842 research outputs found
Comparative gene expression analysis of susceptible and resistant near-isogenic lines in common wheat infected by Puccinia triticina
Gene expression after leaf rust infection was compared in near-isogenic wheat lines differing in the Lr10 leaf rust resistance gene. RNA from susceptible and resistant plants was used for cDNA library construction. In total, 55 008 ESTs were sequenced from the two libraries, then combined and assembled into 14 268 unigenes for further analysis. Of these ESTs, 89% encoded proteins similar to (E value of < or =10(-5)) characterized or annotated proteins from the NCBI non-redundant database representing diverse molecular functions, cellular localization and biological processes based on gene ontology classification. Further, the unigenes were classified into susceptible and resistant classes based on the EST members assembled from the respective libraries. Several genes from the resistant sample (14-3-3 protein, wali5 protein, actin-depolymerization factor and ADP-ribosylation factor) and the susceptible sample (brown plant hopper resistance protein, caffeic acid O-methyltransferase, pathogenesis-related protein and senescence-associated protein) were selected and their differential expression in the resistant and susceptible samples collected at different time points after leaf rust infection was confirmed by RT-PCR analysis. The molecular pathogenicity of leaf rust in wheat was studied and the EST data generated made a foundation for future studies
Nonet Symmetry and Two-Body Decays of Charmed Mesons
The decay of charmed mesons into pseudoscalar (P) and vector (V) mesons is
studied in the context of nonet symmetry. We have found that it is badly broken
in the PP channels and in the P sector of the PV channels as expected from the
non-ideal mixing of the \eta and the \eta'. In the VV channels, it is also
found that nonet symmetry does not describe the data well. We have found that
this discrepancy cannot be attributed entirely to SU(3) breaking at the usual
level of 20--30%. At least one, or both, of nonet and SU(3) symmetry must be
very badly broken. The possibility of resolving the problem in the future is
also discussed.Comment: 9 pages, UTAPHY-HEP-
Magnetic Exciton Mediated Superconductivity in the Hidden-Order Phase of URu2Si2
We propose the magnetic exciton mediated superconductivity occurring in the
enigmatic hidden-order phase of URu2Si2. The characteristic of the massive
collective excitation observed only in the hidden-order phase is well
reproduced by the antiferro hexadecapole ordering model as the trace of the
dispersive crystalline-electric-field excitation. The disappearance of the
superconductivity in the high-pressure antiferro magnetic phase can naturally
be understood by the sudden suppression of the magnetic-exciton intensity. The
analysis of the momentum dependence of the magnetic-exciton mode leads to the
exotic chiral d-wave singlet pairing in the Eg symmetry. The Ising-like
magnetic-field response of the mode yields the strong anisotropy observed in
the upper critical field even for the rather isotropic 3-dimensional Fermi
surfaces of this compound.Comment: 5 pages, 4 figure
Correlation gap in the heavy-fermion antiferromagnet UPd_2Al_3
The optical properties of the heavy-fermion compound UPdAl have been
measured in the frequency range from 0.04 meV to 5 meV (0.3 to 40 cm) at
temperatures K. Below the coherence temperature K, the hybridization gap opens around 10 meV. As the temperature decreases
further ( K), a well pronounced pseudogap of approximately 0.2 meV
develops in the optical response; we relate this to the antiferromagnetic
ordering which occurs below K. The frequency dependent mass and
scattering rate give evidence that the enhancement of the effective mass mainly
occurs below the energy which is associated to the magnetic correlations
between the itinerant and localized 5f electrons. In addition to this
correlation gap, we observe a narrow zero-frequency conductivity peak which at
2 K is less than 0.1 meV wide, and which contains only a fraction of the
delocalized carriers. The analysis of the spectral weight infers a loss of
kinetic energy associated with the superconducting transition.Comment: RevTex, 15 pages, 7 figure
Precise study of the resonance at Q0=(1,0,0) in URu2Si2
New inelastic neutron scattering experiments have been performed on URu2Si2
with special focus on the response at Q0=(1,0,0), which is a clear signature of
the hidden order (HO) phase of the compound. With polarized inelastic neutron
experiments, it is clearly shown that below the HO temperature (T0 = 17.8 K) a
collective excitation (the magnetic resonance at E0 \approx 1.7 meV) as well as
a magnetic continuum co-exist. Careful measurements of the temperature
