The basic laparoscopic skills long-term survival: new prediction scale

Introduction: The most important principle of pedagogy, including medical, is to correctly assess the knowledge and skills acquisition since they must be restored during the study and retained for use in further professional practice. Knowledge and skills in time survival are of particular importance in medicine since its determination allows to reveal the necessary time for repeated training and evaluate in general the medical education system efficiency. Objective: To develop a scale for predicting basic laparoscopy skills long-term survival in the medical education system. Materials and methods: The training results and assessment of 48 Odessa National Medical University medical students have been studied with the use of basic laparoscopy skills module of 3-D laparoscopy simulator trained as part of this module at the 5th year, forming a comparative group - CG (for obtaining initial mathematical prediction indicators), repeated it at the 6th year - forming the main group - MG (this group for the knowledge survival calculations and for reaching the main goal). They have passed all the module tasks, at least 10 trainings per module with the number of repetitions from 1 to 4. Time was recorded for the practical skills, security parameters, visual-motor coordination, the selection and release of devices, pedaling, diathermy, aspiration, irrigation, with a video camera viewing angle of 30° and 0°. The definition of the initial (1st training), intermediate (5th training) and the final (10th training) level of the CG and the MG students practical skills during two years of training, forming 6 groups accordingly, using the self-assessment coefficient (SAC) of the trainee, developed by us on the questionnaires and the practical skills coefficient (PSC) based on the evaluation sheets was determined, summarized and generalized for each group. The Lykert 6-point scale (0-5) of competence levels total assessments were used

3D, haptics and virtual reality technologies implementation results in quality assessment and assurance of obstetrician-gynecologists’ hysteroscopy training

Modern practical gynecology requires mandatory diagnostic and therapeutic hysteroscopic (HS) procedures. Their high-quality and safe performance is due to repeated trainings for mastering motor skills. This should ultimately lead to a tactile sensation and understanding of depth, fulcrum and force of impact. Simulation training is especially important, which gives effective theoretical and practical training of novice gynecologists for basic HS manipulations, the ability to conduct and evaluate their teamwork. The virtual simulator is widely used for objective assessment of clinical skills and abilities associated with the competence

Innovative methods efficiency in obstetricians-gynecologists’ postgraduate education

Introduction: Important for the Ukraine was the creation of the new reformative governmental undergraduate and postgraduate medical education program. In Odessa State Medical University today it’s the main innovative guideline for the physician modern practical training especially in obstetrics and gynecology. Objective: To determine the efficiency of the innovative methods in the obstetricians-gynecologists postgraduate education. Materials and methods: The learning outcomes and assessment of 320 postgraduates of OB/GYN have been studied with the help of imitational virtual platforms for the obstetricians-gynecologists, birth simulators, virtual operation room and virtual labor room. Physicians’ average age was 39,4 ± 0,7 years; their average work experience - 12,6 ± 0,9 years. They underwent seminar (10%) and practical (90%) classes for the normal and pathological labor, obstetrical operations, urgent cases and emergencies in obstetrics with several different assessment protocols, including initial and final testing, anonymous self-assessment for their practical skills, structured check-lists with intermediate and final steps for each practical skill (from 12 to 18 points), team work with changing roles using video-monitoring and debriefing by case-study check-lists (29 positions; from 2 to 5 points for each). Results: According to the results of the final tests it was found that low initial assessment of complicated childbirth and obstetric surgery themes significantly increased one and a half times (р <0,001). After completion of the course the theoretical training was improved by one and a half times (р <0,05). Indicators of the output of the theoretical test of postgraduates with work experience of at least five years, compared with the postgraduates, the experience of more than fifteen years, were 1.3 times lower (р < 0,001). On the course completion the theoretical performance testing has increased one and a half times, and skills have increased twice. Initial assessment of the vacuum extraction newborn operation significantly increased almost twice (р <0,001). Teamwork evaluation at the end of the course has increased more than twice (р <0,001)

OVERVIEW OF POPULAR APPROACHES IN CREATING CLIENT-SERVER APPLICATIONS BASED ON SCIENTOMETRICS ONAFTS’ PLATFORM

Most of the currently developed systems are based on the client-server architecture. This architecture is usedeverywhere, from mobile-native development to Web applications.However implementing an application based on this architectural solution requires quite a lot of effort from the softwaredeveloper, and therefore, in order to simplify and speed up the development, certain standard solutions and approachesappeared. This article will discuss the most popular technologies used in the development of Web applications in the context ofenterprise development.Also in this article will be mentioned the project, built on the architecture of "client-server" - ScienceToMetrics.The main theme of this project is the study of science-metric indicators for the structural divisions of the faculty of theOdessa National Academy of Food Technologies. In fact, it is a portal for viewing and editing information on employees, inthe future this portal may be extended to subprojects.In this project, the main idea of this architecture was embodied: decomposition of the application into atomic parts inorder to distribute it to several hardware units of capacity to improve performance. The client is an independent application,which at the same time receives information from an external API-interface through REST-requests. In turn, the backendprovides this API with certain security restrictions on the content provided. The backend for this architecture provides a layerfor the content of the data users, whether it's a database (NoSQL, SQL) or an integration API with external aggregationsystems. To ensure the necessary level of security, JWT (Javascript Web Token) authorization is used, which allows you not tocreate an explicit session between the client and the backend, but allows you to communicate through a token that stores allthe necessary meta-information for this user.</p

Methods for Efficient Elimination of Mitochondrial DNA from Cultured Cells.

