Detection and Analysis of Lysozyme Activity in some Tuberous Plants and Calotropis Procera’s Latex

Tuber extract of all the plant species used in the study exhibited lysozyme activity confirming the ubiquitous presence of lysozyme in plants. Among the different plants screened for protein content the tuber extract of Solanum tuberosum showed highest buffer soluble protein while tuber extract of Raphanus sativus showed the lowest protein content in sodium acetate buffer (50 Mm; pH 5.0). Tuber extract of Raphanus sativus showed highest lysozyme activity among all the plant species tested in this study and the activity was increased when the tuber was extracted with sodium phosphate buffer (50mM; pH 7.0). The lowest lysozyme was observed with tuber extract of Daucus carota in phosphate buffer (50mM; pH 7.0). The latex of the tropical species Calotropis procera is well known for being a rich source of the lysozyme. Lysozyme of Calotropis procera latex is not thermo labile. It did not lose much of its activity when the latex was incubated at different temperatures for 24 hours. A positive pointer for purification of this enzyme in future. Calotropis procera lysozyme can be was specifically isolated and purified from the whole latex with ammonium sulphate precipitation with 95% saturation. Calotropis procera lysozyme retained its activity even after precipitation with ammonium sulphate and dialysis and could hydrolyse the cell wall of Micrococcus lysodeikticus.Key words: Micrococcus lysodeikticus, Tuberous Plants, Latex, Ammonium sulphate precipitation, Polyacrylamide gel electrophoresis M. Sakthivel et al. Detection and Analysis of Lysozyme Activity in some Tuberous Plants and Calotropis Procera’s Latex. J Phytol 2/11 (2010) 65-72

Screening, Identifying of Penicillium K-P Strain and Its Cellulase Producing Conditions

Cellulase production by Penicillium species are of greater interest in microbial enzyme technology. Penicillium sp. was cultivated in liquid culture medium at different carbon sources, nitrogen sources in different pH and temperature conditions. The strain of K-P with high cellulase activity was screened. The cellulase activity was 198 U/mL in the presence of fructose on day fifth. Maximum activity was recorded 154 U/mL in with the presence of ammonium nitrate on the fourth day. And maximum cellulase activity was obtained when the pH was 3.0 (129 U/mL) on day fourth. But the highest cellulase activity recorded (274 U/mL) in the presence of fructose, ammonium nitrate, pH 3.0 on the fifth day. The results showed the profiles of cellulase were produced maximum level according to which enzyme is most active in that particular environment

Optimization of Culture Conditions for the Production of Extracellular Cellulase from Corynebacterium lipophiloflavum

Cellulolytic enzyme producing bacteria were isolated from decaying vegetables on nutrient agar plates. As many as fourteen different bacterial strains were isolated and they were screened for the production of cellulase enzyme on a medium containing carboxymethyl cellulose (0.5% w/v). A Congo red dye based qualitative assay was performed to identify cellulase producing bacteria isolated from the vegetables. Among the difference strains, after screening on CMC agar, one strain exhibited relatively higher cellulase activity and was identified as Cornybacterium lipophiloflavum by a bacteriologist. Culture conditions were optimized for the above bacterium in the production medium supplemented with different carbon and nitrogen sources, different pH and different concentrations of CMC. Glucose and yeast extract proved to be the suitable carbon and nitrogen sources while pH 7.0 was ideal for cellulase production by the bacterium. Carboxymethyl cellulose at 1% in the medium induced relatively higher cellulase activity. Electrophoretic analysis of the ammonium sulfate precipitated proteins showed three prominent bands and whose molecular masses were estimated as 60, 69 and 75 kDa.  Cellulase produced by Cornybacterium lipophiloflavum could efficiently remove dyes or inks from the pulp incubated either in the form of intact growing cell or with clarified supernatant. The enzyme also removed starch from the fabric incubated with culture supernatant suggesting the potential use of Cornybacterium lipophiloflavum cellulase in industrial applications

Optimization of Culture Conditions for the Production of Extracellular Cellulase from Enterococcus pseudoavium

Bacteria that inhabit decaying vegetables were isolated and screened for the production of the enzyme, amylase. One species identified as Enterococcus pseudoavium exhibited relatively higher amylase activity of the bacterial species tested. The bacterium produced the highest extracellular amylase at 72 h in a medium containing starch (1% w/v), galactose (0.5% w/v), and peptone (0.5% w/v), at pH 7.0. The extracellular enzyme was partially purified and its starch hydrolyzing potential was evaluated. The organism when grown with paper pulp deinked the pulp completely just after four days of growth. The bacterial cells immobilized in sodium alginate beads when cultured with paper pulp could decolorize it within 4 days. The extracellular amylase produced by Enterococcus pseudoavium effectively deinked and decolorized paper pulp within 4 days after incubation. The enzyme efficiently removed the starch present in fabric and thus it could be very well used as an ingredient in commercial detergent

Padina boergessenii ameliorates carbon tetrachloride induced nephrotoxicity in Wistar rats