dependence of the resonance lead to the observation that its position shifts
abruptly in temperature with an activation law governed by the partial gap
opening and that its integrated intensity has a BCS-type temperature
dependence. Discussion with respect to recent theoretical development is made
Evolution of Heterogeneous Antiferromagnetic State in URu2Si2: Study of Hydrostatic-Pressure, Uniaxial-Stress and Rh-Dope Effects
We have investigated the nature of the competition between hidden order and
antiferromagnetic (AF) order in URu_2Si_2 by performing the neutron scattering
experiments under hydrostatic-pressure P, uniaxial-stress sigma, and
Rh-substitution conditions. Hidden order observed at ambient pressure in pure
URu_2Si_2 is found to be replaced by the AF order by applying P, sigma along
the tetragonal basal plane, and by doping Rh. We discuss these experimental
results on the basis of the crystalline strain calculations, and suggest that
this phase transition is generated by the 0.1% increase of the tetragonal c/a
ratio. We have also found that the magnetic excitation observed in the hidden
order phase vanishes in the AF phase. We show that this variation can be
qualitatively explained by assuming the hidden order parameter to be
quadrupole.Comment: 4 pages, 4 figures, proceedings of workshop on Novel Pressure-Induced
Phenomena In Condensed Matter Systems, 2006 Fukuok
Quasiparticle Inelastic Lifetime from Paramagnons in Disordered Superconductors
The paramagnon contribution to the quasiparticle inelastic scattering rate in
disordered superconductors is presented. Using Anderson's exact eigenstate
formalism, it is shown that the scattering rate is Stoner enhanced and is
further enhanced by the disorder relative to the clean case in a manner similar
to the disorder enhancement of the long-range Coulomb contribution. The results
are discussed in connection with the possibility of conventional or
unconventional superconductivity in the borocarbides. The results are compared
to recent tunneling experiments on LuNiBC.Comment: 5 pages, no figure
Comparative Genetic Mapping and Discovery of Linkage Disequilibrium Across Linkage Groups in White Clover (Trifolium repens L.)
White clover (Trifolium repens L.) is an allotetraploid species (2n = 4X = 32) that is widely distributed in temperate regions and cultivated as a forage legume. In this study, we developed expressed sequence tag (EST)–derived simple sequence repeat (SSR) markers, constructed linkage maps, and performed comparative mapping with other legume species. A total of 7982 ESTs that could be assembled into 5400 contigs and 2582 singletons were generated. Using the EST sequences that were obtained, 1973 primer pairs to amplify EST-derived SSR markers were designed and used for linkage analysis of 188 F1 progenies, which were generated by a cross between two Japanese plants, ‘273-7’ and ‘T17-349,’ with previously published SSR markers. An integrated linkage map was constructed by combining parental-specific maps, which consisted of 1743 SSR loci on 16 homeologous linkage groups with a total length of 2511 cM. The primer sequences of the developed EST-SSR markers and their map positions are available on http://clovergarden.jp/. Linkage disequilibrium (LD) was observed on 9 of 16 linkage groups of a parental-specific map. The genome structures were compared among white clover, red clover (T. pratense L.), Medicago truncatula, and Lotus japonicus. Macrosynteny was observed across the four legume species. Surprisingly, the comparative genome structure between white clover and M. truncatula had a higher degree of conservation than that of the two clover species
HCV IRES manipulates the ribosome to promote the switch from translation initiation to elongation.
The internal ribosome entry site (IRES) of the hepatitis C virus (HCV) drives noncanonical initiation of protein synthesis necessary for viral replication. Functional studies of the HCV IRES have focused on 80S ribosome formation but have not explored its role after the 80S ribosome is poised at the start codon. Here, we report that mutations of an IRES domain that docks in the 40S subunit's decoding groove cause only a local perturbation in IRES structure and result in conformational changes in the IRES-rabbit 40S subunit complex. Functionally, the mutations decrease IRES activity by inhibiting the first ribosomal translocation event, and modeling results suggest that this effect occurs through an interaction with a single ribosomal protein. The ability of the HCV IRES to manipulate the ribosome provides insight into how the ribosome's structure and function can be altered by bound RNAs, including those derived from cellular invaders
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