in contrast to lysozyme, the onsets of sizable H/D exchange rates were the onsets of irreversible denaturing as well. Seemingly, at temperatures where protein self-dynamics allows large-scale H/D exchange, lipid-protein coupling is so weak that proteins prefer aggregating to limit the exposure of their hydrophobic surface regions to water. In all membranes studied, dynamics seemed to be governed by lipids around the low-temperature limit, and by proteins around the high-temperature limit of membrane functionality

Heat stress causes spatially-distinct membrane re-modelling in K562 leukemia cells

Cellular membranes respond rapidly to various environmental perturbations. Previously we showed that modulations in membrane fluidity achieved by heat stress (HS) resulted in pronounced membrane organization alterations which could be intimately linked to the expression and cellular distribution of heat shock proteins. Here we examine heat-induced membrane changes using several visualisation methods. With Laurdan two-photon microscopy we demonstrate that, in contrast to the enhanced formation of ordered domains in surface membranes, the molecular disorder is significantly elevated within the internal membranes of cells preexposed to mild HS. These results were compared with those obtained by anisotropy, fluorescence lifetime and electron paramagnetic resonance measurements. All probes detected membrane changes upon HS. However, the structurally different probes revealed substantially distinct alterations in membrane heterogeneity. These data call attention to the careful interpretation of results obtained with only a single label. Subtle changes in membrane microstructure in the decision-making of thermal cell killing could have potential application in cancer therapy

Membrane fluidity matters: Hyperthermia from the aspects of lipids and membranes

Hyperthermia is a promising treatment modality for cancer in combination both with radio- and chemotherapy. In spite of its great therapeutic potential, the underlying molecular mechanisms still remain to be clarified. Due to lipid imbalances and 'membrane defects' most of the tumour cells possess elevated membrane fluidity. However, further increasing membrane fluidity to sensitise to chemo-or radiotherapy could have some other effects. In fact, hyperfluidisation of cell membrane induced by membrane fluidiser initiates a stress response as the heat shock protein response, which may modulate positively or negatively apoptotic cell death. Overviewing some recent findings based on a technology allowing direct imaging of lipid rafts in live cells and lipidomics, novel aspects of the intimate relationship between the 'membrane stress' of tumour cells and the cellular heat shock response will be highlighted. Our findings lend support to both the importance of membrane remodelling and the release of lipid signals initiating stress protein response, which can operate in tandem to control the extent of the ultimate cellular thermosensitivity. Overall, we suggest that the fluidity variable of membranes should be used as an independent factor for predicting the efficacy of combinational cancer therapies

Environmental education of the younger generation

© 2015, Asian Social Science. All rights reserved. As socially-historic experience shows, non-acquaintance with the culture of your own nationality, its past and present means breaking up of ties with generations, that cripples in formation of pupils’ ecological culture and creation of ecological space. Tatar peoples’ family life, the way they bring up their children differ from others and it is affected by traditions, customs, holidays and everyday life of these people. The child is growing up and developing in a native verbal environment, traditions of his nation. Traditions, customs, pedagogical and ecological experience of people are in every field of life – economic, political, moral, common, pedagogical and ecological culture. They are peculiar to every culturally geographical regions and tribes

Environmental education of the younger generation

© 2015, Asian Social Science. All rights reserved. As socially-historic experience shows, non-acquaintance with the culture of your own nationality, its past and present means breaking up of ties with generations, that cripples in formation of pupils’ ecological culture and creation of ecological space. Tatar peoples’ family life, the way they bring up their children differ from others and it is affected by traditions, customs, holidays and everyday life of these people. The child is growing up and developing in a native verbal environment, traditions of his nation. Traditions, customs, pedagogical and ecological experience of people are in every field of life – economic, political, moral, common, pedagogical and ecological culture. They are peculiar to every culturally geographical regions and tribes

Environmental education of the younger generation

© 2015, Asian Social Science. All rights reserved. As socially-historic experience shows, non-acquaintance with the culture of your own nationality, its past and present means breaking up of ties with generations, that cripples in formation of pupils’ ecological culture and creation of ecological space. Tatar peoples’ family life, the way they bring up their children differ from others and it is affected by traditions, customs, holidays and everyday life of these people. The child is growing up and developing in a native verbal environment, traditions of his nation. Traditions, customs, pedagogical and ecological experience of people are in every field of life – economic, political, moral, common, pedagogical and ecological culture. They are peculiar to every culturally geographical regions and tribes

Formation of creative pedagogical system of continuous environmental education

The goal of this article is to form a creative pedagogical system of continuous environmental education as exemplified by the creative union of scientists and attendants of preprofessional and additional education, refresher and transition courses for teachers. The currently being formed system of continuous environmental education aids contextual education at the stage of higher professional education and professional advancement of teachers