Supporting local authorities to plan energy efficiency in public buildings: from local needs to regional planning

The support offered to local authorities in this work consisted of technical and economic training on the integration of energy efficiency measures as well as the development of tools (guidelines, a decision support tool, databases and a visualization platform) that allow local authorities to prepare their building renovation plans. These tools are found very useful for local authorities, particularly in the case of Teruel province, characterised by many small municipalities whose local governments do not have the technical staff to undertake this type of planning. As a result of this work, an energy action plan was elaborated for the implementation of 96 energy efficiency measures in public buildings of Teruel province. The execution of this plan would allow energy savings of 1.3 MWh/year and 245 tonsCO2e/year of CO2 emission reduction, involving an investment of EUR 1.2 M. The close collaboration with public authorities made it possible to assess the strengths and weaknesses of using the developed tools. One of the barriers found is the availability of accurate building data (e.g., regarding envelope features) necessary for the decision support tool

The challenges of solar hybrid PVT systems in the food processing industry

This paper assesses the challenges of alternative solar systems based on hybrid PVT collectors coupled with an absorption chiller (AbCH, single-stage NH3-H2O) in the food-processing industry, from the technical, economic and environmental points of view. This type of industry is usually characterised by a constant cooling demand throughout the year, hot water demand for production processes and electricity consumption for factory equipment and lighting. To the authors’ knowledge, this work constitutes one of the first studies to address the integration of PVT-water collectors with a single-stage NH3-H2O AbCH for industrial applications. Two alternative PVT-water collectors are analysed, covered and uncovered. A biomass boiler is proposed as an auxiliary heater. To compare the proposed solar solutions, a vegetable and fruit processing and canning factory is considered as a representative case study. Hourly transient simulations considering the real factory demands and real weather data are performed over a year. Two main challenges are found for the solar systems based on the covered PVT collectors, an AbCH and a biomass boiler: the overlapping of the cooling and hot water demands of the food-processing industry, and the high hot water temperatures required. If, alternatively, the current electrical chillers are retained, the system based on uncovered PVT collectors has a reasonable-to-attractive payback time (14.3 years). When the potential environmental benefit is quantified (through carbon pricing), all the proposed solar systems become economically attractive, i.e., with positive total cost savings at the end of the system lifetime. Still, the high cost of PVT collectors, along with the considerably lower price of fuels compared to electricity, hinder the potential of systems that displace fossil fuels

Experimental validation of a solar system based on hybrid photovoltaic-thermal collectors and a reversible heat pump for the energy provision in non-residential buildings

This work aims to validate a transient model of a solar hybrid pilot plant based on photovoltaic-thermal (PV-T) collectors integrated via thermal storage tanks with an air-to-water reversible heat pump (rev-HP). The pilot plant is in operation and provides space heating, cooling, domestic hot water (DHW) and electricity to an industrial building located in Zaragoza (Spain). The plant consists of eight uncovered PV-T collectors (2.6 kWe, 13.6 m2), two water tanks and a rev-HP with a nominal thermal power of 16 kW for heating and 10.5 kW for cooling. The validation results show that the transient model fits the experimental performance of the PV-T collectors, with an average error of -16% and 3%, for the thermal and electrical generation respectively. The accuracy of the estimated rev-HP performance depends on the operation mode. The estimated COP in cooling mode has an average error of 14%, while in heating mode has an average error of -10%. The results show that the integration of the thermal and electrical generation of the PV-T collectors with a high-performance rev-HP allows the solar PV-T system to be self-sufficient to satisfy the building energy demand

Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF

Increased expression of VEGF in several types of tumours has been shown to correlate with poor prognosis. We used a replication-deficient adenoviral vector containing antisense VEGF cDNA (Ad5CMV-αVEGF) to down-regulate VEGF expression and increase the efficiency of delivery of the antisense sequence in the human breast cancer cell line MDA231-MB. Transfection of these cells with Ad5CMV-αVEGF in vitro reduced secreted levels of VEGF protein without affecting cell growth. Moreover, injection of the Ad5CMV-αVEGF vector into intramammary xenografts of these cells established in nude mice inhibited tumour growth and reduced the amount of VEGF protein and the density of microvessels in those tumours relative to tumours treated with the control vector Ad5(dl312). Our results showed that antisense VEGF 165 cDNA was efficiently delivered in vivo via an adenoviral vector and that this treatment significantly inhibited the growth of established experimental breast tumours. The Ad5CMV-αVEGF vector may be useful in targeting the tumour vasculature in the treatment of breast cancer. © 2001 Cancer Research Campaign http://www.bjcancer.co

In silico-designed lignin peroxidase from Phanerochaete chrysosporium shows enhanced acid stability for depolymerization of lignin

