Nickel nanoparticles induces cytotoxicity, cell morphology and oxidative stress in bluegill sunfish (BF-2) cells

The rationale of the current study was to assess the suitability of BF-2 cell line as a model to assess nanotoxicity in the caudal fin cells of bluegill sunfish in vitro. The current study investigates the potential toxicity, morphological changes and oxidative stress of nickel nanoparticles (Ni NPs) in bluegill sunfish cells (BF-2) using mitochondrial, neutral red uptake and lactate dehydrogenase assays. Results indicated a concentration dependent cytotoxic effect after 24 h in mitochondrial, lysosomal and lactate dehydrogenase activities. BF-2 cells morphology was altered when exposed to 30 μg ml−1 concentrations of Ni NPs for 24 h. Dose dependent increase of oxidative stress was evidenced in BF-2 cells when exposed to Ni NPs, showed significant escalation in peroxidation of lipids (LPO), protein carbonyl (PC), glutathione sulfo-transferase (GST) and glutathione peroxidase (GPX) as compared to their experimental controls. However, the catalase (CAT) and total glutathione content (TGSH) was found to decrease dose dependently in BF-2 cells exposed to Ni NPs. The current study demonstrated that BF-2 cells may serve as a sensitive indicator for aquatic contaminant evaluations in toxicological research.publishe

Mapping the protein phosphorylation sites in human mitochondrial complex I (NADH: ubiquinone oxidoreductase): A bioinformatics study with implications for brain aging and neurodegeneration

This file contains (1) the multiple sequence alignment of all the subunits of mitochondrial complex I cross different species (2) list of all the potentially phosphorylated resides along with the prediction score and (3) the list of all conserved, semi-conserved, conserved-substitute and non-conserved residues- ser, thr and tyr in different subunits of mitochondrial complex I

Mapping the protein phosphorylation sites in human mitochondrial complex I (NADH: ubiquinone oxidoreductase): A bioinformatics study with implications for brain aging and neurodegeneration

This file contains (1) the multiple sequence alignment of all the subunits of mitochondrial complex I cross different species (2) list of all the potentially phosphorylated resides along with the prediction score and (3) the list of all conserved, semi-conserved, conserved-substitute and non-conserved residues- ser, thr and tyr in different subunits of mitochondrial complex I

A clinical study on fournier’s gangrene

Aims and objectives: Fournier’s gangrene is a life threatening condition Is a synergistic infectious disease with necrotizing fasciitis of perineum, scrotum and penis. This study was conducted to evaluate the risk factors, bacteriology of infection, extent of involvement, management and prognosis. Methods: Prospective cohort study conducted in Department of General surgery, Govt General Hospital, Ongole, during the period of October 2017 to September 2020 were evaluated . Results :Patients diagnosed as fournier ‘s gangrene were studied in Govt General Hospital Ongole. Diabetes and alcoholism are the common risk factors . Diagnosis is mainly by clinical examination. Most of the cases presented between 48 hrs to 1 week. Radical debridement, antibiotic therapy follow up debridements are needed. FGSI score is helpful in evaluating prognosis. Mortality is high

Growth and Yield of Rice as Influenced by Rice Husk Biochar and Nitrogen Levels during Kharif Season

Aim: Impact of rice husk biochar and nitrogen levels on growth and yield of kharif rice. Study Design: The experiment was laid out in a spilt plot design with different biochar doses in main plots and different nitrogen levels in subplots and was replicated thrice. Place and Duration of Study: The field experiment was conducted during the kharif season of 2020 and 2021 at the Agricultural College Farm, Bapatla, ANGRAU, Lam, Guntur, Andhra Pradesh. Methodology: The experiment was performed with twenty treatments in a split-plot design. The main plot comprised four biochar treatments and a subplot with five different nitrogen levels. Rice variety "BPT-5204" was taken as the test variety. The crop and soil observations during the experimentation were recorded at regular intervals. The test examined the significance of the treatment impact. Results: Results of the experiment revealed that among the biochar treatments, the application of biochar @ 7.5 t ha-1 treatment showed the highest plant growth parameters at harvest like plant height (104.8 and 107.0 cm), number of tillers m-2 (361.6 and 369.6), leaf area index (3.69 and 3.71) and yield parameters, i.e., the number of panicles m-2 (302.5 and 304.9), grain (5706 and 5860 kg ha-1) and straw yields (7347 and 7570 kg ha-1) and was found statistically at par with the application of biochar @ 5.0 t ha-1 treatment and significantly superior over rest of the treatments during kharif of 2020 and 2021. Plant height (106.2 and 108.1 cm), number of tillers m-2 (368.3 and 374.2), leaf area index (3.72 and 3.84), the number of panicles m-2 (284.9 and 289.6), grain yield (5865 and 5997 kg ha-1), and straw yield (7631 and 7848 kg ha-1) were significantly the highest with the application of 160 kg N ha-1, which was found statistically at par with 120 kg N ha-1 treatment and significantly superior over other treatments