AbstractThis research investigates the ameliorative effect of brown alga Padina boergessenii against CCl4-induced nephrotoxicity in rats. Twenty-four Wistar albino rats were divided into four groups as follows: control group (saline), CCl4 group, CCl4+P. boergessenii extract group and P. boergessenii extract alone group. At the end of the experimental period, the animals are sacrificed and tissue samples (kidney) were collected. CCl4 at a dose of 2mL/kg, administered subcutaneously, produced significantly decreased activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT). Histopathological examinations showed massive centrilobular necrosis and fat accumulation in CCl4-induced animals. In the protective test, especially in animals pretreated with P. boergessenii (150mg/kg) extract, there was a significant increase in antioxidant enzyme levels although there was no sign of abnormality in the kidney of rats pretreated with extract as evidenced by histopathological sections against CCl4-induced decrease of lipid peroxidation (TBARS). The ameliorative effect may be due to the presence of various bioactive compounds in alga. These results suggest that an extract of P. boergessenii is able to significantly alleviate the nephrotoxicity induced by CCl4 in Wistar rats

3-(7,8,13,14-Tetra­hydrodi­benzo­[a,i]phen­an­thridin-5-yl)benzene-1,2-diol

In the title compound, C27H21NO2, the half-chair conformation of the alicyclic rings gives rise to a slightly folded structure of the central tricyclic tetra­hydrophenanthridine unit. Tandem intra­molecular O—H⋯N and O—H⋯O hydrogen bonds give rise to adjacent S(6) and S(5) rings, respectively, which dictate the conformation of the 5-aryl substituent. In the crystal structure, an inter­molecular C—H⋯O contact generates chains parallel to [101]. Short O—H⋯π and C—H⋯π contacts are also observed

Time series modeling for forecasting the adoption behaviour of shrimp farmers

Commercial shrimp culture has emerged as a prominent sector of the coastal economy of India by virtue of the foreign exchange returns, rural employment it generates, and the economic viability of the enterprise and high market demand for the produce. The present study was conducted in Nellore and Nagapattinam districts a/India 10 forecast the adoption a/scientific technologies for a period of 17 years from 2004-2020, by the shrimp farmers using time series statistical modeling. The background data collected from 1997 to 2003 on the adoption behaviour of the farmers formed the database for predicting the adoption behaviour of the technologies. The study revealed that of the two statistical methods employed, namely Holt linear model and Holt exponential smoothing model, the goodness of fit generated by the Holt linear model with an R2 value 0/0.97 exhibited a higher degree of model adequacy over the Holt exponential smoothing model which revealed that in the years 2019 and 2020, the overall extent of adoption exceeded 100 percent which implies the development of new technologies by the research system and its subsequent adoption by the farmers. Besides factors such as changes in demography, demands in the export and local markets would motivate the shrimp fanners in future to adopt more of the improved technologies for getting higher yields

(3E,5E)-1-Benzyl-3,5-bis­(2-fluoro­benzyl­idene)piperidin-4-one

The inversion-related mol­ecules of the title compound, C26H21F2NO, associate into closed dimeric subunits via co-operative C—H⋯π inter­actions. Two non-classical C—H⋯O and one C—H⋯N intra­molecular hydrogen bonds are also found in the crystal structure. The piperidin-4-one ring adopts a sofa conforamtion with the 1-benzyl group in the equatorial position, and the equiplanar fluoro­phenyl substituents in the 3- and 5-positions stretched out on either side. The 1-benzyl group is disposed towards the substituent in the 6th position of the piperidin-4-one ring. The 3,5-diene units possess E configurations

Changes in biochemical constituents and antioxidant enzyme activity in groundnut (Arachis hypogaea L.) by the addition of coated multi-nutrient fertilization in calcareous soil

Sulphur and micronutrients play a vital in the growth and development of plants due to their catalytic effect on many metabolic processes. A field experiment was conducted to explore the changes in various biochemical constituents and antioxidants enzyme activities in response to coated multi-nutrient fertilization. The experiment consisted of five organic acids (citric acid, humic acid, fulvic acid, salicylic acid) and amino acid (glycine) coated multi-nutrient fertilizer sources applied at five different levels (0, 5, 10, 12.5 and 15 kg ha-1). Groundnut leaf samples were collected and analyzed for biochemical constituents such as proline, soluble protein and antioxidant enzymes viz., superoxide dismutase, catalase, peroxidase and carbonic anhydrase activities at harvest stages. The results revealed that, application of fulvic acid coated multi-nutrient fertilizer at 15 kg ha-1 registered lesser proline (5.93 µmoles g-1) and higher soluble protein (22.2 mg g-1) content, superoxide dismutase (8.93 EU mg-1), catalase (18.2 µg H2O2 min-1 g˗1), peroxidase (6.11 µg min-1 mg˗1) and carbonic anhydrase (14.8 EU mg-1) activities at harvest stage followed by 12.5 kg humic acid coated multi-nutrient fertilizer. The lesser response was noted with NPK control in influencing the biochemical constituents and antioxidant enzymes. It was concluded that fulvic coated multi-nutrient fertilizer at 15 kg ha-1 was the better source for improving the biochemical constituents and antioxidant enzymes of groundnut in calcareous soils

(3E,5E)-3,5-Bis(4-allyl­oxybenzyl­idene)-1-benzyl­piperidin-4-one

In the title compound C32H31NO3, the all­yloxy groups on either side of the piperidin-4-one ring are conformationally disordered. The contribution of major and minor components of the allyloxy group at the 3rd position of the ring are 0.576 (4) and 0.424 (4), respectively, and those at the 5th position are 0.885 (3) and 0.115 (3), respectively. The six-membered piperidin-4-one ring adopts a sofa conformation with the benzyl group occupying an equatorial position and the olefinic double bonds possessing an E configuration. Flanking phenyl substituents are stretched out on either side of the six-membered ring. π–π inter­actions with a centroid–centroid distance of 3.885 (1) Å give rise to mol­ecular dimers and short C—H⋯π contacts lead to chains along the c axis