Here, we document that persistent mitochondria DNA (mtDNA) damage due to mitochondrial overexpression of the Y147A mutant uracil-N-glycosylase as well as mitochondrial overexpression of bacterial Exonuclease III or Herpes Simplex Virus protein UL12.5M185 can induce a complete loss of mtDNA (ρ0 phenotype) without compromising the viability of cells cultured in media supplemented with uridine and pyruvate. Furthermore, we use these observations to develop rapid, sequence-independent methods for the elimination of mtDNA, and demonstrate utility of these methods for generating ρ0 cells of human, mouse and rat origin. We also demonstrate that ρ0 cells generated by each of these three methods can serve as recipients of mtDNA in fusions with enucleated cells

COVID-19: Effectiveness Evaluation New Concept of Combined Learning for Ob/Gyn Residents Under Quarantine Restrictions

Materials and methods. The research involved 139 residents who studied at the Department of Obstetrics and Gynecology ONMedU in 2020-2021 under the new concept of combined learning due to quarantine restrictions (Group I), and 132 OB / GYN residents who studied in 2018 - 2019 for traditional full-time educational program (group II). The learning outcomes and assessment of 139 residents of OB / GYN (І group) have been analyzed with the help of imitational virtual platforms for obstetriciansgynecologists, birth simulators, virtual operation room and virtual labor room

Changes in the microbiome in women with polycystic ovary syndrome

Мікробіом кишечника (МК) активно вивчається в розрізі його впливу на розвиток онкологічної патології, імунних захворювань і порушень обміну речовин. Жінки із синдромом полікістозних яєчників (СПКЯ) мають дисбаланс МК, що опосередковано впливає на слизовий бар’єр кишечника. Порівняно з пацієнтками без ожиріння та здоровою контрольною популяцією, в осіб із СПКЯ на тлі ожиріння збільшується кількість ентеробактерій, зменшується кількість лактобактерій та біфідобактерій, а зміни МК пов’язані з рівнем запалення та резистентністю до інсуліну. Трансплантація фекальної мікробіоти є новим методом лікування запальних захворювань кишечника. Регулювання МК для покращення метаболізму СПКЯ може бути одним із потенційних варіантів майбутнього лікування СПКЯ, але конкретний механізм його дії ще належить дослідити поглиблено. Дедалі більше доказів свідчать, що пробіотики, пребіотики та синбіотики є ефективними варіантами лікування пацієнток із СПКЯ. Дослідження показують, що пробіотики можуть відновити різноманітність кишкової мікробіоти мишей із СПКЯ, зменшити порушення флори та покращити репродуктивну функцію мишей. Змінений мікробіом нижніх статевих шляхів при СПКЯ може спричинити зміни, які пов’язані з рівнем гормонів протягом менструального циклу. Дослідження свідчать, що вагінальний мікробіом жінок із СПКЯ відрізняється від здорових жінок тим, що в ньому домінують Mycoplasma, Prevotella, Gardnerella, Actinomyces, Enterococcus та Atopobium. Рівень жіночих статевих гормонів пов’язаний зі складом мікробіому ротової порожнини, яка асоціюється з такою патологією порожнини рота, як пародонтоз. Дослідження патогенних мікроорганізмів у періодонті та їхньої поширеності у жінок із СПКЯ показало, що здорові жінки мають вищі рівні Peptostreptococcus а також виявлено більший відсоток жінок, інфікованих Treponema denticola. Новітні погляди на лікування жінок із СПКЯ дедалі більше вказують на важливість урахування змін мікробіому та відкривають нові терапевтичні можливості. Дослідження в цій сфері тривають, а для уточнення мікробного складу у жінок із СПКЯ необхідні додаткові дослідження.The gut microbiome (GM) is actively studied in terms of its impact on the development of oncological pathology, immune diseases, and metabolic disorders. Women with polycystic ovary syndrome (PCOS) have an imbalance of GM, which indirectly affects the intestinal mucosal barrier. Compared with non-obese patients and a healthy population, obese individuals with PCOS have increased numbers of Enterobacteriaceae, decreased numbers of Lactobacillus and Bifidobacterium, and changes in GM are associated with inflammation and insulin resistance. Fecal microbiota transplantation is a new method of treating inflammatory bowel diseases. GM regulation to improve the metabolism of PCOS may be one of the potential options for the future treatment of PCOS, but the specific mechanism of its action remains to be investigated. There is increasing evidence that probiotics, prebiotics, and synbiotics are effective treatment options for PCOS patients. Studies show that probiotics can restore the diversity of the GM of mice with PCOS, reduce the disruption of the flora and improve the reproductive function of the mice. The altered microbiome of the lower genital tract in PCOS can cause changes that are related to hormone levels during the menstrual cycle. Studies show that the vaginal microbiome of women with PCOS differs from healthy women due to the domination of Mycoplasma, Prevotella, Gardnerella, Actinomyces, Enterococcus, and Atopobium. The level of female sex hormones is associated with the composition of the oral cavity microbiome, which is associated with such oral pathology as periodontal disease. A study of periodontal pathogens and their prevalence in women with PCOS found that healthy women had higher levels of Peptostreptococcus and a higher percentage of women infected with Treponema denticola. New views on the management of women with PCOS indicate the importance of considering microbiome changes and open up new therapeutic opportunities. Research in this area is still ongoing, and additional studies are needed to clarify the microbial composition in women with PCOS

Growth characteristics of the ρ⁰ cells.

<p>A, representative field views of the ρ⁰ cells generated with either ExoIII or mUNG1 after seeding (0d) and after growth for 5 days in different media (5d). Seed, number of cells per well at 0d. +/- UP, DMEM medium either supplemented or not with uridine and pyruvate, respectively. B and C, growth curves for the parental (WT) and ρ⁰ cells in different media. D, doubling times for the parental and ρ⁰ cells in different media. Results are mean ± SEM of three biological replicas. Error bars are smaller than symbols.</p