Background: The lignin peroxidase isozyme H8 from the white-rot fungus Phanerochaete chrysosporium (LiPH8) demonstrates a high redox potential and can efficiently catalyze the oxidation of veratryl alcohol, as well as the degradation of recalcitrant lignin. However, native LiPH8 is unstable under acidic pH conditions. This characteristic is a barrier to lignin depolymerization, as repolymerization of phenolic products occurs simultaneously at neutral pH. Because repolymerization of phenolics is repressed at acidic pH, a highly acid-stable LiPH8 could accelerate the selective depolymerization of recalcitrant lignin. Results: The engineered LiPH8 was in silico designed through the structural superimposition of surface-active site-harboring LiPH8 from Phanerochaete chrysosporium and acid-stable manganese peroxidase isozyme 6 (MnP6) from Ceriporiopsis subvermispora. Effective salt bridges were probed by molecular dynamics simulation and changes to Gibbs free energy following mutagenesis were predicted, suggesting promising variants with higher stability under extremely acidic conditions. The rationally designed variant, A55R/N156E-H239E, demonstrated a 12.5-fold increased half-life under extremely acidic conditions, 9.9-fold increased catalytic efficiency toward veratryl alcohol, and a 7.8-fold enhanced lignin model dimer conversion efficiency compared to those of native LiPH8. Furthermore, the two constructed salt bridges in the variant A55R/N156E-H239E were experimentally confirmed to be identical to the intentionally designed LiPH8 variant using X-ray crystallography (PDB ID: 6A6Q). Conclusion: Introduction of strong ionic salt bridges based on computational design resulted in a LiPH8 variant with markedly improved stability, as well as higher activity under acidic pH conditions. Thus, LiPH8, showing high acid stability, will be a crucial player in biomass valorization using selective depolymerization of lignin

RNA interference-mediated knockdown of p21WAF1 enhances anti-tumor cell activity of oncolytic adenoviruses

The ability of oncolytic adenoviruses to replicate in and lyse cancer cells offers a potential therapeutic approach. However, selectivity and efficacy of adenovirus replication need to be improved. In this study, we present that loss of p21WAF1 promotes adenovirus replication and more effective cell killing. To test our hypothesis, we took HCT116 colon cancer cell lines carrying deletions of either p21WAF1 or p53, and infected these cell lines with wild-type adenovirus (WtD) or the oncolytic adenoviruses, ONYX-015 and Delta-24. We found that WtD, ONYX-015 and Delta-24 induced stronger cytopathic effects in HCT116 p21−/− cells compared with HCT116-WT cells. This was accompanied by increased virus production. siRNA-mediated knockdown of p21WAF1, and similarly of p27KIP1, in HCT116-WT cells also enhanced replication of and cell killing by these viruses. Furthermore, we found that TE7, an esophageal carcinoma cell line, also showed a strong cell-killing effect and virus production when p21WAF1 expression was suppressed by RNA interference before adenoviruses infection. Also, H1299 and DU-145 cells transfected with p21WAF1 siRNA showed higher virus production after ONYX-015 and Delta-24 infections. These observations suggest that p21WAF1 plays a role in mediating replication of oncolytic viruses with potential implications for adenoviral therapy of cancer

Anticancer Gene Transfer for Cancer Gene Therapy

Gene therapy vectors are among the treatments currently used to treat malignant tumors. Gene therapy vectors use a specific therapeutic transgene that causes death in cancer cells. In early attempts at gene therapy, therapeutic transgenes were driven by non-specific vectors which induced toxicity to normal cells in addition to the cancer cells. Recently, novel cancer specific viral vectors have been developed that target cancer cells leaving normal cells unharmed. Here we review such cancer specific gene therapy systems currently used in the treatment of cancer and discuss the major challenges and future directions in this field

Delta-24-RGD combined with radiotherapy exerts a potent antitumor effect in diffuse intrinsic pontine glioma and pediatric high grade glioma models

Pediatric high grade gliomas (pHGG), including diffuse intrinsic pontine gliomas (DIPGs), are aggressive tumors with a dismal outcome. Radiotherapy (RT) is part of the standard of care of these tumors; however, radiotherapy only leads to a transient clinical improvement. Delta-24-RGD is a genetically engineered tumor-selective adenovirus that has shown safety and clinical efficacy in adults with recurrent gliomas. In this work, we evaluated the feasibility, safety and therapeutic efficacy of Delta-24-RGD in combination with radiotherapy in pHGGs and DIPGs models. Our results showed that the combination of Delta-24-RGD with radiotherapy was feasible and resulted in a synergistic anti-glioma effect in vitro and in vivo in pHGG and DIPG models. Interestingly, Delta-24-RGD treatment led to the downregulation of relevant DNA damage repair proteins, further sensitizing tumors cells to the effect of radiotherapy. Additionally, Delta-24-RGD/radiotherapy treatment significantly increased the trafficking of immune cells (CD3, CD4+ and CD8+) to the tumor niche compared with single treatments. In summary, administration of the Delta-24-RGD/radiotherapy combination to pHGG and DIPG models is safe and significantly increases the overall survival of mice bearing these tumors. Our data offer a rationale for the combination Delta-24-RGD/radiotherapy as a therapeutic option for children with these tumors. SIGNIFICANCE: Delta-24-RGD/radiotherapy administration is safe and significantly increases the survival of treated mice. These positive data underscore the urge to translate this approach to the clinical treatment of children with pHGG and DIPGs

First evidence for Wollemi Pine-type pollen (Dilwynites: Araucariaceae) in South America

We report the first fossil pollen from South America of the lineage that includes the recently discovered, extremely rare Australian Wollemi Pine, Wollemia nobilis (Araucariaceae). The grains are from the late Paleocene to early middle Eocene Ligorio Márquez Formation of Santa Cruz, Patagonia, Argentina, and are assigned to Dilwynites, the fossil pollen type that closely resembles the pollen of modern Wollemia and some species of its Australasian sister genus, Agathis. Dilwynites was formerly known only from Australia, New Zealand, and East Antarctica. The Patagonian Dilwynites occurs with several taxa of Podocarpaceae and a diverse range of cryptogams and angiosperms, but not Nothofagus. The fossils greatly extend the known geographic range of Dilwynites and provide important new evidence for the Antarctic region as an early Paleogene portal for biotic interchange between Australasia and South America.Mike Macphail, Raymond J. Carpenter, Ari Iglesias, Peter Wil